We use Mendelian genetics to study the genetics of C. elegans. C. elegans have very similar genetics structure to humans. C elegans belongs to Phylum Nematode species which is very different from the earthworm. C. elegans is the first eukaryotic organism to have an entire genome sequence. It is very easy and simple to conduct an experiment on C. elegans that’s why the majority of laboratories use this organism. The reason for using the C. elegans in an experiment and in research’s is because they are harmless it humans they are non-parasitic. C. elegans grows on. E. coli which can be easily found in rotten fruit or vegetables. This organism is very small and can be handled easily the adult C. elegans is about 1mm in length, C. elegans have …show more content…
elegans with two petri dishes. One Petri dish contains homozygous hermaphrodites and homozygous dpy-3 -/-, his-8 -/- C. elegans. The second dish contains dpy-3 +/, his-8 -/- male. We had another dish with only E. coli which we used to transfer C. elegans from one Petri dish to another. The materials that we used when conducting this experiment are: dissecting microscope, 3 petri dishes, C. elegans (male, female, and hermaphrodites), worm pick tool, agar, Bunsen burner, Parafilm, incubator, and flint spark lighter. The methods of this experiment are really simple. When we started the experiment we all washed our hands and wore gloves. Each group member did their part to conduct and successful experiment, one group member plugs the Bunsen burner to the gas pipe and turned on the gas, then used flint spark lighter to set the flame on the Bunsen burner. While the second group member is setting the dissecting microscope and making sure the lenses are clean. This member is getting all three Petri dishes ready to examine (first Petri dish contains E. coli, the second dish have the mixture of C. elegans, the third dish is where we are transferring female C. elegans to mate up). Then we took turns to examine the C. elegans under the dissecting microscope. When looking C. elegans under the microscope w first need to identify which one is male, female, dumpy and hermaphrodites Male has zig-zag shaped tail and males tend to be smaller than females. Females have sharp, and point tails they tend to be mostly long. The dumpy C. elegans is round and short oval shape tail dumpy are tend to little fat than others. The last type of C. elegans are hermaphrodites are big and fat with point tails they are very similar to male C. elegans. Due to hermaphrodites, transparent bodies we can identify with the difference between them and male C. elegans hermaphrodites have a uterus, and gonad both male and female sexes are visible. As in the article, it says “male C. elegans
This experiment was conducted to determine whether or not Callosobruchus maculatus, or bean beetles, had a bean color preference for oviposition choice. Oviposition is the process of a female insect laying her eyes on plant parts and other materials, which can be influenced by many factors. The bean beetle eggs are opaque and clear, which allowed us to test the hypothesis that C. maculatus prefer the darker red Adzuki beans over the white Black-eyed peas for oviposition choice. Two different colored bean types were used, the red Adzuki beans and the white Black-eyed peas. We placed three female and two male bean beetles in each petri dish, with 55 of each bean type randomly placed in the dish, for a week.
Introduction C. elegans are 1mm transparent worms used in many experiments as a model organism, since they are creatures that are easy to use and take care of in a laboratory setting. These organisms are small in size, have a short three-day life cycle, and reproduce efficiently in the lab. The C. elegans only have two sexes, male and hermaphrodite and these sexes make the organism convenient for breeding and reproduction in the laboratory environment (Hope IA, 2005). One reason the C. elegans is so highly used in experiments is that it has the ability to detect some volatile chemicals with a process called chemotaxis; this organism is shown to chemotax towards certain compounds. Chemotaxis is the gradient formed by the movement of organisms towards an odorant, also known as a substance with a certain fragrance.
Transformation in bacteria usually takes place when a bacterial cell accepts strange DNA and integrates to its own DNA. The transformation normally takes place within plasmids, which are tiny circular DNA molecules that have been separate from its own chromosome. The copies of the same plasmid range from 10 to 200 copies within a cell. These copies of plasmids may multiply when the chromosome replicate or multiply independently. One plasmid has a range of 1,000 to 200,000 base pairs.
The lab starts with me being able to choose from four different environments that I want to put my organisms in. I also get to choose one of the four different allele frequencies that are allowed. I can mix and match the environment and frequencies however I want to. After picking an environment and a frequency, I then am able to move one generation forward. When I move a generation forward, the allele frequency will change.
At each station, there are four graduated cylinders, two of which acted as control groups, and two for the two spritesand muscle milk. You would put drops of the solution, that identifies if a certain macromolecules are present varying on each station, into each of the control groups. The control groups were a substance that contains the macromolecule which is being tested for and a substance that does not. You would then compare the sprite and muscle milk that you added the solution to, after stirring each graduated cylinder, and see which control group it represents most
For this experiment, we studied how planarian worms would grow after being segmented. To begin this experiment, we filled up petri dishes half-way with spring water, this is so the planarian worms would not dehydrate and die during the two weeks we were observing them. Next, the planarian worm was picked up from its container with a pipette and then placed in a separate petri dish with some water. This took some time and I ended up having to use a pick to get the planarian worm off the inside walls of the pipette. Once the worm was in the petri dish, a razor was used to cut the worm in half, creating a “head” and “tail” segment of the worm.
The purpose of this experiment was to determine if there is natural selection against blind, white-eyed male Drosophila melanogaster, as well as if there is any interplay between selection and drift occurring in populations of different size. This was done by creating and monitoring both small and large populations and placing them in an environment with regular light or complete darkness. It was predicted that natural selection would occur against white-eyed males in the light trials, but would not occur in the dark trials. This selection would occur on the level of male mating success and be seen in a decline in the frequency of Q in the light trials. It was also predicted that drift would be larger in small populations, which would be seen
CO2 tanks, as well as CO2 pads, dissecting microscope, pencil, vials/test tubes, fly food, paint brushes or a spatula, a dawn filled cup of death (morgue), and live Drosophila melanogaster will all be necessary. Starting with two vials full of food to sustain the flies/pupa, we began with the parent generation already in the vials in the pupa phase. After all the flies had enclosed, having emerged from the pupa stage into adulthood, we were ready to examine them. Each group turned on their CO2 pads, and prepared to open the test tubes. Placing the test tubes upside down, and tapping the glass to make the remaining flies fall to the CO2 pad.
So as they were developing this experiment they needed to gather
The student had to make a trap door so they could check the temperature and the pH. Then added holes so the worms could breathe. There were 3 sections, the aquatic, decomposition, and terrestrial. The decomposition people had to check
5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).
2 of the petri dishes were soaked in water, and the third was soaked in liquid Asian ginseng, for 5 minutes. The seeds of each individual petri dish were placed, wrapped and rolled in 3 different paper towels. The wrapped paper towels were set into 3 different red solo cups with the seeds at the top of the cup and 20ml of water on the bottom of the cup.
Mycelia are a network of long hyphae filaments which the fungus uses to form sex organs. The newly formed, diploid nucleus must go through mitosis to become haploid again. This will produce eight haploid ascospores held in the ascus.
Conclusion In conclusion my hypothesis was right and the wet bread grew the most (and only) mould. I can logically assume that the more moisture the bread has, the more mould it will grow. This was a fair test because each of the bread pieces had equal time to grow. The End In this experiment the conductor finds out that mould needs moisture thus needs to be included to conduct the experiment.