ipl-logo

Catalase Enzyme Lab Report

461 Words2 Pages

1. What temperature will have the fastest reaction if a catalase enzyme and hydrogen peroxide are mixed together?
2. If catalase enzymes and hydrogen peroxide are mixed together, then the fastest reaction will occur when the test tube is placed in a 30 degrees C water bath because that is the temperature when and hydrogen peroxide react the fastest.
3. Amount of catalase enzyme added
b. 0.5 cm3
c. cm3
d. 10 cm3 test tubes. The catalase enzyme was added to each test tube, so the reaction was possible.
4. Height of Oxygen Bubbles
b. cm
c. 10 cm3 test tubes, 0.5 cm3 catalase enzyme, 4 cm3 of H2O2, and Certain Temperature (10 degrees C, 20 degrees C, 30 degrees C, 60 degrees C) The test tube is where the dependent variable that is measured. …show more content…

Exergonic Reaction with Catalyst

First, pour about 15 ml of substrate solution into a 25 ml beaker. Then, put the substance in an hot water bath and set it to 30‹C. Next, add a filter paper disk soaked in yeast solution and record the time it takes for the disk to rise to the top of the solution. Repeat steps 2 and 3 2 more times for 30 ‹C and 3 times for 40‹C. Finally place the solution in a ice bath for 20‹C and repeat steps 2 and 3 3 more times. First, pour about 15 ml of yeast solution into a 25 ml beaker. Then, add a filter paper disk soaked only in water in the substrate solution and wait until it sinks to the bottom. Next, add a filter paper disk soaked in substrate solution and it should immediately sink to the bottom. After, record the time it takes for the disk soaked in the substrate solution to rise to the top of the solution. Repeat the 3rd and 4th step 2 more times.
References:
• (2010, December 3). Structural Biochemistry/ Enzyme/ Gibbs free energy graph. Online Image. Retrieved October 22, 2015 from

Show More
Related

  • Copper Transformation Lab Report

    1931 Words  | 8 Pages

    Copper Transformations Prelab Questions Three metals ions are Magnesium, Iron, and Nickel. Iron is used in the sea with iron rich minerals, for substances. Iron was also used in the formation of earth.

    Read More

  • Enzyme Lab

    901 Words  | 4 Pages

    Using two test tubes, label one “s” for substrate and the other “e” for enzyme. The substrate tube should contain 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL guaiacol and the enzyme tube should contain 6 mL of distilled water and 1.5 mL of peroxidase. Combine the materials of the substrate and enzyme tubes, mix the two using a clean transfer pipette, transfer a portion into a cuvette so that the cuvette is about half-full then cover the top of the cuvette with Parafilm and then place it in the spectrophotometer and record absorbance. Remove the cuvette and repeat recording absorbance at 1, 2, 3, and 4 minutes. Be sure to mix the cuvette and clean its surface with Kimwipes before each reading.

    Read More

  • Horseradish Peroxidase Lab Report

    1090 Words  | 5 Pages

    The products are released from the enzyme surface to regenerate the enzyme for another reaction cycle. The active site has a unique geometric shape that is complementary to the shape of a substrate molecule, similar to the fit of puzzle pieces.

    Read More

  • Catechol Oxidase Lab Report

    642 Words  | 3 Pages

    There are few vegetables and fruits that turns to the color brown if their surface is exposed to oxygen. Once the veggies or fruits been exposed to oxygen, then the browning begins to appear, and electrons and hydrogen will be removed. This happens because of an enzyme called catechol oxidase. The enzyme will act on its substrate catechol to form a yellow compound which then reacts with the oxygen in the air and change into benzoquinone. The more concentration of the enzyme, the more browning appears.

    Read More

  • Enzyme Turnip Peroxidase Lab Report

    1117 Words  | 5 Pages

    1 “substrate” and another “ enzyme.” Instead of using the distilled water, this time you are going to use different pH buffer in the enzyme test tube. In the substrate tube, add 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL of guaiacol for a total volume of 7.5 mL. For the enzyme tube, instead of distilled water add the pH solution (3) and 1.5 mL of peroxidase which equals a total volume of 7.5 mL. Use the dH2O syringe for our pH solution. To clean the syringe, flush it by drawing 6 mL of distilled water.

    Read More

  • How Fast Does Alka Seltzer Make Gas

    621 Words  | 3 Pages

    Then the scientist will observe the different rates of reaction with temperature. The Boltzmann distribution of law, indicates that high temperature makes molecules gain high energy contents (pubs.acs.org/doi/abs/10.1021/ja). In order to measure the reaction rate, the scientists must use the same volume of water at three different starting temperatures: hot tap

    Read More

  • Lab Report On Enzyme Activity

    1419 Words  | 6 Pages

    LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.

    Read More

  • Laboratory Report on Enzyme Activity

    1407 Words  | 6 Pages

    LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.

    Read More

  • The Biological Temperature Of Catalase

    750 Words  | 3 Pages

    Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).

    Read More

  • Potato Enzyme Lab Report

    1182 Words  | 5 Pages

    A scale of zero to five was used to describe the reactions, with zero being no reaction at all, one being a slow reaction, and five being a very fast reaction. The materials used were a test tube rack, six test tubes, a test tube clamp, forceps, a graduated cylinder, four small pieces of liver, one piece of potato, one piece of hamburger meat, approximately forty milliliters of hydrogen peroxide in a forty milliliter beaker, a splint, and matches. An ice bath and boiling water was required for testing, where a hot plate was used to boil the water. Each test tube given a label, which were “cold”, “room”, “hot”, “warm”, “potato”, “meat”, and

    Read More

  • Bio Chem: Lab Report Of Amylase In Enzyme

    916 Words  | 4 Pages

    Bio Chem lab Report 04 Enzyme Biochemistry Group Member: Chan Man Jeun Duncan (16002621) Law Sze Man (16000478) Introduction Enzyme is a protein base structure substance in our body. It works at a biocatalyst that will catalyzing the chemical reaction, which helps to speed up the chemical reaction. Enzyme could only function in specific shape, and the shape of enzyme is depending on the environment, therefore it is hard for an enzyme to function well in an extreme environment. The aim of this experiment is to see can the enzyme functions normally in different environment(pH, temperature and salt concentration) via using starch solution, amylase from saliva, 0.5M HCl solution, 0.5M NaOH solution and NaCl solution, and using iodine solution

    Read More

  • Effect Of Temperature On Amylase

    1167 Words  | 5 Pages

    5 water bath were set up each to10 °C. (5 were used do the experiment faster) 5 cm3 of starch solution were added into the 5 test tubes that were labeled test tubes. Then 5 cm3 of amylase enzyme was added into the other 5 test tubes that were labeled. Put one of the starch solution test tube (preferably the one labeled 1) and one of the test tube containing amylase into the water bath (10 °C).

    Read More

  • Alkaline Phosphatase Enzyme Lab Report

    2575 Words  | 11 Pages

    Predict/ roughly determine the Vmax and ½ Vmax values from the peak of the graph, where the slope of the graph levels off (the asymptotical line). Predict/ roughly determine the Km by reading off of the graph the corresponding substrate concentration on the x-axis for the ½ Vmax value. Plot a Lineweaver-Burke graph (the inverse of the velocity of the reaction vs. the inverse of the substrate concentration). Calculate accurate Vmax and Km values using the following equation for the Lineweaver-Burk

    Read More

  • Cellular Respiration In Yeast Lab Report

    780 Words  | 4 Pages

    The Effect of Sugar Concentration on CO2 Production by Cellular Respiration in Yeast Introduction In this lab, our main focus was to find how sugar concentration affect yeast respiration rates. This was to simulate the process of cellular respiration. Cellular respiration is the process that cells use to transfer energy from the organic molecules in food to ATP (Adenosine Tri-Phosphate). Glucose, CO2, and yeast (used as a catalyst in this experiment) are a few of the many vital components that contribute to cellular respiration.

    Read More

  • Effect Of Enzyme Concentration On Enzyme Reaction

    1046 Words  | 5 Pages

    ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.

    Read More

More about Catalase Enzyme Lab Report

Open Document