ipl-logo

Amide Bond In Capsaicin

1542 Words7 Pages

Acid Hydrolysis of Amide Bond in Capsaicin Abstract Introduction Capsaicin or 8-methyl-N-vanillyl-6-nonenamide is an active component of chilli peppers. Peppers are fruits from plants from genus Capsicum and belong to the family of Solanaceae.[1]Capsaicin occurs in plants found in the capsicum family, including bell peppers, jalapeno peppers, cayenne peppers and other chili peppers. The type of chilli used to extract capsaicin is red chilli padi or bird’s eye chilli and dried chilli, as it is commonly added to many foods as a spice. This type of chilli is from the capsicum annum family and it is mostly found in the regions of Ethiopia and Southeast Asia. Red chilli padi has a Scoville scale of 100,000 to 225,000 SHU and is highly pungent. Pure capsaicin is a non-volatile, hydrophobic, colorless, highly pungent, …show more content…

From the start of the experiment, we have restricted our aim by merely conducting acid hydrolysis of amide bond in capsaicin using citric acid to test for the decrease in the amount of capsaicin in chillies. By making the concentration of citric acid differ from each other, we would dilute them to different extents (such as 1.0 mol/dm^3, 1.5 mol/dm^3, 2.0 mol/dm^3) other than the usual concentration used in our experiment (0.5 mol/dm^3). Since the independent variable will become the concentration of citric acid, we would be able to further study the effect of the concentration of citric acid on the amount of capsaicin reduced in the chilli, which is obtained by comparing the capsaicin peaks on different concentrations of citric acid, and to observe trends of the capsaicin peaks i.e. As the concentration of citric acid added to capsaicin increases, the capsaicin peaks are lower, indicating reduced amounts of capsaicin in the chillies. From then, we are able to conclude on the overall effectiveness of citric acid on

Show More
Related

  • Peroxidase Chemical Reaction Lab

    740 Words  | 3 Pages

    While conducting this experiment readings were took every 15 seconds for 5 minutes which equals to a total of 20 readings. This experiment had four different trials, a base line trail, a pH 4 trail, a pH of 7 trail, and a pH of 8 trail. Then the results were recorded. The enzyme peroxidase was taken from the inside of a turnip (2g), then blended with 150ml of deionized water, and then poured through a coffee filter into a beaker for a smooth solution. Secondly the colorimeter had to be calibrated by placing a clean cuvette only containing the enzyme solution.

    Read More

  • Enzyme Lab

    901 Words  | 4 Pages

    After record your data and determine the absolute rate of the enzyme-catalyzed reaction. Based on the data and observations the hypothesis was accepted. It was accepted because when pH were changed to a variety of levels the transmittance began to get higher reaction rates. The increased absorbance means greater amount of product and a higher reaction rate will be produced.

    Read More

  • Chemical Reactions Lab Report

    454 Words  | 2 Pages

    Factors that affect chemical reactions Intro: this experiment is to show us what factors affect the rate of a chemical reaction. Supplies: The Works Liquid Toilet Bowl Cleaner, TUMS antiacid tablets, a non-serrated knife, a spoon, a stirring rod, four test tubes, flame heater, a small beaker for the boiled water, rubber gloves, and safety goggles. Procedure: we filled a beaker 3/4 of the way full with tap water and brought the water to a boil. We then labeled our test tubes 1,2,3,4.

    Read More

  • Turnip Peroxidase Lab Report

    2136 Words  | 9 Pages

    By completing this experiment, knowledge collected about optimal pH in enzymes will help

    Read More

  • Preparation Of L-Phenylalanine Lab Report

    802 Words  | 4 Pages

    “Diazotization of L-phenylalanine results in the unstable aliphatic diazonium salt 2, which is believed to undergo a rapid, intramolecular SN2 reaction to give the highly strained R-lactone (3) (3)”. “In a second, slower, intermolecular SN2 reaction, 3 reacts with the solvent (water) to open the lactone and yield the final product, (S)-2-hydroxy-3-phenylpropanoic acid (4)”. “Because this process occurs with two SN2 reactions, the final product has a net retention of configuration”. “This reaction has the added advantage of being environmentally friendly: the reaction is run in aqueous solution, using a safe amino acid and generates no hazardous waste requiring disposal”. “This experiment illustrates some important chemical concepts, including: Water solubility dependence on the state of ionization of a compound, Stereospecificity of the SN2 reaction, Measurement of optical activity, Effect of diastereotopic protons in the 1 H NMR spectrum”.

    Read More

  • Acetylsalicylic Acid Synthesis Lab Report

    704 Words  | 3 Pages

    Lab Report 5: Acetylsalicylic Acid (Aspirin) Synthesis Name: Divya Mehta Student #: 139006548 Date Conducted: November 19th 2014 Date Submitted: November 26th 2014 Partner’s Name: Kirsten Matthews Lab Section: Wednesday 2:30 L9 IAs Name: Brittany Doerr Procedure: For the procedure, see lab manual (CH110 Lab Manual, Fall 2014) pages 96-98. Wilfrid Laurier University Chemistry Department. Fall 2014. Acetylsalicylic Acid (Aspirin) Synthesis.

    Read More

  • Enzyme Fast Lab

    485 Words  | 2 Pages

    purpose the propose of this experiment was too see if the chemical reaction of a enzyme can be made faster. Hypothesis I think that a warm environment would be best to make an enzyme’s reaction faster. because a protein can move faster in heat.

    Read More

  • Chili Peppers In Aztec And Mayan Society

    387 Words  | 2 Pages

    intestines. Like the cayenne pepper, chilies also contain capsaicin. However, the indigenous use of chili was slightly different from that of the cayenne use. Chili peppers were used in anything and everything edible in Aztec and Mayan society. The indigenous medical uses were a gruel made of corn and chili pepper.

    Read More

  • Bio Chem: Lab Report Of Amylase In Enzyme

    916 Words  | 4 Pages

    Bio Chem lab Report 04 Enzyme Biochemistry Group Member: Chan Man Jeun Duncan (16002621) Law Sze Man (16000478) Introduction Enzyme is a protein base structure substance in our body. It works at a biocatalyst that will catalyzing the chemical reaction, which helps to speed up the chemical reaction. Enzyme could only function in specific shape, and the shape of enzyme is depending on the environment, therefore it is hard for an enzyme to function well in an extreme environment. The aim of this experiment is to see can the enzyme functions normally in different environment(pH, temperature and salt concentration) via using starch solution, amylase from saliva, 0.5M HCl solution, 0.5M NaOH solution and NaCl solution, and using iodine solution

    Read More

  • Effect Of Temperature On Amylase

    1167 Words  | 5 Pages

    5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).

    Read More

  • How Does Acid Rain Affect Plant Growth

    1186 Words  | 5 Pages

     Two of the same types of plants were used, in order for the results to be reliable.  One of the plants was labelled DISTILLED whereas the other was ACIDIC, so the results don’t get mixed.  The DISTILLED plant was used as a controlled variable, where normal spring water was used (pH 7.3)  The ACIDIC plant was the one the experiment had taken place on, this was where lemon water was used (pH 2.0)  Throughout the experiment pictures were taken, so one will be able to notice the colour change on the acidic plants, as it had started to slowly die. With both plants you are able to notice that they had both started to grow flowers, but more the spring watered plant than that on the lemon juice watered one.  The results of the plant growth was recorded to we would be able to notice the amount it had grown, this would be the water compared to lemon juice.

    Read More

  • Lab Report On Catalase And Temperature

    899 Words  | 4 Pages

    H20 + 2 O2 This experiment will use 1% catalase solution and 3% hydrogen peroxide solution, both diluted into water so the reaction slows down. Temperature will be controlled in this experiment to change the reaction speed of the enzyme and the substrate, this is what the experiment is looking at. The effect of the temperature will be determined by how much gas is released in two minutes, which will change the pressure inside the test tube and will be measured by a gas

    Read More

  • Effect Of Enzyme Concentration On Enzyme Reaction

    1046 Words  | 5 Pages

    ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.

    Read More

  • Aspirin Lab Report

    1524 Words  | 7 Pages

    The hydrolysis formed salicylic concentration which was mixed with iron(III) solution to form a purple complex. This was then use to study under the UV/Visible absorption spectroscopy which gave absorbance values recorded at 525nm to determine the concentration of salicylic acid using the Beer Lambert’s Law and later corrected to find the actual concentrations. The concentrations of aspirin at various intervals was found from salicylic concentrations. Upon plotting a graph of ln(aspirin) vs time, it produced a linear equation from which the gradient gave the rate constant of 0.0083min-1 and the overall shape of the graph concluding this reaction to be pseudo first order with respect to the concentration of aspirin with the deviations and improvements as

    Read More

  • Hydrochloric Acid And Sodium Thiosulphate Lab Report

    1539 Words  | 7 Pages

    Introduction The goal of the experiment is to examine how the rate of reaction between Hydrochloric acid and Sodium thiosulphate is affected by altering the concentrations. The concentration of Sodium thiosulfate will be altered by adding deionised water and decreasing the amount of Sodium thiosulphate. Once the Sodium thiosulphate has been tested several times. The effect of concentration on the rate of reaction can be examined in this experiment.

    Read More

More about Amide Bond In Capsaicin

Open Document