Moreover, it is less dense than water and is slightly soluble in water. Benzyl Acetate Benzyl acetate is a benzyl ester of acetic acid It has a boiling point of 212°C and a melting point of -51°C. Similarly, Benzyl acetate is also a colourless liquid and has a floral scent resembling Jasmin. It is soluble in a variety of things which include: ethyl alcohols, fixed oils, kerosene, paraffin oil, e.t.c and it is insoluble in glycerin. Muscone Moscone is also known as “3-methylcyclopentadecanone”, has a boing point of 320°C and a melting point of 8.6°C.
Shake c) Slowly remove the lower aqueous layer through the stopcock into the same 500 ml beaker in which the solution is there. d) Pour the upper solvent layer through the neck of the funnel into a clean 125 ml Erlenmeyer flask. Return the aqueous solution from the 500 ml beaker to the separatory funnel. Add another fresh 40 ml of solvent to the funnel and again extract the aqueous solution as you did in b)
The chemical or the bio-chemical parameters that affect the treatment process or the applications are 5 day BOD, COD, Total Nitrogen content, Free Ammonia, Total Phosphorous, pH, Alkalinity, Total and Fecal Coliforms (Alsulaili and Hamoda 2015, Edwin et
MDA level was determined by thiobarbituric acid reactive substances (TBARS) in serum, based on the reaction between MDA and TBARS. Standard Malondialdehyde solution in 5 mL of volume was processed along with test samples. 1.5 mL of 0.8% of TBA was added to 1 mL of the serum sample. Then 0.4 mL of 8.1% sodium dodecyl sulphate and 1.5 mL of acetic acid was added. The mixture was finally made upto 5 mL with distilled water and placed in hot water bath at 95ºC for 1 h. After cooling, 1 mL of distilled water and 5 mL of the mixture of n-butanol and pyridine (15:1, v/v) was added.
Estimation of total Flavonoid content by AlCl3 reagent method The total flavonoid content of methanol extract of leaves of A.malabarica was determined by the AlCl3 reagent method . The extract (500 µg/mL) was mixed with 0.5 mL of 5% NaNO2 solution and allowed to stand for 5 mins. Then 0.3 mL of 10% AlCl3 solution was added and the mixture was allowed to stand for further 5 min. Finally, 1 mL of 1 M NaOH solution was added, and the final volume of the mixture was brought to 5 mL with distilled water. The mixture was incubated for 15 mins at room temperature and absorbance was measured at 510 nm.
Sample Preparations Unknown water and reagent blanks are prepared in the same manner. 20 ml test tubes are taken and 5ml of water is pipetted into each of the test tubes. The pesticide standards are now inserted into the test tubes at concentrations of 0.1,0.2,1,2,5,10 and 30 ng/ml-1. The mixtures are mixed and kept in equilibrium for 30 minutes. After the 30 minutes is done, the tests tubes are then immersed in a 100 degrees Celsius methanol water bath for 15 minutes.
First, 50 mL of the sample was placed into a 250 mL Erlenmeyer flask, and onto a stirring plate. Then, the pH of the solution was measured and adjusted to be within the range of 4 and 6, using nitric acid and sodium hydroxide. After the pH was optimal for the experiment, a single mL of indicator- acidifier reagent was added to the sample. Then, 50 mL of mercuric nitrate was place into a burette and titrated with the sample until the color of the solution turned from blue to purple. The volume of titrant used for the reaction to reach endpoint was recorded.
After 20 minutes, the tubes from the bath was carefully removed, cooled in a beaker of cold water, then 8.0mL of 50% ethanol was added and mixed well. The absorbance of each tube was determined at 570nm and the absorbance were plotted against amount of standard glycine. For determination of tyrosine content using Folin & Ciocalteu’s reagent, seven tubes were set up. Then, 1.6mL of 0.5M Na2CO3 was added to each of the tubes and were mixed thoroughly. 0.4mL of the Folin & Ciocalteu’s reagent then were pipette in, mixed thoroughly, and allowed to stand for 10 minutes.
4.2 Physical Analysis 4.2.1 Density Test Table 4.1 shows, the samples of undoped calcium phosphate pellets using various sintering temperature. From the experiment, different sintering temperatures are used such as 1000ºC, 1100ºC and 1200ºC. Each sintering temperature have five samples are being used to measure the density of the samples. After analysis, the average density will be collect and recorded. The summary that can get from the table is the mean density for 1000ºC, 1100ºC and 1200ºC sintering temperature is 3.24, 3.26 and 3.25 each respectively.