Lab Report On Cell Culture

Introduction:

“Cell culture is a process in we culture cells in the laboratory i.e. in vitro, on a suitable medium containing essential nutrients under the controlled conditions.”

Thus, it is a process in which we grow cells artificially. There are different conditions for the different cell type. But the artificial medium as well as the substrate or medium that provides nutrient is the essential supplies. Cell cultures also require growth hormones, gases and physio-chemical regulators like pH buffer, osmotic pressure and temperature. Some cells require solid artificial media while other requires liquid culture media also known as suspension culture. There are mainly two types of culture:

a) Plant tissue culture

b) Animal tissue culture

Now-a-days,

Organotypic culture:

It is culturing of cells that retain their structure and function in the culture. In this type different cell types are used to produce a more define tissue or organ.

Histotypic culture:

This refers to the culturing of cells that will re-aggregate in to the culture and produce a three dimensional structure that is different from the tissue from which it is derive.

Primary culture:

The culture in which cells or tissues are directly taken from the organism is called primary culture.

• Cells are heterogeneous

• Slow growing

• Have same properties as of their original tissues.

Cell lines:

Cell lines are produce when primary cultures are sub-culture. There are two types of cell lines.

Continues cell line: A cell line in which there is infinite growth of cells after sub-culturing.

Finite cell line: A cell line in which cell death after sub-culturing.

Minimal requirement for animal-cell culture:

• Aseptic conditions (Clean and sterile area)

• Microbiology laboratory

• Animal house

• Facilities like equipment, liquids, chemicals and glassware

Components of media:

• Carbon source

• Phenol red dye

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