Add deionized water to the volumetric flask to the 250ml mark on the volumetric flask. 13. Read the volume from the bottom of the meniscus. 14. Swirl the solution to ensure that the oxalic acid crystals are properly dissolved in the deionised water.
In the round-bottom flask (100 mL), we placed p-aminobenzoic acid (1.2 g) and ethanol (12 mL). We swirled the mixture until the solid dissolved completely. We used Pasteur pipet to add concentrated sulfuric acid (1.0 mL) to the flask. We added boiling stone and assembled the reflux. Then, we did reflux for 75 minutes.
5 mL of 3M sodium hydroxide, 5 mL of de-ionized water, and 15 mL of hexane were added to the reaction flask and stirred. The mixture was transferred to a separatory funnel, separated into an organic layer and water layer, and then drained. The water layer was washed twice with 10 mL of hexane. The organic layer was dried
Eventually using the NaOH and the acid’s consumed moles, the equivalent mass will be determined. Procedure: Part 2: Obtain 45mL of NaOH, and then weigh 0.3-0.4g of the unknown acid (KH2PO4). Dissolve the acid into 20.00mL water. Record the buret readings, and slowly titrate the NaOH into
The major bands from the IR are, FTIR: sp3 O-H stretch, about 3200-2500 cm-1 (b, m); sp3 C-H stretch, 2991-2868 cm-1 (sh, m); sp2 =C-H stretch, 3100-3000 cm-1 (sh, w); sp2 C=O stretch, 1701 cm-1 (sh, s); and sp2-sp2, aromatic, C=C stretch (in ring), 1507 cm-1 (s, m). Formation of Diastereomeric Salts: 30 mL of 0.25 M sodium hydroxide was added to the flask that contained the crude ibuprofen. This flask was heated to 85°C with the addition of 0.9 mL of S-(-)-α-phenethylamine (d=0.94 g/mL, 6.981 mmol). The crude salt was collected via vacuum filtration for 30 minutes and the percent yield of the salt formation was 98.688 %.
MDA level was determined by thiobarbituric acid reactive substances (TBARS) in serum, based on the reaction between MDA and TBARS. Standard Malondialdehyde solution in 5 mL of volume was processed along with test samples. 1.5 mL of 0.8% of TBA was added to 1 mL of the serum sample. Then 0.4 mL of 8.1% sodium dodecyl sulphate and 1.5 mL of acetic acid was added. The mixture was finally made upto 5 mL with distilled water and placed in hot water bath at 95ºC for 1 h. After cooling, 1 mL of distilled water and 5 mL of the mixture of n-butanol and pyridine (15:1, v/v) was added.
Dissolve the salt in 120 ml of tap water. Add 60 ml 6 M Hcl and stir the mixture with a glass rod. Add 24 g solid Nacl to the solution and stir the mixture for about 2 minutes. Support a 500 ml separatory funnel on a ring, close stopcock and then a clean beaker is placed beneath the exit tube. Transfer the aqueous solution from the beaker to the separatory funnel.
2ml of 10% ammonium citrate was added to each beaker. The pH was then adjusted to 8.5 by adding 10 drops of 5M NH4OH(aq) to each beaker. 3ml of 0.1% cuprizone was added to each beaker. The four solutions were then transferred to 25ml volumetric flasks. The beakers were washed with de-ionized water, and the washings were combined with the solutions in the 25ml volumetric flask.
Standard Preparation: 100 mg of standard ascorbic acid was weighed precisely and transferred to a 100 ml volumetric flask, added 70 ml of 0.5% sodium metabisulphite and dissolved by shaking. The volume was made up to the mark with 0.5% sodium metabisulphite for getting a concentration of 1 mg/ml. 2 ml of this solution was taken into another 100 ml volumetric flask and made the volume up to the mark with 0.5% sodium metabisulphite which resulted in concentration of 0.02 mg/ml. The solution was filtered through 0.45 µ nylon syringe filter. Sample Preparation: 2.5 g of sample was weighed accurately and transferred to a 100 ml volumetric flask.
• Iodine Solution Weigh 7.7g of potassium iodide into a 50cm3 beaker. Use distilled water to help the iodide dissolve. Swirl for a few minutes until the iodide has completely dissolved. Using a funnel to help, pour the potassium iodide into a 500cm3 volumetric flask, make sure all traces of the solution is in the volumetric flask. Using distilled water would be a good method in order to rinse the beaker.
Then, the pipet was rinsed with distilled water. The bulbs were then attached to the pipette; filling and dispensing water were practiced using both bulbs. Furthermore, the 250-mL beaker was weighed, and its mass was recorded. After that, the Erlenmeyer flask was filled with 100 mL of distilled water. The temperature was recorded.
The silver ion TLC was prepared through the following procedure: Silver nitrate was dissolved in 10 ml of distilled water. This aqueous solution of silver nitrate was absolutely mixed with 9 g of silica gel (10 ~ 40 μm particles). Then, a 10 × 5 cm TLC plate was coated with the above slurry and activated for 1 h at 90 °C before use. They were immediately transferred into a desiccator in dark for storage after cooling. 32 100 μL of afore-prepared sample solution and the mixed reference standard were diluted 100 times with ethyl acetate.
The second test consisted on mixing in a 100ml beaker, 30ml of water and the respective amount used in the previous step of each element and analysing what the reaction was. The third test was in a 125ml Erlenmeyer flask and we mixed 30ml of M Hydrocloric Acid and 7g/4g of