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Bengal Plate Test Essay

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DEVELOPMENT OF AN IN-HOUSE ROSE BENGAL PLATE TEST FOR DIAGNOSIS OF BRUCELLOSIS IN GOAT Sani Mohammed Yahaya, Siti Khairani-Bejo, Zunita Zakaria, Ariff Mohamed Omar, Asinamai Athliamai Bitrus Department of Pathology and Microbiology Faculty of Veterinary Medicine, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia ABSTRACT Brucellosis, caused by Brucella melitensis, is a significant problem for both public and animal health worldwide. The Rose Bengal Plate Test (RBPT) antigen from Brucella melitensis local isolates were developed in this study. The performance of the assay was investigated using serum samples collected from goats. A total of 1063 serum samples obtained from goats were examined for the presence of antibodies against Brucella by in-house RBPT (LRBPT), commercial RBPT (Veterinary Laboratory Agency –VLA, UK) and Compliment Fixation test (CFT).The sensitivity and specificity was calculated using CFT as the gold standard. Out of 1063 goats sera analyzed 364 (34.24%), 335 (31.51%), and 373 (35.08%) were found to be positive by LRBPT, commercial RBPT, and CFT, respectively . The sensitivity calculated for the LRBPT, was 90.1% compared to commercial RBPT 85.0%. However, the specificity of the LRBPT was lower (95.9%), than the commercial RBPT (97.4%). …show more content…

While the specificity (97.4%) of the commercial rose Bengal plate test is higher than that of the in-house rose Bengal plate test (95.9%). However, the PPV of the in-house rose Bengal plate test (92.3%) is lower than the PPV of the commercial rose Bengal plate test (94.6%). In addition, the NPV of the in- house RPBT (94.7%) is higher than the NPV of the cRPBT

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