Catalase Test
Catalase Test, in this test the microbial culture from Nutrient Agar plates were used. This test determines the production of catalase by the microorganisms. Catalase is an enzyme which decomposes hydrogen peroxide to water and oxygen gas thereby, protecting the microorganisms from the lethal effect of hydrogen peroxide which is accumulated as an end product of aerobic carbohydrate metabolism. (Bahrami-Hessari et. al. 2014) The slide containing Micrococcus luteus produced bubbles when 3% hydrogen peroxide was dropped on it. (see Figure 9.3) This indicates that Micrococcus luteus produces catalase. Vigourous bubbling occurred when he slide containing the unknown microbe was dropped with 3% hydrogen peroxide. (See Figure 9.4) This indicates that the unknown microbe can produce catalase.
Nitrate Broth contains potassium nitrate as the source of nitrate. It is used to differentiate microorganisms that have the ability to reduce nitrate. Nitrate may be reduced to nitrite, ammonia, and nitrogen gas. The nitrate reduction test reagents used are, sulfanillic acid (nitrate reagent A), and
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Pyruvate can be metabolized into neutral compounds such as acetoin, 2-3 butylene glycol, and diacetyl. [2 pyruvate -> acetoin + 2CO2 ; acetoin + NADH + H+ -> 2,3-butanediol + NAD+] “In the presence of alkali (KOH) and atmospheric oxygen, acetyl methyl carbinol is oxidized to diacetyl, a reaction which is catalyzed by alpha- naphthol. Diacetyl formed reacts with guanidine-containing compounds such as arginine contributed by peptone in the medium, to form a red colored product. The resultant red color is indicative of a positive VP test.” (Pradhan, 2014) In both cultures, there is no red coloration observed (see Figure 11.6 and 11.7), thus Micrococcus luteus and the unknown microbe are both unable to produce acetoin via butanediol
Catalase Lab
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Unknown Culture Lab Report
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824 Words | 4 PagesThe compounds tested in included the unknown, Calcium Nitrate, Calcium Chloride, Calcium Carbonate, Sodium Chloride, Potassium Chloride, Magnesium Chloride, and Ammonium Chloride. The next test was the pH test. In this test, the aqueous solutions from the flame tests were used again. A piece of pH paper was dipped into the aqueous solutions, a different piece for each solution. The ensuing coloration of the paper was compared to the pH scale and the
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517 Words | 3 PagesBeing able to identify unknown microbes from systematic testing is what makes the field of microbiology so important, especially in infectious disease control. Using the testing procedure laid out by the microbiology field we are able to identify unknown bacteria present in our everyday lives, and along the way learn a lot about their characteristics that separate them from other types of bacteria. Being able to do this is vital in order for us to understand why microbes are present in certain places, how they are able to grow and what restricts their growth, that way they can be combatted if necessary. These techniques for determining unknowns are also important for isolating and testing infectious disease microbes in order to prevent spreading. Another important aspect of being able to identify unknown microbes is the
Unknown # 10 Essay
650 Words | 3 PagesStarch amylase testing was equally unsubstantial since the only amylase producing bacteria was ruled out after Gram staining. Unknown #10’s negative citrate test result was also unhelpful because E. coli is citrate negative and P. vulgaris is a variable citrate producer that can also be citrate negative. H2S production in the Kligler’s Iron Agar test ultimately proved that Unknown #10 was Proteus vulgaris. P. vulgaris is the only assigned bacteria that produces H2S, so when a black precipitate obscured the yellow butt of the Kligler’s Iron Agar slant, E. coli was ruled out. Not only did the H2S product confirmed that Unknown #10 was P. vulgaris, it confirmed P. vulgaris’ motility.
Exercise 14 Unknown Identification Lab Report
449 Words | 2 PagesSince unknown organism B was gram positive, I had decided my first biochemical test would be the Catalase test per lab manual. The result of the catalase test after adding H2O2 was immediate air bubbles. The second biochemical test performed on organism B was starch agar per lab manual. Once the reagent was added I did not
Blood Agar Lab Report
1057 Words | 5 PagesThe Another medium used was MAC, it is used to isolate and differentiate gram-negative organisms and it is a pink, dusty rose color. Lastly, the Citrate Slant is a green color and it was used as a differential test to examine enzymes. The media were inoculated at 37°C for 48 hours, then it was observed to determine the
Potato Enzyme Lab Report
1182 Words | 5 PagesIntroduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed.
Hydrochloric Acid Lab Report
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Lab Report On Fermentation
1432 Words | 6 PagesThen, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks. The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology). The tests detect the presence of carbon dioxide and ethanol. Carbon dioxide should be present irrespective of the type of respiration taking place, but ethanol is present only if fermentation has occurred. Another factor that can indicate whether fermentation occurred or cellular respiration occurred is the amount of glucose utilized during incubation.
Arginase Lab Report
1437 Words | 6 PagesThe reaction that occurs can be investigated via the adding of the liver extract which contains the arginase to urea concentrations and distilled water. The amount of urea formed is determined via spectrophotometric analysis α-INPP. The urea produced was known via the color reaction with the α-INPP, it is the reagent used for the colorimetric determination of urea. (Barry J, et al. 1984). The red color formed when the α-INPP is reacted with the urea, is sensitive to light thus it is photo labile.
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