The aim of the investigation was to determine the effect of pH on the rate at which catalase decomposes hydrogen peroxide and consequently answer the researchable question “How does pH influence cells and consequently an organism”. The hypothesis, “As the pH deviates from 7 the initial rate of oxygen production will decrease” is supported by the results. The trend displayed in Figure 3 is, as the pH deviates from 7 the initial rate of reaction decreases. Figure 3 shows that the rate of reaction (%O2/s)
Catalase Experiment Can hydrogen peroxide be broken down by catalyst other than those found in a living system? Independent and Dependent Variables Independent Variable: The independent variable is the chicken liver and the potato. Dependent Variable: The dependent variable is what I will be measuring which is how much peroxide I will use for each individual experiment. I will also need to measure the temperature of the peroxide during/after the experiment and the pH balance. Purpose “What was
3.6.4 Assay of Catalase (CAT) Catalase activity was assayed by measuring the inhibition rate of Hydrogen peroxide at 240nm according to the method described by Luck (1974). For this assay, • A 20% homogenate of the leaf extracts of different plants was prepared in phosphate buffer, 0.067 M (pH 7.0). The homogenate was then centrifuged. The supernatant was then used as enzyme extract. • Hydrogen peroxide (H2O2, 2mM) in phosphate buffer (3.0ml) was taken in an experimental cuvette, followed by the
Effect of temperature on the reaction between the catalase and H2O2 Figure 1 shows that the optimum temperature for catalase to catalyze hydrogen peroxide is around room temperature (30℃) as it has a very fast reaction rate. The overall trend is that temperatures different from 30℃, will make the reaction rate decrease. Discussion This experiment supported the hypothesis, since catalase was the most effective with hydrogen peroxide when it was in an environment with
Catalase and Temperature Introduction Background: Enzymes are catalysts which help reactions inside of organisms such as cells. Many different types of enzymes are used to catalyze different types of reactions. Enzymes are able to catalyze reactions that normally wouldn’t be possible under the specific circumstances in the cell such as the pressure or temperature of the cell. The way an enzyme works is it binds with the active site of a substrate and creates an enzyme substrate complex. The enzyme
catalyzed reaction, as mentioned previously. In the first part we observe a normal catalase reaction and rate its reaction rate. In the second part we learned which tissues, of the ones tested, contained catalase. In the third part we saw how temperature can leave an effect on catalase activity. The fourth and final part was to observe the effect that pH has catalase activity. Before proceeding to begin our experiment, we gathered our materials of vinegar, baking soda, hydrogen peroxide, pH meter
agent and if too much is produced damage to the cells and some proteins can occur. Cells in the human body have an enzyme called Catalase that breaks down hydrogen peroxide into water and oxygen, in turn, preventing cellular damage. Hydrogen bonds create enzymes by the different patterns of hydrogen bonds between amino acid components. The purpose of experiment 1 was to measure the bubbles as an indirect measure of degradation. A well-developed hypothesis would be the more
enzyme activity Exploration: Introduction: Catalase is an enzyme normally found in many plant and animal tissues. Its purpose is to destroy toxic substances like hydrogen peroxide which is a byproduct in many cellular reactions. In this lab, we will use a catalase solution from yeast and determine the effect of substrate concentration on the action of this enzyme. The substrate of the enzyme will be different concentrations of hydrogen peroxide (H2O2). Catalase works by the following mechanism : 2 H2O2
preferred temperature and pH range (2). The preferred temperature for catalase falls between the ranges of thirty five to fifty degrees Celsius (4). Temperatures that are too high denature the enzyme and halt the enzyme’s activity (2). Catalase denatures starts to denature at fifty five degrees Celsius (2). Reactions in the human body produce hydrogen peroxide as a product (1). Since hydrogen peroxide is poisonous to the human body, catalase catalyzes hydrogen peroxide into water and oxygen (2 H2O2 → 2
shape of an enzyme and the substance upon which the enzyme works all have to match exactly. This enables the substrate to bind, but it can 't do this if the shapes of the two are different. The Aim of Enzyme Catalase Experiment is making a series of experiments involving the enzyme Catalase which has been performed in order to determine some of the enzyme 's properties. The enzyme found in different conditions which its specific reaction rate. Variation in enzyme concentration, variation in pH, variation