Colored Light Absorption Lab Report

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Grishma Patel
Chloroplast Pigments and Colored Light Absorption
The purpose of executing this lab was to see how different wavelengths and colors of light are absorbed by chloroplast pigments. The goal was to see the variations of light dependant reactions of photosynthesis based on different types of light. Spinach was used by grinding it with acetone and acquire the thylakoids used in the experiment. DCIP was used in this experiment as the oxidizing agent that will turn blue to clear when in contact with light. The experiment required us to test first the relationship between wavelength of light and absorbance. The chloroplast pigments from the tissue were used to see which wavelength of light absorbed the most light. The result of this part of the experiment showed that the wavelength 432nm had the highest absorbance of 0.286. Next, we tested the absorbance of different colored lights. This part of the experiment tested the results of absorbance of red, green, white, purple, blue and no light using DCIP. Blue light showed the highest absorbance. Finally we found the chromatography pigments of different bands based on the separation of photosynthetic pigments.

The materials used in the
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The test tube was closed and shaken with a stopper which was periodically removed to alleviate pressure. The test tube was then left on the test tube rack to allow the solvents to separate. It had separated with the ether phase on top and acetone phase on the bottom. To see what light was being reflected, the tube was taken to the projector and it was recorded that the light reflected was red. The ether phase was used to paint with a brush approx. one inch above the bottom of a six inch chromatography paper. This step was repeated time

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