3- Put the large tube in an ice bath and start to add alcohol by an increasing rate . 4- Put the tube in hot water (70º C) for 5 minutes . 5- Add 8 drops of water with constant shaking then leave the reaction to cool to room temprature. 6- Add 6 mL of ( 3 mL saturated NaCl +3 mL distilled water) i.e : half-saturated sodium chloride solution to the test tube with vigorous shaking and then wait until two layers form (upper layer is ester while lower layer which consists of a water solution of sulfuric and acetic acids ,should be discarded
Place melted agar solution and pre-warmed 2x culture medium in an ice bucket filled with hot tap water (42 °C). Also place a 50 ml conical tube in a tube holder in the ice bucket with hot water. Transfer bucket to cell culture hood for subsequent steps. 3. For the bottom layer of agar, you will need 1.5 ml of a mix of agar and medium per well of a 6-well
Experimental Procedure A dry, empty freezing-point tube and its cork were weighed together. Carefully, 15-20g of pre-cooled cyclohexane was poured into the freezing-point tube, the tube recorked, and the whole thing was reweighed, to find the exact mass of the cyclohexane. The dry thermistor and stirrer were inserted into the FP tube, ensuring they were immersed in the liquid. A large beaker was filled with ice and water, which the FP tube was placed into. The cooling curve was determined by recording the temperature at regular time intervals (every fifteen seconds) as the cyclohexane cooled, until the temperature became constant.
• Carefully decant the solvent solution from the drying agent through the funnel into the flask. Rinse the Erlenmeyer flask with about 10 ml of solvent and pour the solvent through the funnel, too. Remove the funnel, add two or three boiling chips and reattach the thermometer and adapter to the still pot. • Discard the magnesium sulfate remaining in the Erlenmeyer flask by dissolving it in tap water and pouring the solution down the drain. • Before beginning the distillation, weigh a clean, dry 1 narrow mouth screw cap bottle on a balance.
A spin vane was added and a water-jacked condenser was attached. Isopentyl nitrite (0.06ml, 0.045 mmol) was dissolved in 1,2-dimethoxyethane (0.50 ml) in a 3-ml conical vial and caped to prevent loss by evaporation. Running the reaction. The mixture in the 5-ml conical vial containing the tetraphenylcyclopentadienone and anthranilic acid was heated on an aluminum block to 140° C. Once the mixture started to boil the prepared mixture of isopentyl nitrite was added to the 5-ml conical vial through the top of the condenser using a pasture pipette. The solution continued to boil for 25 more minutes until a
During the addition of the sulphuric acid, the solution was stirred at room temperature until the amino acid (L-Phe) completely dissolved. An ice bath was prepared and used for cooling the L-Phenylalanine solution at a temperature of 40C (a selected temperature lower than 50C). Once the solution was cooled, the first portion
2.4.1. Tetramethyl glucose acetylation 1gm of tetramethyl glucose was dissolved in 5ml of acetic anhydride and added to fused sodium acetate of 0.375gm and mixed for 10 minutes and allowed to cool. To this mixture 7.5ml of toluene and 5ml of dry ether were added. The whole mixture evaporated to syrup on a water bath at 50 °C. The product dissolved in the dry ether after washing with toluene.
We added sodium carbonate until the pH of the mixture was 8. After neutralize, we collected benzocaine by vacuum filtration. We used a Buchner funnel to collect benzocaine. We used three 10 ml of water to wash the product. After the product was dry, we weighed, calculate the percent yield and determined the melting point of the product.
Commercial TiO2 P25 was obtained from Evonik. Ultrapure water (18MΩ.cm-1) was used throughout the whole experiments. 2.2. Synthesis of photocatalysts The TiO2 nanoparticles were prepared by the sol-gel method described below: 3.9 ml of TiCl4 was slowly added into 10 milliliter of absolute ethanol in reaction vesel, this reaction performed under fume hood at 0°C with vigorous stirring due to exothermic reaction,the high volatilityof TiCl4and also therelease of hydrogen chloride. Then, water was added dropwise during the mixing process.
Place the test tube of benzoic acid/lauric acid in the 60℃ water on the hot plate and when the solid solution begins to melt place the thermometer that was in the water into the test tube. Continue to heat the solution until it reaches about 55℃. Using the test tube holder, transfer the liquefied solution tube to the 25℃ water and record the temperature at 30-second intervals using a clock or stopwatch using a pencil until the solution reaches 35℃ or close to the temperature due to experimental error. While cooling, gently stir the solution using the thermometer until the solution begins to solidify. Once all the data is collected, reheat the solid solution tube in the warm water until it melts and remove the thermometer and wipe it off to avoid the solution adhering to the thermometer.