Introduction: Intra venous therapy for infusion of blood products, fluid and electrolytes, parenteral medications, hemodynamic monitoring, and for total parenteral nutrition (TPN) has become an essential feature of modern medical care. However, this is associated with the significant risk of infection of blood stream
Material and Methods: Intravenous cannulae with complaint of shooting pain and tenderness associated with cannulae are chosen as subjects of study. Tips of the cannulae were cultured using the semiquantitative method described by Maki.
Results: Among them, 32(32%) cannulae were infected, Among the blood samples of these patients cultured, 14(14%) were positive and 86(86% ) blood samples were sterile. Fourteen were Coagulase
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*P Value < 0.0001
A total of hundred patients who were admitted into various units in Fathima hospital, Kadapa are selected as subjects of the present study. All of them had indwelling intravenous cannulae, for diagnostic or therapeutic purpose. Cannulae were removed and cultured, from those who complained of pain and swelling at the site of cannulation. Among them, 32(32%) cannulae were infected, among the blood samples of these patients cultured, 14(14%) were positive and 86(86% ) blood samples were sterile.
Relation of culture positivity with pyrexia The relation of culture positivity with fever: Out of 32 patients with positive cannula cultures, 10 (31%) had pyrexia after cannulation , whereas out of 14 patients with positive blood cultures, 6(42.8%) had fever after cannulation .
Table No. 1 shows the list and number of isolates from cannula culture. Fourteen isolates were Coagulase negative Staphylococci, ten were Coagulase positive Staphylococci and five were Micrococci. The others were Corynebacterium jeikeium(4), Candida parapilosis(2), Candida glabrata(2), Klebsiella pneumoniae(2) and Pseudomonas aeruginosa
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24 samples were collected from patients suffering from central nervous system disorders among them, 6 (25%) each of cannulae and blood showed positive culture. 15 samples were collected from patients suffering from musculoskeletal disorders and among them, 7 (46%) cannulae were positive for culture and all samples of blood from the same patients were sterile. Among 14 samples processed from patients suffering from bronchopulmonary disorders, 3 (21%) cannulae and 2 (14.2%) samples of blood were
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
The purpose of this lab report is to employ a myriad of skills, tools and, methods learned throughout this semester to perform the appropriate tests for the identification of the assigned unknown bacteria. Add more background information here!!! The most important tools and techniques used during this identification include aseptic technique, microscopic examination and, the use of selective and differential media. Aseptic technique is an important tool for microbiologists. It is imperative that aseptic technique is maintained throughout the length of any test to avoid any cross-contamination that may lead to inaccurate results.
In this experiment, we cultivated an unknown specimen containing two microorganisms. The purpose of this experiment was to use a variety of biochemical test previously learned in the lab to identify the unknown bacteria. The identification of unknown bacteria is a major part of microbiology. Microbiologist observe samples such as blood and sputum in the laboratory for the presence of microorganisms. Identifying unknown bacteria is extremely important in clinical settings because it helps physicians find treatment for infections.
Unknown Lab Report Abiola Oyewumi March 16, 2015 Unknown #16 Abstract An experiment was conducted to determine which of the following unknown bacteria was in test tube number 16: Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Salmonella typhimurium. Biochemical tests were used to help identify the unknown bacteria. The Citrate test, Urease test, Triple Sugar Iron Agar test, Voges-Proskauer test, and Methyl Red test were the biochemical tests used in this experiment.
In the laboratory, identification of an unknown bacterium is often necessary. In the lab, a random sample consisting of three different bacteria was selected. The sample contained one gram-positive, one gram-negative paracolon, and one gram-negative coliform. The purpose of the experiment is to identify each of the three species that the mixture contained. After receiving an unknown mixture, the sample was streaked for isolation onto TSA, blood agar, and MacConkey plates.
Article Summary The impact of CAUTI remains a major threat to the safety of the patient and accounts for about 40% of hospital-acquired infection. The main culprit associated with the occurrence of CAUTI is having an indwelling catheter during a hospital stay. According to the Centers for Medicare and Medicaid Services, CAUTI’s are well thought-out to be an avoidable barrier of hospitalization, hence no extra payment is afforded for this treatment.
By Gram staining alone, it was safe to eliminate the three Gram positive bacteria that could have been assigned: S. epidermidis, M. luteus, and B. megaterium. The second step was to streak plate Unknown #10 to observe its macroscopic
The patient is a 52-year-old gentleman who is admitted inpatient because of redness of the left leg with pain and itching. The patient in fact was scheduled for an outpatient cardiac. He had a positive stress test. He was actually brought for catheterization and coronary angiogram the day of admission, however when seen in the cath lab the procedure had to be canceled because of a roaring cellulitis of his left leg. It started 1 week prior to admission.
However, 3 species out of 14 mentioned above are capable of causing pathogen infections. In fact, with few exceptions, staphylococcus belongs to the facultative anaerobes. More virulent staphylococci produce plasma coagulation. Staphylococcus Aureus plays the mot significant role in human pathology. Its colonies are larger than colonies of Staphylococcus Epidermidis.
Delayed and acute transfusion reactions were observed in most of the cases and some cases of death. Transfusion-related mortality identified transfusion-related acute lung injury (TRALI) is cited by Vamvakas and Blajchman (2009) as the leading cause of mortality among individuals who went through blood transfusion followed by non-ABO and ABO non hemolytic transfusion reactions and sepsis related to transfusion reaction. The establishment of factors that cause transfusion reactions have been studied. In the study of Honig and Bove (2003) on Transfusion-Associated Fatalities they found out that 70 cases of fatalities related to transfusion reaction were caused by incompatibility in ABO grouping and clerical confusion.
The second ½ of the organism was used for gram staining. The gram stain method was performed on the unknown organism per lab manual page 42 and two gram stain reactions were identified. Organism B was gram positive cocci in grape like clusters. Because organism B was positive I could eliminate Escherichia coli, Enterobacter aerogenes and Proteus vulgaris because these bacteria would be rod shapes. Organism A was gram negative pink rod shaped and because of gram positive morphology I could eliminate Staphylococcus aureus, Streptococcus lactis and Bacillus subtilis.
Treatment at a facility that is capable of treating an envenomation includes keeping the patient calm and trying to lower his heart rate while marking the swelling and recording the time on the patients arm to see how fast the swelling is occurring, an Iv should be established so that the medications can be administered. The administration of Crotalidae Polyvalent Immune Fab (Ovine) is the medication that should be administered, the medication has to be reconstituted, and the initial does is 4-6 vials for adult and pediatric patient’s with the start of the medication being infused over 60 minutes, proceeding slowly over the first 10 minutes at a 25 to 50ml/hr with careful observation for any allergic reaction. If the patient does not have
1.0 INTRODUCTION In the recent time increase in multiple drug resistance by microbial pathogens become a serious health problem to mankind worldwide. The increase has slowed down the development of new synthetic antimicrobials drugs and has necessitated the search for new antimicrobials from alternative source (Peng et al., 2006). It is aroused due to indiscriminate and repetitive use of antimicrobial drugs coupled with inadequate diseases treatment (Shariff, 2001). To acquire drug resistance microbes have developed series of mechanism to render drug useless for the treatment and control of infections (Ritch-Kro et al., 1996).One way to prevent antibiotic resistance is by using new compound which
Aseptic technique was initiated at the beginning of this experiment by cleaning the work surface with disinfected wipes. Personal protectives equipment was also worn. The material utilized in this experiment was: S. epidermidis culture broth, sterile cotton swab, streak plate, forceps in 70% alcohol, a lit tea light, and the three antibiotic disks (novobiocin, gentamicin, penicillin). The first step, I divided a plate into three quadrants and labelled them with the different antibiotic names. Using the lit tea light, like a bursen burner, I flamed the mouth of the S. epidermidis culture.
The risk of bloodstream infection in adults with different intravascular devices:a systematic review of 200 published prospective studies. Mayo Clin Proc.81:115971. PMID: 16970212. 7. Francischetto O, da Silva LAP, Senna KMS e, Vasques MR, Barbosa GF, Weksler C, et al (2014).