Pepsin (1 wt. % of the estimated collagen) was added to the supernatant, stirred for another 2 days. Soluble collagen was purified by a repeated process of salting-out with 1 M NaCl, condensation by centrifugation at 14000 ×g for 20 minutes and subsequent solubilization in 0.5 M acetic acid. The collagen solution thus obtained was dialyzed in dialysis tubes (VISKING dialysis tubing, MWCO 12 000 - 14 000 RC, diameter 21 mm) against sterile 0.1 M acetic acid and sterile distilled water, respectively and
Dialysis membrane having a pore size 2.4 nm and molecular weight cut off between 12,000 and 14,000 was used. The membrane was soaked in double distilled water for 12 hr. before mounting in a Franz diffusion cell. About 1ml of semisolid preparation of NLC was applied to the donor compartment, and the receptor compartment was filled with phosphate buffer, pH 7.4 (14 ml). During the experiment, the solution in the receptor side was maintained at 370C and stirred at 800 rpm with Teflon coated magnetic stirrer bar.
Incubated at 25℃ for 7 days under the shaking condition. After 7 days, centrifuge it at 5000rpm and at temperature of 4℃ for 20 minutes. Discard the pellet that contains bacterial cells, take supernatant. Make the pH of supernatant of 2 by using "M" "H" _2 "S" "O" _4. Add choloroform and methanol of ratio 2:1 and of equal volume.
Animals were also weight immediately prior to sacrifice (fasted body weight). Animals were sacrificed under anesthesia with diethyl ether, and then blood samples were immediately collected in clean and dried Wiesserman tubes from the portal vein. First part of blood was collected in tubes containing potassium oxalate and sodium fluoride for the estimation of plasma glucose by O-toluidine method of Sasaki et al. (1972). Second part of blood was left to coagulate then centrifuged at 3000 rpm for 15 minutes to obtain serum to estimate some biochemical parameters.
Then seedlings were placed into petri plates on filter paper with different melatonin concentrations of 100, 10, 1 and 0.1 mM in 0.02% dimethylsulfoxide. Control plants contained only distilled water. The results indicated that 0.1 mM melatonin treatment had impact on the growth of root whereas 100 mM had no effect on root growth.
Materials and Methods Preparation of test extracts Fresh plants of the mistletoe growing on Mangifera indica were collected during flowering in the month of August, 2009 from Kasaragod District of Kerala. Voucher specimen of the plant (no. 00637) was deposited at the Pharmacognosy department of Captain Srinivasa Murti Drug Research Institute for Ayurveda, Chennai. Shade dried whole plant material was extracted with ethanol and double distilled water by cold percolation. The aqueous extract (HEAq) was concentrated and dried in freeze drier to obtain dark brown gummy material and the alcoholic extract (HEAl) was concentrated over a water bath to obtain dark brown gummy material.
Known for its fragrant aroma and slightly bitter taste, turmeric is a common culinary spice in Indian cuisine. Turmeric also comes in liquid extract, capsule and powder forms. The traditional Oriental medicine systems have long used turmeric powder to treat an assortment of therapeutic conditions. Turmeric 's underground stems (rhizomes) are dried and made into capsules, tablets, teas, or concentrates. Turmeric powder is likewise made into a glue for skin conditions.
The natural agar consist of Yeast Extract, Tryptone , Lactose , Manniiol, Sodium Chloride , Dip otassium Hydrogen Phosphate ,Gelatin and Agar .Then , in a conical flask 14g of nutrient agar was mixed into 500 ml of distilled water and , The mixture was stirred and dissolved until most of the agar dissolve. By non-absorbent cotton wool plug the mouth of the flask was closed. By using the autoclave the agar was sterilized for 15 minutes.The cotton wool was removed. By the flame the mouth of the flask was heated before and after pouring the agar into the Petri dishes. And, the left hand the lid of the Petri dish was lift, just enough to enter the mouth of the flask and quickly was poured in agar (about 15 cm3).
The leaves are used in kidney troubles and in muscular pain and are applied on boils and carbuncles. Infusion of plant is used against rheumatism, cold and bronchitis [Srinivasulu C and Indraneil Das. 2008]. In Unani medicine, a confection of tender leaves and purified sugar is prescribed in anuria, retention of urine and kidney troubles The aim of the present study is to detect the phytochemical and investigate the antifungal spectrum from natural resources and to support the traditional uses ofGardenia
The selected streptomyces culture was inoculated into the broth. The tubes were incubated 4°C, 10°C, 20°C, 28°C, 37°C, 45°C, and 60°C for 8-10 days. The growth of the inoculated cultures was recorded after incubation. Effect of NaCl (Tresner et al., 1968) Yeast extract-malt extract agar was supplemented with Nacl at different concentrations 4%,6%,8%,10%(W/V) and autoclaved.After autoclaving, the medium wsa poured into sterilized petriplates.A loop full of spore suspensipn of each selected streptomyces species was streaked on the solidified agar plates divided into five sectors and incubated for 8-10 days. After the incubation period, the sodium chloride tolerance of the selected streptomyces was determined.