TCBS agar was prepared for about 100ml and poured into petri plates, after solidifying, pure colonies of bioluminescent bacteria was streaked. The petri plates were observed after 24 hours. If there is presence of yellow color colonies, it is concluded as Vibrio spp. 3.12.4 Species Verification by Sub-culturing at 4°C using SWC agar media The pure culture of Bioluminescent bacteria was streaked on the agar media and refrigerate at 4°C. The Photobacterium phosphoreum will exhibit slow growth at
It is important that your swimming pool pump is checked from time to time. Your pump should be checked by a professional. This company can carry out an inspection on the pump. They can also clean the filters and other equipment used in water filtration. This can help in getting rid of contaminated water that can cause skin diseases.
After they were cut into 2.00 cm each we found the mass. We zeroed out the scale and weighed all four potato cores at once and recorded the mass. We then put those potato cores into the beaker of 75 mL of solution. With the potato cores in the beaker we then put a watch glass over the top of the beaker to minimize the amount of solution that evaporates. We let the potato cores sit in the solution overnight.
To clean the syringe, flush it by drawing 6 mL of distilled water. Step 2: Mix both test tubes , shake gently and time the reaction. Step 3: The same step as procedure 1, and step 3 which is to record the observed color step 4: use the palette/color chart to help you identify the observations you make. Safety precautions: Pull your hair back Safety eye goggles Closed toe
Using the micropipette, we transferred the appropriate volume of algae to the jars. We collected a sample of brine shrimp and added them to petri dishes in order to view them underneath a dissection microscope. Because we were testing the top-down effects, we decided on having
Carefully remove the comb and tape surrounding the plate and transfer the gel plate to the electrophoretic tank such that wells are towards the cathode 4. Pour 1X TAE buffer into the tank until the gel is completely submerged. Connect the electrode to the power supply 5. Load the plasmid DNA preparation and standard DNA marker into separate well with help of a micropipette or a syringe 6. Turn ‘ON’ the power supply and run at 100 V (10-15 mA).
Extra care was taken to not touch the plate with bare skin. Five spots were labeled on the line and each amino acid standard was spotted on the plate using a capillary tube. The standards included leucine, alanine, phenylalanine, and lysine. The final spot was an unknown mixture of three amino acids. After allowing the spots to dry, the plate was placed in the developing jar and allowed to develop.
Put the caps on the bottles and shake them until all the sugar is dissolved. Testing the Sugar Solutions All of the solutions should be the same temperature before testing Place the hydrometer in the 0% sugar bottle, record your reading in your notebook Repeat this step for the 5%, 10%, 15%, 20% and 25% sugar solutions. Rinse and dry the hydrometer between readings. Testing the Soda and Iced Tea Place the hydrometer in soda 1(coke). Record the value on the hydrometer in your notebook Remove the hydrometer and rinse and dry
The only way to know if a water supply contains bacteria is to have it tested. The Environmental Protection Agency (EPA) requires that all public water suppliers regularly test for coliform bacteria and deliver water that meets the EPA standards. There is no requirement to have private water wells, springs or other sources tested, it is up to the individual homeowner. For public water supplies, frequency of testing depends on the size of the population served. Bacteria test results are available from the supplier and there must be a public notification if the water supply does not meet the standard.
The students used test kits and other tools to test pH, temperature, phosphate, nitrate, turbidity, and dissolved oxygen. The results of the tests were that the Susquehanna River is indeed, very good and healthy. It was discovered that temperature directly corresponds with dissolved oxygen. It was also discovered that there are different types of fish that live in different temperatures. Introduction The problem the students need to solve is whether or not the river is healthy.
The same process was repeated but the net was at the 10-meter mark and the students started shuffling at the five-meter mark. In the capture-mark-recapture method, the crayfish were marked using blue and pink nail polish allowing for the crayfish to be dumped back into the sampling area. The removal method group kept all of the crayfish collected aside to ensure only the new crayfish were collected. The 10-meter portion of the stream was then collected four more times, giving a total of five separate collections for each
After cutting a block of the fungus, we placed it on the petri dished in the section that was appropriately marked for that specific strain. After the four sections were placed, the lid was placed on the petri dish, and then the dish was with tape to keep close, and wrapped in aluminum foil and placed into the correct bin to sit. After two weeks the petri dishes were ready to be observed. We used a toothpick to scrap lightly the top of the agar in the petri dishes, ensuring we were collecting from one of the dividing lines. With the spores on our toothpicks, we then smeared them onto a slide that was provided to us.