Cardaria Draba Research Paper

Determination of phytochemical composition and ten elements content (Cd, Ca, Cr, Co, Fe, Pb, Mg, Mn, Ni and Zn) of Cardaria draba by GC-MS, FT-IR and AAS techniques

Haider Mashkoor Hussein

College of Science, Al-Qadisiya University, Iraq

ABSTRACT

The research objectives were to investigate the phytochemical composition of the methanolic leaves extract of Cardaria draba and its elements content. GC-MS analysis revealed the existence of the 8-Hydroxy-2-octanone, Phosphorothioic acid , S-ester with trimethylenediiminodipropa, Pyrrolidine , 1,1'-methylenebis, N-[4-Aminobutyl]aziridine, Cycloheptane , 1,2-dimethoxy-,trans, N-[3-[N-Aziridyl]propylidene]tetrahydrofurfuralamine, o-Cymene, 1,13-tetradecadien-3-one, 5-Octadecenal, 2,6-Nonadienal

draba were dried at room temperature, and the plant powderd was reduced in size with a sieves. About 20 grams of sample powders were soluble in 2oo cm3 methanolic extracts. The filtrates (crude extract of C. draba) obtained were concentrated in rotary evaporator keeping the water bath at 50 Cº. Then all the extracts were preserved in separate containers at 5 Cº for further experimentations.

Calibration curves and elementary analysis (heavy metals) of C. draba samples:

Calibration curves were prepared using five concentrations, and the linear correlation coefficients had been determined. Know weight of the C. draba leaves was mixed with few drops of Conc. trioxonitriate acid until a slurry is formed. Concentration of elements present were determined using atomic absorption spectrophotometer (WHO, 1998).

Gas chromatography – Mass Spectrum

Latterly the acceptable of traditional medicine as an sussesion form of health maintains and the development of mycobacterial resistance to any antibiotics has tend authors to determinate the antimicrobial viability of the parts medicinal plants36-38 . GC-MS analysis of plants compounds was supply in methanol leaves extraction of Cardaria draba, appear in Table (1). The chromatogram GC-MS of the 46 major peak of the sampls compounds determinates was results in Figure (1). Chromatogram GC-MS analysis of the methanol extract of Cardaria draba showed the presence of forty six peaks and the constitutes depending to the peaks were estimates as following. The next peaks considered to be, 8-Hydroxy-2-octanone, Phosphorothioic acid , S-ester, Pyrrolidine , 1,1'-methylenebis, N-[4-Aminobutyl]aziridine, Cycloheptane , 1,2-dimethoxy-,trans, N-[3-[N-Aziridyl]propylidene] tetrahydrofurfuralamine, o-Cymene, 1,13-tetradecadien-3-one, 5-Octadecenal, 2,6-Nonadienal , (E,Z), Piperidine , 1-(1-propenyl), Imidazole , 2-amino-5-[(2-carboxy)vinyl], S-Benzyl-l-cysteinyl-S-tetrahydropyranyl-l-cysteinyl hydrazide, β-D-Glucopyranose , 1-thio-,1-[N-hydroxy-5-(methylthio)pentar, 9-Oxabicyclo[3.3.1]nonane-2,6-diol, Formamide