The three things that can cause the enzyme to denature is a large change in pH level, High Temperature, and substrate concentration. According to our knowledge, we know that a large change in pH will cause instability in the protein structure thus resulting in denaturation of the enzyme. From the data, we can see that pH 3 (total:6.3) and 10 (total:6.2) were the slowest because pH 3 is probably the highest acid and pH 10 is the highest base. The highest acid or base pH represents a large change which would cause the enzyme to denature. The fastest pH was 6 (total:34.5), and it seems that there wasn’t a large change which resulted in a stable structure.
7. In this experiment, if the sucrose concentration were increased to 70 g/l would you expect sucrase activity to be significantly higher than the activity at 35 g/l. Explain your answer. No, because based on the results once it reached 30 g/l 35 g/l the results had stayed the same. There, the activity is lessening and coming to what looks like a plateau.
The competitive inhibitor that was added was lactose. We predicted this because competitive inhibitors block and bind to the active site so it will slow down the binding of the desired substrate. An alternative hypothesis that came up was that the reaction of substrate would stay consistent as if no inhibitor was added. The enzyme could reject the inhibitor if it does not fit in the active site, causing the substrate to bind as it normally would. Our results showed that with the addition of lactose, the reaction did slow down a considerably
While, Full Throttle finished in first, due to its percentage increase of over 20.60%. But why is this? Was it due to the mouse, since RockStar was tested by Mouse #2, compared to Full Throttle being tested with Mouse #3? All things considered, it probably was due to the sodium content. As mentioned earlier, Full Throttle finished first, due to it having the most distance being ran, however, it was second in sugar content compared to Monster, however the decrease was quite minimal as it was only a 4 gram difference.
They are proteins that are complexly folded to allow smaller molecules to fit into them; this active site is where substrate molecules bind. Enzymes must collide with one another at a precise position with enough activation energy. The active site must bind to the reacting molecule, or the substrate (1). Enzyme-catalyzed reactions require lower activation energy. The activity of an enzyme is affected by its environmental factors, and any change results in an alteration in the rate of the reaction caused by the enzyme (2).
At the level of the Krebs cycle, it primarily inhibits enzymes that require lipoic acid as a cofactor, such as pyruvate and alpha-ketogluterate dehydrogenase. This then inhibits the Krebs Cycle, which stops the production of reduced NAD (NADH). Therefore, arsenic poisoning kills by allosteric inhibition of essential metabolic enzymes, leading to death from multi-organ
In comparison to the average pH of the distilled water, the average pH of the HCl solution has decreased the most out of the solutions tested, which proves that an increase in this acid in the body raises the pH of the blood. In order to determine whether excessive crying increases or decreases the pH of the blood, one needs to analyse the readings of the solution containing the dry ice This solution will give an accurate conclusion because when one cries excessively, the person tends to hyperventilate which causes a lack of carbon dioxide in the body. Due to the lack of carbon dioxide in the body, it opposes the results obtained, which shows that excessive crying increases the pH of the blood and therefore causes alkalosis. Similarly, severe asthma causes shortness of breath which in theory decreases the CO2 in the body. Looking at the results as well as the findings for the mechanism of severe crying, one can see that the shortness of breath cause by severe asthma causes alkalosis, which is when the pH of the blood is too
Assess your progress number 15 Describe the functions of proteins in the body. The body uses essential and nonessential amino acids to synthesize proteins. Proteins perform numerous functions in human body, like collagen provides structural strength in connective tissue, as keratin in the skin, and the combination of actin and myosin makes muscle contraction possible. Enzymes regulate the rate of chemical reactions, and protein hormones regulate many physiological processes. Proteins in the blood prevent changes in pH promote coagulation factors, and transport oxygen and carbon dioxide.
a. What are Enzymes Enzymes are very efficient protein based catalysts for biochemical reactions, which are essential to all living this to sustain life. Enzymes itself are not alive as they are proteins, however they are still made by living things and act as a catalyst to speed up the overall chemical reaction, asmost chemical reactions in biological cells would occur too slowly if it was not for these enzymes. Despite them making chemical reactions move quicker, they are not changed by the reaction. b. Optimal Enzyme Temperature There is a certain temperature at which an enzyme's catalytic activity works at its best and is at its greatest.
However, the improvements were highly statistically significant in group B as compared to group A. This marked reduction in the proportions of sites that bled on probing represents significant reduction in inflammation and possibly, bacterial load in these sites. This is in agreement to another study by Gonzales et al17 where in the mean percentage of bleeding sites decreased from 95% to 35% after 10 days and to 42% after 28 days of placement of CHX chip. Goodson et al.18 have shown that local delivery of antimicrobial drugs had a significant effect in reducing the red-complex bacteria in the periodontal pocket. The larger reduction in PBI observed in the present study is likely due to the anti-infective effect that the frequent applications of these chips had on the microbial flora with chlorhexidine and inflammatory response due to flurbiprofen.