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Oil Seed Crops: Brassica

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Brassica is important oil seed crop commonly known as rapeseed or mustard whose productivity is limited by several biotic stresses and abiotic (Grover and Pental,2003; Dutta et al.,2005). In agriculture, losses to crop yield due to variety of potential phytophagus insect pests are one of the major causes of lowering in crop productivity. The yield losses in oilseed crop are mainly caused by mustard aphids, Lypaphis erysimi, which is one of the major sap sucking insect in India (Kumar, 1999; Patel et al., 2004; Singh and Sachan, 1994). The damage is mostly due to nymph and adult stages which suck away the plant sap. The insect suck the plant sap during flowering and seed formation which prevents the nutrient flow and fail to produce healthy …show more content…

These pests breed predominantly through parthenogenesis and as a consequence population of mustard pests increases in very short duration. Magnitude of yield deduction is influenced by the growth stages of crop during aphid infestation, duration and intensity of infestation on crops. The intensive use of insecticides and pesticides against these insect pests leads to devastating effects on environment and generating harmful effects on producers as well as on consumers (Jouanin et al., 1998). Therefore, efforts were made through conventional breeding programme to introduce the aphid resistance but success has been poor due to non -availability of inherent aphid resistance in germplasm collection of Brassica (Rogers, 1980, Yadav and Singh et al., …show more content…

Therefore the present study has been envisaged to prepare two gene constructs utilizing Lectin gene isolated from lentil (Lens culinalis cv.4076) and protease inhibitor gene isolated from Chickpea (Cicer arientinum ) and established an efficient Agrobacterium mediated genetic transformation protocol to develop transgenic Brassica juncea cv. Varuna against aphid resistance.. Present study has been effectively utilized for expression of lectin and Protease inhibitor genes using phloem specific promoter rolC to protect plants against aphid attack. Both the genes are isolated from edible crops and hence biopesticidal property of both the genes are expected to be safe for

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