2 drops of phenolphthalein is added to the solution by using dropper. The colour of the solution change to light red. 4. Burette is filled with sulphuric acid to titrate the solution with drop by drop while the conical flask is shaken slowly until the light red colour disappear. 5.
Tests for alkaloids a. Morquies test: For detecting the alkaloids 2-3 gms of the sample was ground with sufficient chloroform to make slurry. Ammonical chloroform was added and the mixture was stirred for one minute. Extraction of alkaloids from choloroform was accomplished by shaking the solution with 0.5 ml of 2 N-H2SO4 and separation of the acid layer by means of a dropper. A few drops of drug solution were tested with the following alkaloidal reagents A small quantity of the drug solution was placed in a glass plate and allowed to evaporate to dryness. A drop of water and Morquies reagent (HgCl2 + KCN) was added and the colour was observed.
Two drops of iodine- potassium was then added to the mixture. The procedure was repeated with the rest of the samples and isopropyl with varying drops of sodium hydroxide. Eight drops of sodium hydroxide was added to formaldehyde, 9 drops to cyclohexanone, 15 drops to benzaldehyde, and 7 drops to isopropyl. It was observed that both cyclohexanone and benzaldehyde formed a yellow layer that lasted only for a few seconds before disappearing completely while the formaldehyde mixture produce a yellow precipitate. Both acetone and isopropyl mixtures were heated since no precipitate was observed.
The reaction mixture was then merged at a Y-piece with a reagent stream consisting of potassium ferrocyanide and luminol in alkaline solution. Detection: The elicited chemiluminescence intensity was measured by a photomultiplier tube operated at a voltage of 880 V. Range: 0.1–14 µg mL-1 8. “TLC for Neomycin Sulphate”  Stationary Phase: silica gel Mobile Phase: 3%ammonium:Acetone
Subsequently, monochloroacetic acid (60 g) was added, in five equal portions, at 1 min intervals. Heat was then applied to bring the reaction mixture to a temperature of 60 oC and stirring at this temperature was continued for 3 h. Afterward, the reaction mixture was filtered and the filtered solid product (CMCS) was thoroughly rinsed with methanol. The resultant CMCS was dried in an oven at 60 oC. The Mw of the produced
1- Extraction method No. 1: Fifty grams of powdered aerial parts of portulaca oleracea were hydrolyzed by using reflux for 9 hr. with 300 ml of 2N hydrochloric acid then the extract cool at room temperature ,filter and wash the residue with 2N of ammonia solution. The residue dried overnight at 60ºC ,the final step involve the extraction of the residue with 250 ml of chloroform by using soxhlet ,the final extract cool at room temperature ,then filter and evaporate to dryness by using rotatory evaporator at 40ºC to yield (2.264 gm),as show in scheme (2-1). 188.8.131.52-Extraction method No.2: Fifty grams of powdered aerial parts of portulaca oleracea extracted by soxhlet with 500ml 0f 70% ethanol for 8 hr.
The purpose of this experiment was to perform a Wittig reaction using two different methods: In method I, 250 mg aldehyde was mixed with 785 mg phosphonium salt in 5 M NaOH solvent. This mixture was stirred for thirty minutes and filter by vacuum filtration for the product. In method 2, 250 mg of aldehyde, 785 mg, benzyltriphenylphosphonium chloride, and 380 mg potassium phosphate tribasic were homogenize with a pestle and mortar. Vacuum filtration was also used in this method to attain the product. The products in both methods were used for recrystallization and TLC.
water was added to the drug, polymer, and MCC mixture and mixes it thoroughly to form a wet mass. It was sieved through sieve no.18 to form granules.Then the granules were collected and dried in an hot air oven at 60◦C for 20 min.The dried granules were passed through sieve no.30. Then the dried granules were pre lubricated with Aerosil and talc and finally lubricated with magnesium stearate. The tablets were compressed using 17.5X7.5 mm oval shaped punch.Good physicochem- ical properties were observed for of the prepared matrix tablets. The weight variation was observed within the range (650 mg).
The aqueous extract was prepared by dissolving 1g of dry extract with 20 ml of sterilized distilled water, so the final concentration of extract would be 0.05 g/ml, from this solution other concentration were prepared (0.1-0.2) g/ml. the solutions were shaken for 30 min. The extract was centrifuged (30,000 rpm; 15 min) and the supernatant was Separated. To hydroalcoholic extract, 80 g of the powder was extracted with aqueous methanol (75%). The other two concentrations were prepared from soaking sixfold aqueous methanol (75%) with different amounts of powder.
petroleum ether, chloroform, methanol, n-butanol, ethyl acetate and water. After solvent extraction, it was evaporated to obtain a powdered extract for various biochemical analysis. Preliminary phytochemical screening of the extracts was performed for the presence of alkaloids, flavonoids, steroids, tannins, saponin, phenol and by the standard procedures. Alkaloids: To 1 ml of extract, 2-3 drops of Wagner’s reagent were