Cell Respiration Lab Research Question What is the optimal temperature for germinating pea-seeds where the rate of respiration is the greatest? Background Information Cell Respiration refers to the biochemical process conducted by the cells of an organism that combines glucose and oxygen to produce energy in the form of ATP, along with two by-products, water and carbon dioxide. The equation representing this chemical reaction is shown below. C6H12O6 + 6 O2 6 CO2 + 6 H2O + ATP Although plants and animals have different methods of obtaining glucose, the cell respiration process occurs in both types of organisms. Many external factors in the environment may affect the organism's’ rate of respiration such as the temperature of the surrounding, …show more content…
As the temperature exceeds this value, the rate of respiration will gradually decrease, reaching zero at a certain point. The optimal temperature for germinating pea seed is 20ºC because it is the average temperature of the Spring season in China, where it was first discovered. The trend for the rate of respiration is represented by the graph shown. During the cell respiration process, multiple enzymes are involved, therefore, the rate of reaction will be highest at a certain temperature, and will gradually decrease as the temperature gets further away from that particular …show more content…
As mentioned in the hypothesis, the prediction is that as the temperature increases towards the optimal, the rate of respiration will increase. As the temperature exceeds the optimal, the rate of respiration will decrease. The temperature of the environment can be varied by placing the respiration chamber under a temperature-controlled water bath/cooling bath. The temperatures that will be used in this experiment will range from 0ºC to 50ºC in 10ºC increments. Digital thermometer will be used to measure the temperature of air. Dependent Variable Rate of Respiration (Rate of which CO2 is produced in ppm/s) The rate of respiration of the germinating seeds can be represented by the rate of which CO2 is produced. The rate of respiration is expected to be affected by the change in temperature of the environment. As mentioned in the hypothesis, the change in the respiration rate is most likely to be due to the involvement of enzymes in the process. The rate of respiration of the germinating seeds can be measured by using the CO2 Gas Sensor. When connected to the data-collection interface, accurate rate of CO2 produced can be monitored. Controlled Variable Number of seeds Size of seeds Specie of
During this experiment, mitochondria were isolated from 20.2 grams of cauliflower using extraction buffer, filtration through Miracloth, and centrifusion. Twelve samples containing various volumes of mitochondrial suspension, assay buffer, DCIP, sodium azide, and citric acid cycle intermediates were prepared to be read by a spectrophotometer. The inclusion of the dye DCIP allowed for the absorbance of the reactions between the mitochondrial suspension and the TCA cycle intermediates succinate, malonate, and oxalate to be measured, as DCIP turns from blue to colorless as the activity of succinate dehydrogenase increases. Experimental Findings Increasing the number of mitochondria in the reaction did increase the reduction of DCIP relative to the amount of mitochondrial suspension present.
The data observed and recorded in this lab shows that the concentration of miracle gro’ does affect the growth rate and germination speed of black eyed peas. The data is shown through two graphs and two data tables. The control group in this experiment is the seeds with a 0% concentration of miracle gro’, therefore the seeds with just water. The experimental groups are different concentrations of miracle gro’ including a 10%, 15%, 20%, 25%, and 30% concentration. The variable in this experiment is the amount/concentration of miracle gro’.
A possible explanation for why there was no mitochondrial activity observed in the boiled corn kernels might be because increasing the temperature would disintegrate dehydrogenases and thus they wouldn't function as efficient or they
For this lab I will be using water and sucrose to demonstrate the rate of osmosis. In this lab I will be exploring how temperature impacts the rate of osmosis by placing pieces of potato of equal size in solutions of different temperatures and observing the change in mass of potato after a given period of time. The change in mass will indicate the rate of osmosis.
The control in the experiment is water. Units used while timing the productivity of gas from an Alka-Seltzer tablet in different temperatures is, seconds. In order to find out if temperature controls the rate of chemical reaction, whether hot water is a more effective way to make the gas produce at a faster speed, it would be necessary to compare the results of different temperatures at the end of each trial. In order to do this the scientists will measure the volume of gas that is produced within a 10 second interval time after the tablet begins to react.
As stated in the background of the lab, "seeds are considered to be in a period of dormancy, where they metabolize stored energy at a very slow rate while the seed is still considered alive" (Hands On Labs). During germination, the developing plant embryo uses the stored food supply in the seed for cellular energy and growth. Thus, cellular respiration is taking place as the seeds grow. Without water the seeds cannot begin germinating thus the seeds in test tube N did not undergo photosynthesis(Hands On Labs). B. Were the seeds in the “G” tube undergoing both photosynthesis and respiration, only photosynthesis, or only respiration?
This is very similar and related to the previously discussed objective of being able to identify biological processes that require energy. Modeling the way organisms capture and store free energy was achieved in several different labs, including the cellular respiration lab. In the lab, we modeled how an organism can capture and use energy through facilitating the germination of seeds. Germination is a very complex biological process that requires the capture and storage of energy for later use. Modeling this process allows further investigation and greater understanding of how energy is captured and used by
Ventilation of a person through various activities Camila Gonzalez. This lab was made with the aim of proof that making different activities can alternate the ventilation rate, also is to see the variation of work our respiration system makes. We can see the different things and situations that can affect the normal process of ventilation and respiration, like the weather and the clothes that were limitations for doing this experiment because first the weather was so hot, so the person get tired early and began to ventilate faster and second the clothes wasn't appropriate for making the activities, because they also make weight and makes that the person get more tired. We use more than one person to make a comparison of the ventilation
Cellular Respiration One of the main essentials of life that all organisms need in order to function in our world is, energy. We receive that energy from the food that we eat. Cellular respiration is the most efficient way for a cell to receive the energy stored in food. In cellular respiration, a catabolic pathway, which breaks down the molecules into smaller units, in order to produce adenosine triphosphate, also known as, ATP. ATP, is used by cells in the act of regular cellular operations, it is a “high energy” molecule.
photosynthesis and cellular respiration are extremely important in the cycle of energy to sustain life simply because nutrients would not metabolize in a productive manner. Both have numerous stages in which the process of energy occurs, and relationships with organelles located in the eukaryotic cell. Photosynthesis is a process by which green plant and other organism manufacture their food using sunlight to synthesize foods from carbon dioxide and water while cellular respiration is the oxidation of organic compounds that occurs within cells, producing energy for cellular processes. Photosynthesis occurs within organelles called chloroplasts. These organelles can absorb light, and are located inside of leaves.
The Effect of Sugar Concentration on CO2 Production by Cellular Respiration in Yeast Introduction In this lab, our main focus was to find how sugar concentration affect yeast respiration rates. This was to simulate the process of cellular respiration. Cellular respiration is the process that cells use to transfer energy from the organic molecules in food to ATP (Adenosine Tri-Phosphate). Glucose, CO2, and yeast (used as a catalyst in this experiment) are a few of the many vital components that contribute to cellular respiration.
As the temperature continues to increase, the cell parameters and volume tends to increase in a
Then, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks. The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology). The tests detect the presence of carbon dioxide and ethanol. Carbon dioxide should be present irrespective of the type of respiration taking place, but ethanol is present only if fermentation has occurred. Another factor that can indicate whether fermentation occurred or cellular respiration occurred is the amount of glucose utilized during incubation.
Research Question: How does the presence of light impact the rate of transpiration in plants? Aim: The aim of this experiment was to investigate how the presence of light affects the rate of transpiration in plants. Hypothesis: As light intensity increases, the rate of transpiration (water uptake) in a plant increases.
Germinating peas and maize respire at faster rates than fresh and dried peas and maize at room temperature and 400C(graph no). This could be because pea seeds have two cotyledons as compared to the single one of maize so oxygen usage is also more. These results support my hypothesis stated earlier. Respiration is an enzymatic process where enzymes are required at every step in the breakdown of glucose. At 600C for both peas and maize the respiration rate went down as the enzymes denatured.