Catalase Enzyme Lab Report

Enzymes are biological catalysts made of proteins that accelerate chemical reactions by lowering their activation energy therefore increasing the activity rate of the enzyme and more substrates turned into products. The ‘Catalase’ enzyme that was used during this experiment was obtained from peroxisome found in celery which are organelles found in bacteria, plant, and animal cells. It is involved in the breaking down of certain substances and the diminish of reactive oxygen species and that includes hydrogen peroxide (H2O2) which can be a byproduct of the metabolism of oxygen. Hydrogen peroxide is toxic to the cell and so the catalase enzyme is utilised to break down H2O2 to form oxygen molecules and water free of free radicals.

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The standard assay was prepared with a 9 pH level and the temperature chosen was 70℃ since the best results were achieved at those specifics. Three graduated cylinders were used since the best way to observe the difference in the rise of foam was to have all three treatments next to each other. The first graduated cylinder was labeled ‘C’ since it was the control group which had no treatment applied to it. The second graduated cylinder was not labeled and that indicated it was the group with the heat treated catalase. The 5 mL of catalase was put in the 90℃ water bath for five minutes and was then put in an ice bath after the five minutes were up. Lastly, the third graduated cylinder was labeled ‘V’ since it contained 440g of ascorbic acid also known as vitamin C. All three graduated cylinders were put in the 70℃ water bath after adding their own catalase alongside three test tubes of H2O2 for 30 seconds. After the specified period of time, the graduated cylinders were taken out of the bath and the H2O2 was added to each of them and that is when the timer started. The recordings were taken every 30 seconds for 90