The solution homogeneity expelled, by centrifugation for 10 min. The sample was centrifuged and separated into two layers, and took the top of the sample is injected for HPLC (11,12). Measured concentration in total lipid: The total fats balanced concentration of the pesticide getting by dividing the measured pesticide residue concentration in the overalll tissue sample by the decimal fraction of the sample that consisted of ether-extractable lipid. The total lipid content of each specimen was estimated from its total cholesterol & triglycerides levels by using a summation method. Analytical results for organochlorine pesticides were reported on a lipid-adjusted basis (nanograms per gram or parts per billion) (14).
Conclusion The proposed HPLC method provides simple, accurate and reproducible quantitative analysis for simultaneous determination of atenolol and pregabalin in dosage forms, spiked and human urine. The cumulative excretion patterns of atenolol and pregabalin have a valuable clinical application by detecting the concentration of the two drugs in urine at any time during 24 hours after oral
This is what prevents the spots from becoming darker during the 15 minute reflux process. The initial amount was 2.003 grams and the end product weighed 1.468 grams. The results show that the crude sample that was made from the lab had around the same purity with that of the known sample, thus, the experiment was
During surgery, the infusion rates were adjusted at 10-15 ml/kg/h to maintain values of systolic arterial blood pressure and heart rate within ± 20% of baseline values. After induction of anesthesia al6-French gauge multi-orifice nasogastric tube was inserted and its correct position was confirmed by epigastric auscultation of injected air. Gastric content measurement (volume and pH) was made: 5min after induction of
The UV sensitive bands were purified using repetitive preparative TLC followed by crystallization. The identity of Ecdysterone was established by the following procedure: HPLC, with a Shimadzu LC-20, a Phenomenex C-18 reverse-phase Luna C18 which was used with a mobile phase of MeOH:Water (1:1) at 1.80 mL/min and the absorbance was monitored at 254 nm. Studies confirming the presence of a single peak of the isolated Ecdysterone, with a characteristic UV absorption at 246 nm were done using commercial standard Ecdysterone (Sigma) (Figure 2 A and B).
The analysis was carried on C18 shim- pack GIST (150mmx 4.6mm 5µ) column used as stationary phase. A freshly prepared mobile phase consisting of methanol: potassium dihydrogen phosphate buffer in ratio of (30:70 v/v), PH-3 adjusted using ortho phosphoric acid (OPA) these were filtered by 0.45µM Whatmann filter paper and sonicated before use. The flow rate of mobile phase was 1ml/min. The detection was carried out at 220 nm and run time was around 10 minutes. Selection of wavelength A UV spectrum of drotaverine hydrochloride, ethamsylate, tranexamic acid in water was noted by scanning the solution in the range of 200-400nm.
Then five millilitres of sample “A” were placed in the test tube labeled “A”. This was then repeated with the next three samples. Each sample was visually observed and the colour of each was recorded. Next 20 drops of Benedict’s solution were added to each test tube and the test tubes were lowered into a hot bath at a temperature of approximately 80 degrees Celsius. All colour changes were recorded.
The effect of variations in flow rate of carrier gas and Vail temperature was studied. Under all the variations, system suitability requirement is found to be within the acceptance criteria and hence the proposed method is robust. The relative standard deviation of area counts for Methyl Bromide peak obtained from six replicate injections of standard solution should be not more than 15.0%.The data of Robustness is following table. Methyl Bromide Robustness (Flow variation) System Suitability Parameter 2.59mL/min (Flow Minus) 3mL/min (Control) 3.5mL/min (Flow Plus) % RSD 6.5 5.11 7.21 Methyl Bromide Robustness (Vail Temperature variation) System Suitability Parameter 35°C (Temperature Minus) 40°C (Control) 45°C (Temperature Plus) % RSD 7.37 7.07
In the round-bottom flask (100 mL), we placed p-aminobenzoic acid (1.2 g) and ethanol (12 mL). We swirled the mixture until the solid dissolved completely. We used Pasteur pipet to add concentrated sulfuric acid (1.0 mL) to the flask. We added boiling stone and assembled the reflux. Then, we did reflux for 75 minutes.
Filtered the solution through 0.45 µm nylon filter Buffer having pH 3.70 used as Mobile phase A and mixture of methanol, acetonitrile and tetrahydrofuran (50: 50: 2 v/v/v) were used as Mobile phase B. Diluent: Prepared a mixture of buffer and methanol (80: 20 v/v). Placebo preparation (Placebo I): Weighed accurately and transferred 255 mg of placebo to a 100 mL volumetric flask. Add 40 mL of methanol and sonicated for 10 mins and add about 30 mL of diluent and again sonicate for 15 mins. Allow to equilibrate at room temperature (RT) and dilute to volume with diluent. Filter the solution through 0.45 µm nylon filter (25 mm) by discarding first few mL of the filtrate.