Kreb Cycle Lab Report

CITRIC ACID CYCLE / KREB CYCLE:

DEFINITION:

Regarding the reaction of living body, which provides energy for acetic acid or acetyl equivalent ozone-based phosphate bonds (such as ATP) for storage - it is also called the citric acid cycle, tricarboxylic acid cycle.

PRINCIPLE:

The citric acid cycle also known as the tricarboxylic acid cycle (TCA cycle), the Krebs cycle, or it is a series of enzyme catalyzed chemical reactions, which has central importance in all living cells that use oxygen. In eukaryotic cells, matrix of the mitochondria contain the citric acid cycle present in then. In aerobic organisms,a metabolic pathway involved the conversion of carbohydrates, fats and proteins into carbon dioxide and water so that it generate a form of

The first reaction is catalyzed by citrate synthase enzyme. Once the oxaloacetate and acetyl CoA are joined, the water molecule attack at acetyl group of acetyl coenzyme A and release the coenzyme A from complex of oxaloacetate and acetyl CoA to formed citrate. Citrate synthase:(enzyme)

The enzyme citrate synthase is present in nearly all living cells and act as a pace-making enzyme in the citric acid cycle’s first step. Citrate synthase is present in eukaryotes but is is made up of nuclear DNA instead of mitochondrial. Oxaloacetate is regenerated after the completion of one kreb cycle.

REACTION 2: Formation of Isocitrate:

The next reaction of the Kreb cycle is catalysed by acontinase enzyme. In this reaction overall two H2O molecules are generated one water molecule is removed and other water molecule is put added into another location. The overall effect of this reaction is that the shuffling of -OH group from position 3 to 4. The yield that get is isocitrate