There group of rats were used in our study (n= 6–8 rats/group) as follows Control-group received vehicle (corn oil daily) for 2 weeks, VPA-group received VPA alone (500 mg/kg PO, daily) for two weeks and VPA+DHA-group received VPA (500 mg/kg PO, daily), followed by DHA (250 mg/kg PO, daily) for two weeks. After two weeks of treatment, rats were terminated using sodium pentobarbital (50 mg/kg, IP) for liver collection. Liver was isolated, weighed, aliguoted in few tubes and snap frozen in liquid nitrogen. A 10 % (w/v) liver homogenate was made in phosphate-buffered saline (PBS) (pH 7.4) for the assay of hepatic TBARs, NADPH, NrF2, and HO-1. Other frozen liver samples were homogenized in RIPA buffer for western blotting (n=4/group).
Therefore, this work was designed to evaluate the analgesic, antipyretic as well as the anti-inflammatory effects of EVOO and compare it with ibuprofen individually and in combination with two different doses of IBU (therapeutic dose 100mg/kg and low dose 40mg/kg), on different animal models in albino mice. MATERIAL AND METHODS Animals: A total of 132 adult healthy male Swiss albino mice, weighting 25-30 g were bred in the animal house of Kasr Alainy hospital. The mice were left for 2 weeks for acclimatization before use. Animals were maintained at a constant temperature (24+1C) with 55% humidity, on a 12 hrs light/dark cycle. The night before the experiments, food was withdrawn from the cages but water was given ad libitum.
The platform was placed near the centre of a quadrant and rats were released into the water from 1 of the 3 remaining quadrants to search for the platform. The time taken to find the hidden platform [escape latency (EL)] was recorded in each trial. Even after 90 seconds if animal did not reach the platform, it was guided to the platform and allowed to remain on platform for
2-aminoanthracene (2AA) is a PAH that has been associated with the onset of early diabetic symptoms. This study was conducted to identify if 2AA dietary ingestion would induce T1D renal injuries. To accomplish the research objective, three weeks old male Sprague Dawley rats were assigned into three 2AA dietary ingestion groups (0 mg/kg, 50 mg/kg and 100 mg/kg) for twelve weeks.
The specimen that were utilized were Sprague Dawley adult male rats (10 rats). They were euthanized by 0.5ml of sodium pentobarbital, an intravascular injection, and then weighed. Afterwards, these animals were assigned randomly to a horizontal or vertical position (5 rats per group). The rats in the horizontal group were placed on a supine, head-up, position while the vertical group were placed on a prone, head-down, position. The room temperature was kept at 22°C.
For investigation of treatment effect 21 days after the alfalfa solution administration, blood sample of all animals prepared and Serum concentrations of total cholesterol (Chol), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), triglycerides (TG), Very-low-density lipoprotein cholesterol (VLDL), glucose, the liver enzymes aspartate transaminase(AST) and alanine transaminase (ALT) levels were measured at the end of supplementation period in all studied groups. Histological studies After a 3-week experimental period and the last blood sampling, the whole pancreas and liver was removed after sacrificing the animal and was fixed in 10% formalin for histopathological examination. Sections were cut and stained by hematoxylin and eosin (H&E) for histological examination(10). Statistical Analysis: Statistical analyses were conducted using SPSS software version 13.0 (SPSS Inc., Chicago, Illinois, USA). Between-group comparisons of biochemical factors were carried out using Kruskal-Wallis test.
The above collage of pictures shows affect of scavenging on decomposition. The pictures on left side were from scavenged piglet and the pictures of right side were from controlled piglet. The pictures opposite from each other are taken at same day and same time but the decomposition in scavenged piglet occurred quickly as compared to controlled piglet. The bottom picture of right side shows rupture of skull of the controlled piglet. Which shows that, the scavenger wants to sneak that piglet from the cage The figure 1 illustrates the daily maximum temperature over the 13 days period of experiment.
The characteristic feature with DOXh therapy is the appearance of red color of urine within the first hours to days after administration. Studies carried out by (Yesair et al., 1972), 10-20% of the drug is excreted from feces within 24 hrs while next 40-50% is excreted within 7 days. For pegylated liposomal DOXh (Doxil® or Caelyx®), most of the drug is cleared with an elimination half-life of 20–30 hours (Gabizon et al.,
.4 Agar Disk Diffusion The antimicrobial tests were carried out according to disc diffusion tests (Lennette et al., 1985, Kim et al., 1995). Cells were streaked on MHA agar plate to obtain colonies at 37°C for 16 hrs after which they were resuspended in sterile saline solution to give an O.D of 0.1 at 600nm. On solidified 2% MHA plate, the bacterial culture is swabbed and sterile disc impression was made, 10 µl plant extracts was loaded on the agar plate. After 10 minutes the plate is incubated at 370C The diameter of the clear zone was measured. Fig 9: Agar disk diffusion 5.5 Time Kill Assay The basic concept of the Time-Kill Kinetic study is establishment of the rate at which a microorganism is killed by a product as a function of survival
Disposable lancets are used and new lancet is used for every patient. All equipment are sterilised using UV Steriliser for 5 minutes before the cupping procedure. 8.3 When is the best and beneficial time to do cupping? The best and beneficial time to do cupping is 17th, 19th and 21st of the lunar month. Cupping should also be done when one is on empty stomach for at least three hours.
Experimental procedure (real life) Complete the ‘experimental setup’ for the guinea pig ilium experiment as shown in Fig 1. The agonist, acetylcholine is added to the organ bath at a concentration of 1 x 10⁻⁸ M where it will follow the 3 minute cycle. After 30 seconds the results are recorded. After results are recorded, washing of the organ bath and ilium is conducted (note. Called wash out).
A patient presents to an outpatient center to undergo a colonoscopy for possible polyp removal. By hot biopsy forceps, a polyp is removed from the descending colon, and by snare technique, a polyp is removed further up into the transverse colon. What CPT code(s) should be reported by the FACILITY? a.) 45385, 45384-59 b.)
In the in vivo microdialysis experiment, the laboratory rates were put asleep using Equithesin to allow the experimenter to place concentric microdialysis probes in the left hemisphere of the brain. The probes were either placed in the shell of the NAc or in the core of the NAc. Twenty-four hours after the probe was implanted the experiment was performed on free moving rats. A solution that was prepare with 147 Ringer’s, 4 KCl and 2.2 CaCl, was administered into the probe at a constant rate. Samples from the rates were collected and divided.
Place C Elegans into the plates with the E Coli and leave for 24 hours. Examine the C Elegans to insure that the C Elegans have survived at the room temperature and continue to have multiple C Elegans surviving. Once this is done prepare the dilutions of all the subjects which we are testing. Start with 1% solution for Nitrate-N 100ml and move 10ml of the first well into the next. Fill the well with 90ml dh20 to reach 100ml.