Omit any soil particles and bring the extract up to 50 ml volume with 1 N NH4OAc solution. B. Measurement According to the instructions provided operate flame photometer. Draw a calibration curve and run a series of K standards. In the soil samples measure the K by taking emission readings on the flame photometer at 767mm wavelength.
To carefully describe the relationship between the rate of reaction and the temperature, a graph of these two variables is plotted. It can clearly be seen that the rate of reaction gradually increases as the temperature is increase. This is best explained by the principles of chemical kinetics. The increase in temperature results in an increase in the kinetic energy of the molecules. Consequently, these molecules are able to move faster and the probability of collusion with other molecules is increased.
Since the value for K is always varying, therefore, it will be kept in constant to ease the burden of measuring the concentration sample. However, the absorbance of the solution cannot be measured directly. Therefore, transmittance is introduced into equation. A = log (1/T) = -log (T) A=Absorbance of the sample T= Transmittance of the
The fluorescence intensity of the formed metal chelates was directly proportional to the drug concentration in the linear range of 0.07 – 0.80 µg ml-1 of MBZ. The method have very high linearity as the calculated correlation coefficients (>0.9996) were very close to 1. Other analytical parameters of proposed method are presented in (Table 2). 3.5.2. Detection and quantitation limits Sensitivity of the developed method was evaluated by calculating the detection and quantitation limits.
From the line equation, rate was derived, rate is equal to the slope of the line. This procedure was repeated for two other solutions, generating three sets of results and three separate rates. The order of the each reactant, hydrogen peroxide and KI, was determined by creating a table that contained the volume of each reactant in each solution and the rate from the line equation. From this the
4.3. Effect of different stirring speed The Percentage extractions of the Fe3+ ion from the high-level (125ppm) aqueous solution for different stirring speed of MIBK are given in Table 3. Table 3. Percentage extraction of Fe ions at different stirring speed with MIBK. r.p.m Ce % extraction 100 13.53 95.89 200 12.58 96.18 300 4.79 98.54 400 2.95 99.10 500 11.21 96.60 As shown from table increasing the R.P.M from 100 to 400 increases % extraction then decreases; so time 400 R.P.M was chosen as optimum speed of agitation.
Constant stirring while mixing causes development of gas bubbles in the viscous preparation. So kept for 15 minute to assure the layering of gas bubbles out. 6. After that 20ml of 0.5M calcium chloride dehydrate solution is taken with accurate measurement and placed with a magnetic stirrer and rotation is maintained within 100-200rpm to prevent hindrance in the crosslinking process. 7.
o For all three trails the H202 solution in water increase by 10˚C in terms of before and after yeast is added. o The third trial has the same trend of increase as the first two but begins and ends with a 1˚C higher than the previous trials. Data processing: Number of moles for the hydrogen peroxide (H202) 34.02 = Mr Mass = 20g x 0.03 = 0.6 0.6÷34.02 = 0.017 moles Conclusion: What was learned in this lab is temperature rises when a hydrogen peroxide solution in water is activated by yeast. The hypothesis is supported by the data. Referring to what was stated, the Hydrogen peroxide solution did change based yeast that activated the solution, many were similar in temperature.
Second, 10.00 ml of the blue dye was poured into the 100.0 ml beaker and stirred for 2 – 3 seconds. The time taken by the solution to turn to colorless was measured with the aid of a stopwatch. The aim of this exercise was to determine the mixture that turned colorless in 15 minutes time. The data was recorded as shown in Table 2. Absorbance versus Time Measurements: The absorbance was set to 0 Abs while the spectrometer was set to ʎmax (from Part A).