Carbon Balance Analysis Essay

1.12.4 The carbon balance analysis

The carbon balance analysis would determine the carbon source transformation along the biofiltration process. The analysis provided details how toluene carbon distributed into products of biomass accumulation in the filter bed, CO2 or mineralization, and leachate partition during the biofilter process.

Regarding to calculation for carbon weight, daily toluene removal rate, wet biomass, CO2 generated concentration, and TC in leachate were performed through following responsible equations in Table 2.2 below.

Where ∆toluene is toluene removal rate, mg/m3, Wb is wet biomass, g, ∆CO2 is CO2 concentration, ppm, XTC is total carbon in leachate, g/L, Air flow rate, L/min, V is the volume of filter bed, L; λ is the ratio of dry biofilm weight to wet biofilm weight, dimensionless, where 0.11value was used in this study, time is 24, hours, MCO2 is molecular weight of CO2, g, Vgas is gas volume of 1 mole of

These slides were in order to grow biofilm for 2 weeks. Also, another reactor biofilter system, called BF3, was set up provided with all the air feeding line connection with air pump, customized toluene generation bottle, UV lamp, and spray unit.

After 2 weeks of growing biofilms, glass slides were retrieved from both biofilters and placed inside BF3. Then, the ozone concentration was varied between 200-800 mg/m3, and those slides were treated with varied period was between 5 min to 24 hours. Beyond the treatment, the biofilms were stained with ViaGram. Red + Bacterial Gram Stain and Viability Kit and observed by the confocal laser scanning microscope following the earlier procedure. Lastly, the 3D CLSM images was rendered using the same parameters in Imaris 8.0.2 software as mentioned