The principle involved in this test is the precipitation of phosphate which bores a yellow-colored solution and yellow precipitate. In the sample, neither a yellow-colored solution nor a yellow precipitate appeared which indicates the absence of phosphate in the sample. In the test for Purines, or Murexide test, the standard solution used was solid guanine. The reagents used were concentrated HNO3 and 10% KOH. Positive results should be red-purple residue.
Jaspreet Singh Professor Paratore Biology 1 November 1, 2014 Spectrophotometry Identifying Solutes and Determining Their Concentration Statement of the Exercise or of the Problem The purpose of the lab experiment was to attain the following objectives: • Learning to Operate the Spectrophotometer • Construct absorption spectra for cobalt chloride and chlorophyll. Hypothesis If greater and higher concentrations of cobalt chloride are added to each solution then greater amounts of light would be absorbed by each solution. Thus a liner relationship will result in which the absorbance of a substance would be proportional to its concentration, which will be depicted, in a linear graph. Background Information: The spectrophotometer is an
KCl was discovered by dipping a wooden stick into the "Unknown 4" solution and then held in a fire. The stick with the solution let off a flame that was colored. The result was reasonable and expected because the color of the flame let off by the "Unknown 4" solution was lavender in color, which is color that is let off in the presence of KCl. These flame tests were used to identify the cations present in different solutions. Each cation gave off a very distinct color, which helped identify the presence of a cation in each solution.
It is to prevent the cell from washing away during the staining and washing process. Then, it is air dried and followed by fixing it with flame from Bunsen burner. After fixing the smear, it must be stained using Gram staining solution, firstly crystal violet solution was flood onto it, and allowed for 1 minute, then wash off with tap water. Then, flood the slide with iodine solution for 1 minute and wash it off with tap water again. The formation of a dye-iodine complex will occur in the cytoplasm.
Chromatography of Spinach Formal Discussion This lab involved the extraction of pigments from spinach leaves which were then analyzed using thin layer chromatography. The first step of this process was to grind up the leaves in order to extract the pigments. Hexanes facilitated this process and afterwards, the solution was dried over sodium sulfate to remove water. Next, the column was packed with a small piece of glass wool, followed by hexanes, and then packing sand. At this point, the column was packed half way.
Apply nail varnish to the leafs surface. 4. When the nail varnish dries, peel off the layer of nail varnish using sellotape and place it on a transparent slide. 5. Look at the layer of varnish using a magnifying glass and mark the stomata using a think marker.
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes. Chromatograms where made for the known FD&C and for the three Kool-Aid samples. The retention factor for each dye was calculated.
A blue or black tinted colour is an indicator of starch in a product, so the grey hue that the soybean milk possessed confirmed the hypothesis. Research helped to determine that the type of starch found in soybeans is known as beta-amylase (Stevenson et al., 2006). It is a starch, specified by specific glycosidic linkages, that is commonly found in the stems of plants which would lead to the conclusion that it
The purpose of this experiment was to identify the organism as gram-positive or gram-negative. The first step in the gram staining procedure was to heat fix the smear to the slide to ensure that the organism did not rinse off during the procedure. The second step was to apply crystal violet to the slide for twenty seconds. During this step both gram-negative and gram-positive organisms stained purple in color. After this step