CHAPTER 3 METHODS AND PROCEDURES This chapter deals with the methodology used to identify the anticoagulant property of the sulfated glycosaminoglycan extract from the White Leg Shrimp (Penaeus vannamei, family Penaeidae). Research Methodology The research method used in this study is experimental. The sulfated glycosaminoglycan extract was obtained through extraction and isolation. Physical and chemical tests were conducted to identify the sulfated glycosaminoglycan. Instrumental tests were performed to identify and quantify the sulfated glycosaminoglycan.
The hydroxyl group (-OH) of NaOH attacks an electrophilic carbon of >N-C=O which as rearrangement gives carbonial . This carbonial abstract proton from water to give NAG. The established over degradation of NAG to 4-MBA was also obseved in alkali condition. Degradation pathway of AN is shown in Fig.3. The isolated degradation products are subjected to Mass studies to obtain their accurate mass fragment patterns.
,  The uses of BS agar are for the inhibition of Gram-positive bacteria and coliform by the presence of BS indicator and brilliant green. BS agar allows growth of Salmonella spp. Enzymatic digest of Casein, Enzymatic of Animal tissue and beef extract in the agar supply sources of nitrogen, carbon, and vitamins essential for the organism growth. Dextrose acts as the carbohydrate source and Disodium Phosphate as the buffering agent. The production of Hydrogen Sulfide is from the Ferrous Sulfate where the iron is precipitated and results in brown to black colour with metallic sheen colony.
Journal of Zoology, London, 252, 473-480.s Somers, M. J., and Nel, A. J. J., 2004, Movement Patterns and Home Range of Cape Clawless Otter (Aonyx capensis), Journal of Zoology, London, 262, 91-98. Tomlinson, D. N. S., 1974, Studies of the Purple Heron, Part 1, Heronry Structure, Nesting Habitat and Reproductive Success, Ostrich, 45, 175-181. Toumi, P., 2001, Sea Otters, in Dierauf, A. L., and Francis, D. M., ed., Marine Mammal Medicine Health, Disease and Rehabilitation, 2nd edition, Gulland, CRC Press, pg.,
Biosorption of Chromium Using A. towneri and R. eutropha J. Aravind Asst. Prof. (SrG), Department of Biotechnology, Kumaraguru College of Technology, Coimbatore-641049 G. Ligi Elezabeth, T. Mariya ABSTRACT Chromium is one of the toxic heavy metals which are discharged into the environment from various industries which causes serious threat to human health as well as environment. Biosorption is one of the effective methods for removal of chromium which uses dead or inactive biological matter. In the present study, batch biosorption experiments were carried out using Acinetobacter towneri and Ralstonia eutropha. Various parameters affecting biosorption such as temperature, pH, adsorbent dosage, contact time and initial metal ion concentration
Like OPC-67683, Nitroimidazo-oxazine is a nitroimidazole that has demonstrated bactericidal and sterilizing activity against drug-resistant and non drug-resistant TB. Nitroimidazo-oxazine has also shown activity against both active and latent TB. In a 2002 agreement with the former biotechnology company Chiron, The TB Alliance is carrying out phase II clinical testing on Nitroimidazo-oxazine (Diacon et al. ). Nitroimidazo oxaine is also active against latent TB bacteria .In a latent state, bacteria are anaerobic and else replicating very slowly and non replicating.
VARIATION OF FLOW RATE IN CASE OF MHD FLOW OVER A MOVING INFINITE VERTICAL POROUS PLATE IN THE PRESENCE OF THERMAL RADIATION AND UNIFORM HEAT FLUX 1J.V.Ramana Murty 2K. Jhansi Rani and 3Ch. V. Ramana Murthy¬, 1National Institute of Technology Warangal-506004 2Lakireddy Balireddy College of Engineering, Mylavaram – 521230 (A.P. ), India email@example.com 3Vasavi Institute of Engineering and Technology, Nandamuru – 521369 (A.P) India firstname.lastname@example.org Abstract: The case of variation of flow rate with respect to various flow entities in situation of MHD flow over a moving infinite vertical porous plate in the presence of thermal radiation has been examined in detail in this paper. The governing equations of motion have been solved to the
EFFECT OF MERCURIC CHLORIDE TOXICITY IN ACETYLCHOLINE ESTERASE LEVEL IN Danio rerio Jyotsna, Alok Kumar Madeshia, B.Praba Sheela Department of Biotechnology, Vinayaka Missions University Paiyanoor ,Chennai,Tamil Nadu,INDIA. Corresponding Author’s email:email@example.com ABSTRACT: Fishes are used as bio indicators to assess the quality of water pollution. In the present study we aimed to check the influence of mercuric chloride in acetyl cholinesterase level in brain of zebra fish. Various sub lethal dosage of mercuric chloride altered the enzymatic level in brain. The Level of Proteins in brain was also reduced due to toxicity.
MOLECULAR CHARACTERIZATION OF SALMONELLA ENTERICA FROM TONSIL AND JEJUNUM OF SLAUGHTERED SWINE Corinne C. Bautista, 1 and Windell L. Rivera, Ph. D.1, 2* 1Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City 1101, Philippines 2Molecular Protozoology Laboratory, Natural Sciences Research Institute, University of the Philippines, Diliman, Quezon City 1101, Philippines *Corresponding author: firstname.lastname@example.org This study is part of “Diagnosis and Molecular Characterization of Salmonella Species from Slaughtered Swine and Other Raw Meat and Meat Products” project funded by Department of Agriculture-Biotechnology Program Implementation Unit (DA-BIOTECH) and undergraduate thesis of Ms. Corrine C. Bautista in Institute of Biology, University of the Philippines Diliman. MOLECULAR CHARACTERIZATION OF SALMONELLA ENTERICA FROM TONSIL AND JEJUNUM OF SLAUGHTERED SWINE ABSTRACT Foodborne illnesses like gastroenteritis caused by Salmonella are a major health concern in the Philippines. Animal processing and butchering in slaughterhouses
BMC Complementary and Alternative Medicine. 2008; (8): 27. Ferrali M, Signorni C, Ciccoli L, Comporti M. (1992).Iron release and membrane damage in erythrocytes exposed to oxidizing agents phenylhydrazine, divicine and isouramil. Biochemical Journal. 1992; 285 (1) 295-301.