Cacacia Catechu Case Study

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6 Acute toxicity study ofAcacia catechu seeds 6.1 Introduction Acacia catechu Willd (Mimosaceae) is commonly called as khair or black cutch. It is known as khadira in Sanskrit and Karungali in Tamil. People in Kerala drink the boiled juice for digestive disorders (Singh, 2006) . In India, there are the three varieties of A. catechu, namely catechu, catechuoides, and sundra. Catechu (heartwood) is commercially used to obtain katha a concentrated filtered extract in North India (Quadry, 2009). The extracts of various parts of A. catechu exhibit variety of pharmacological effects such as analgesic, antipyretic, anti-inflammatory, antidiarrheal, hypoglycemic, hepatoprotective, gastroprotective, hypotensive, anticancer, antioxidant, and…show more content…
1% hydroxypropyl cellulose solution was prepared in distilled water. Various concentrations of the seed powder were suspended in the solution and warmed to about 40°C and vortexed. The suspension was administered to the rats using a 16 gauge oral feeding cannula. Initially, a dose of 800 mg/kg was given to four rats. Once the dose was tolerated further doses of 1600 and 3200 mg/kg were given (logarithmic dose). All doses were given as an single dose. The control group received hydroxylpropyl cellulose. The animals were weighed daily. General behavior,food and water consumption were monitored for a period of 14 days as per OECD guidelines. At the end of the experiment, the animals were anesthetised with isoflurane and blood was withdrawn from the orbital plexus for various haematological and biochemical analysis with and without anticoagulant (K2 ethylenediamine tetraacetic acid). They were sacrificed by cervical dislocation and vital organs were removed, freed from extraneous tissues, observed for any gross pathology, weighed, and preserved in 10% formalin. 6.2.6 Hematological analysis : Whole blood was subjected to various hematological analysis using Beckman Coulter Cell Counter (UK). The following parameters were determined: red blood cell count, white blood cell count, hemoglobin (Hb) concentration, packed cell volume (PCV), and platelet count (PLT). The procedure was followed as per…show more content…
All the animals appeared healthy without any apparent symptoms suggesting that the compounds are well absorbed in the digestive system. Evaluation of various hematological parameters did not reveal any toxicity of the seed extract except a decrease in platelet count at 3200-mg/kg dose. Interestingly, in the present study, an increase in hemoglobin and PCV was observed in all the doses showing that the ethanolic extract of the seed may have some beneficial effect. Hepatotoxicity, cardiotoxicity, and cell damage are generally assessed by the estimation of enzyme levels, namely aspartate aminotransferase, alanine aminotransferase, ALP, gamma glutamyl transaminase, and lactic dehydrogenase. The above marker enzymes were evaluated in serum at the end of 14 days for a high dose of A. catechu seed extract, and no change was observed showing that the seed extract has no delayed toxicity. Natural products are known to cause cell damaging effect for example Fagonia cretica Induces DNA Damage, Cell Cycle Arrest and Apoptosis in Breast Cancer Cells via FOXO3a and p53 Expression and hot water extracts of Chlorella vulgaris showed anti- proliferative and apoptotic effects on hepatoma cell line HepG2 (Dickers et al,

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