Chrozophora Tinctoria Method

MATERIALS AND METHODS

Fresh leaves of Chrozophora tinctoria (Ch.t) plant were collected, in July 2007, from Derelbalah area, Gaza strip, Palestine. The leaves were separated and dried under shade for about a week. Then leaves were crushed to moderately coarse powder and kept at a dark and dry place. Two hundred grams of the fine powder were boiled in about 1 Liter of distilled water (100°C) up to 20 min. The obtained dark brown extract was cooled at room temp. The cold decoction was filtered with a cheese cloth to obtain clear solution. Then the solution was evaporated to dryness in a water bath at 55°C. The deep brown solid residue was stored in a refrigerator at 4°C until further use[ ]. When needed; the residue was suspended in

On the other hand, Animals from both experimental and control groups were decapitated at weekly intervals starting from the first week. Blood was then collected into 10 ml centrifuge tubes without any anticoagulant. The centrifuge tubes were left for about 15 min. to allow blood coagulation. Clear serum samples were separated by centrifugation at 3000 r.p.m. for 20 min. and then transferred into Eppendorf tubes and stored in a deep freeze (-20oC) for biochemical analysis. However determination of glucose was carried out on fresh serum samples.

Determination of Biochemical parameter

Serum glucose, total protein, albumin, globulin, urea, creatinine and uric acid were analyzed by Kone lab 60 Auto analyzer in Al Shifa Hospital Clinical Chemistry Laboratory.

Serum Glucose was determined by glucose oxidase (GOD)[ ] using LabKit kit, Spain. Serum total protein was determined by Biuret method using DiaSys kit spain[ ]. Serum Albumin was determined by Bromocresol green using Bio Systems kit Spain[ ]. The concentration of globulins were calculated by the following equation: Concentration of globulins(gm/dl)= Total protein –Albumin. serum urea was determined by urease - glutamate dehydrogenase (GDH) using Bio Systems kit, Spain[ ]. Serum Creatinine was determined by Alkaline Picrate method using BioSystems kit, Spain[ ]. Serum Uric Acid was determined by Uricase