Fluorescence Spectra Lab Report

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3. RESULTS AND DISCUSSION The studied anthlementic drug contain secondary amino close to a carbonyl group which enable the drug molecule to form a complex with metal ions. 3.1. Fluorescence spectra The studied drug have a very weak native fluorescence in aqueous solution. However, it was found that upon its complexation with lanthanum ions, its fluorescence intensity was greatly enhanced. The stability of the complex was improved in the presence of phosphate buffer (pH 5.5). An illustration for the fluorescence spectra for MBZ in presence and absence of the metal are shown in Fig. 2. 3.2. Optimization of reaction conditions The influence of the different experimental parameters on the complex formation were carefully studied and optimized. Each factor was changed individually, keeping the others constant. The factors included effect of pH, buffer volume, type, concentration of La (III), type of surfactants,…show more content…
The fluorescence intensity of the formed metal chelates was directly proportional to the drug concentration in the linear range of 0.07 – 0.80 µg ml-1 of MBZ. The method have very high linearity as the calculated correlation coefficients (>0.9996) were very close to 1. Other analytical parameters of proposed method are presented in (Table 2). 3.5.2. Detection and quantitation limits Sensitivity of the developed method was evaluated by calculating the detection and quantitation limits. These limits were calculated using the formula; LOD=3.3σ/S and LOQ=10σ/S for limits of detection and quantitation, respectively, where σ is the standard deviation of intercept and S is the slope of calibration curve. The obtained LOD and LOQ values were 0.022 and 0.068 μg mL-1, respectively (Table 2). These values indicates that the high sensitivity of the developed method as it can quantify a concentration of the studied drug down to the nano-gram level. 3.5.3.

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