Cobalt Food Experiment

Role of Cobalt nutrition on Bush bean (Phaseolus vulgaris) under salinity stress condition during vegetative growth

Abstract

Bush bean (Phaseolus vulgaris cv. Sunray) is salt sensitive species and the purpose of the present experiment was study role of different concentration of cobalt (0, 4, 8 and 12 mg/L) in mitigation of salt stress (0, 25 and 50 mM NaCl) in bush bean. The experiment was design as factorial base on CRD (completed randomized design) with 3 replications. The results showed that both Cb applications enhanced chlorophyll a at serve salinity stress relative to non-Cb treatment. The highest chlorophyll b was at non-stress conditions in combination with 8 and 12 mg/L Cb. Carotenoids significantly increased by 4 mg/L Cb application

0.1 g of sample were soaked in dimethylsulfoxide (DMSO) about 24h in dark place. The solution absorption was recorded at 663, 645 and 470 nm using a spectrophotometer (V-530, JASCO, Japan).

Leaf electrolyte leakage was determined according to the method of Sairam et al. (1997). A 0.1 g leaf sample was placed into a vial with 10 mL of double distilled water in 2 series. The first and the second series were placed in an autoclave at 40°C for 10 min, then at 100°C for 30 min. Electrolyte leakage (EC1, EC2) was recorded with a conductivity meter (Jenwayv, England), separately. Then, electrolyte leakage was calculated using Sairam et al. (1997) formula.

Leaves were homogenized in 3% aqueous Sulphosalicylic acid, then centrifuged 6,000 rpm for 15 min at 4°C. 1 mL of this homogeny solution react acid-ninhdrin and 1 mL of glacial acetic acid in a tube for 1 hour at 100°C and the reaction is torn up in an ice bath and then extracted with 2 mL of toluene. It was kept at room temperature to stabilize. Proline (Pro) content was measured by spectrophotometer (V-530, JASCO, Japan) at 520 nm (Bates et al.,

Scientia Horticulturae 103, 93–99.

Bayuelo-Jiménes, J.S., Craig, R., Lynch, J.P., 2002. Salinity tolerance of Phaseolus species during germination and early seedling growth. Crop Sci. 42, 1584–1594.

Läuchli, A., 1984. Salt exclusion: an adaptation of legumes for crops and pastures under saline conditions. In: Staples, R.C., Toenniessen, G.H. (Eds.), Salinity Tolerance in Plants: Strategies for Crop Improvement. Wiley, New York, pp. 155–159.

Effect of salt stress on growth, chlorophyll content, lipid peroxidation and antioxidant defence systems in Phaseolus vulgaris L.

Khaled Taïbi a,c,d,⁎, Fadhila Taïbi a, Leila Ait Abderrahim a, Amel Ennajah b,

Moulay Belkhodja c, José Miguel Mulet. South African Journal of Botany 105 (2016) 306–312

Fig 1. Interaction effect of different Co concentration and NaCl on chlorophyll a

Fig 2. Interaction effect of different Co concentration and NaCl on chlorophyll b

Fig 3. Interaction effect of different Co concentration and NaCl on carotenoids

Fig 4. Interaction effect of different Co concentration and NaCl on MSI

Fig 5. Interaction effect of different Co concentration and NaCl on RWC

Fig 6. Interaction effect of different Co concentration and NaCl on