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Ajinomoto Fast Food

1323 Words6 Pages

Title: Quantitative role of calcitriol in Ajinomoto induced obesity in rats
AUTHORS: Shamsa kanwal, Sabra Sohail, Hafiza Tooba hashim, Rouhma Aziz,Maha Shahid,Mahnoor motiwala,Shajia Shuja,Maham baqai
Abstract: In our population there is a vast consumption of fast food in every age group .The main component in fast food that causes obesity is Ajinomoto. The insufficiency of vitamin D is correlated with obesity and metabolic disorder. For this reason our aim is to study the quantitative role of calcitriol in Ajinomoto induced rats
Objective: to design fast food containing vitamin D for fast food junkie and also for vitamin D deficient population.
Introduction: obesity is unrestrained aggregation of fat that may undermine the physical fitness …show more content…

SELECTED ANIMALS FOR RESEARCH:
For this research Rats(120-200 g, body weight) are chosen, they are retained in zoological dwelling with 12:12 luminosity, aphotic period at 26+ 2 degree C .they will be held in isolated plastic enclosure consists of antiseptic paddy rind as sheet substance.

2.ESTIMATION OF AJINOMOTO DOSAGE:

Ajinomoto is attained as crystals; Dissolve 250g of Ajinomoto in 500 ml saline for making stock solution. Animal body weight is important factor for dosage estimation (5g/kg) the estimated dosage of Ajinomoto is induced by oral gavage.
3.Estimation of calcitriol dosage:
Calcitriol is a synthetic vitamin D, analog which is active in the regulation of absorption of calcium from the GIT and its utilization in the body. It is mostly available as capsule. 1 mg/mL of Calcitriol (1, 25-dihydroxycholecaliferol) ampoules manufactured by pharmaceuticals Ltd are mixed in normal saline to result in a concentration of 0.06 mg/mL. As per the body weight of rats, dosage will be calculated. Calcitriol will be administered through oral gavages (0.2 mg/kg bodyweight).
4. Animal grouping: In our study, the animals were divided into three groups:
Group I - the control group
Group ll -the group which receives only …show more content…

TIME TABLE FOR THE PROJECT:-
Research Activities Week 1 Week 2 Week 3 Week 4 Week 5
Animal selection and maintenance Preparation of physiological solutions for rats Insertion of saline,MSG and MSG with vitamin D to our chosen rats Observe the effects on Rats and data collection and data analysis Repeat the procedure for accurate

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