3.1 The isolation of Aeromonas from several pond waters, healthy fish, and infected fish The isolate of A. hydrophila grown for 24 hours at 37C on Rimler-Shoots+novobiocin medium should show bright yellow color with white edge. Figure 1 shows the control of isolate A. hydrophila ATCC 7699 grown on RS+novobiocin medium. Isolate selection on RS medium resulted in 95 isolates, presumed to be A. hydrophila, which would run the phenospecies test (morphology and biochemistry), based on the protocol of SNI 7303 (2009), plus one control isolate the A. hydrophila ATCC 7699 obtained from Microbiologic Co. Figure 1. The Isolate of A. hydrophila ATCC 7699 Grown on the Rimler-Shoots Agar Medium + Antibiotic Novobiocin at 37C for 24 Hours 3.2 The
Remove excess water from the surface of the gram stained glass slide and observed under 1000X oil immersion microscope. Gram-staining have performed for Staphylococcus aureus (control); Enterococcus faecalis (control); Nostril microflora in NA, MSA, and PYCa. Gram-staining provides results of the bacteria morphology, type of gram-stain. Catalase test was also done prior to gram-staining. MicrobactTM Biochemical Identification Kit was used for identifying gram-negative aerobic and facultatively anaerobic bacteria.
1) Present the gel electrophoresis image of plasmid digestion experiment with the correct label. Describe the results of the experiment and list down possible problem that you observe based on the electrophoresis image. Plasmid P1-1 and P1-2 undergo single digestion. Plasmid P1-3 undergoes double digestion. Single digestion is only one restriction enzyme which has been used to digest a DNA.
To determine these percentages however, an indirect measurement was performed where RuBisCO is converted on a total protein basis assuming the chlorophyll: total protein mass ratio is 0.0421. Therefore, Losh et al. conducted experiments where RuBisCO was measured with Quantitative Western blots using an antibody which binds to a conserved region of the large subunit of RuBisCO. They concluded that RuBisCO represented < 6% of total protein in eight species of microalgae. Furthermore, they concluded that unlike in plants, RuBisCO does not account for a major fraction of cellular nitrogen in
Movincool Classic Plus 26, USA). Pupae collection and Irradiation: Eggs were collected and washed with distilled water and sieved with a very fine screen and then measured volumetrically. Larvae of oriental fruit fly were reared in the laboratory using artificial standard larval diet and kept in larger bowls contained 1.5 to 4 cm thick sawdust used for pupation. Sawdust was sieved and collected pupae were transferred into Petri dishes and irradiated by exposing them to gamma radiation from a radioactive Cobalt-60 source. To optimize the radiation dose of sterilization several batches of 5 and 6-day-old pupae were irradiated at 30, 40, 50 and 60 Gy
The samples were filtered by vacuum over pre-weighed and wetted glass fiber filters (24 mm GF/C; Whatman; Kent, UK). The samples on the filters were rinsed with 5 ml stop solution to remove non-specific, cell surface binding of 3H-FLC. The filters with fungal balls were either allowed to dry for 24-48 hr or were baked in a drying oven for 15 minutes at 95° C. Each dried filter containing fungal balls was then re-weighed to obtain the dry mass of each fungal sample. The filters were finally transferred to 5 mL scintillation vials containing 3 ml of scintillation cocktail (Ecoscint XR, National Diagnostics, Atlanta GA). Radioactivity associated with the fungal sample on each filter was measured in a liquid scintillation analyzer (Beckman Coulter, LS 6500 multipurpose scintillation
Amir Ahemedin Ms.Buckley Genetics 11/06/15 Transformation of E.coli Lab Purpose The purpose of this lab is to genetically engineer the E.coli strain by introducing two genes, the green fluorescent protein gene (GFP) and the ampicillin resistant gene (AMP). Then observe whether or not the E.Coli strain would take up these genes and become fluorescent. Background Information In this lab, bacterial transformation was one of the three processes that occurred when genetic material is introduced to a bacterial cell. Bacterial transformation is important because it allows for the cloning and movement of DNA between strains. This transformation usually occurs within plasmids, which are closed circular molecules made up of double stranded DNA.
However in this essay we will focus more on the application of biofuels through the conversion of sugar to alcohol, otherwise known as fermentation. The understanding of fermentation first came into light in 1789 by a french chemist known as Antoine Lavosier, who studied the transformation of substances. Through quantitive chemistry, he studied the mechanism of fermentation by estimating the general proportions of sugar and water molecules in sugarcanes with the with the end products such as carbon dioxide and ethanol; he also added yeast. In his conclusion, two thirds of the sugar was reduced into ethanol and the other one third was
It has ability to produce melanin pigment by using various precursors of melanin in the media. I] Culture Media For primary isolation: - . For primary isolation of cryptococcus bacteriological media like blood agar, chocolate agar and Brain heart infusion agar, Cystein-heart haemoglobin agar, bird seed agar and sunflower seed agar can be used (Chander J, 2009). On Blood and brain Heart Infusion Agar-Cryptococcus grows at 370 C as buff coloured mucoid colonies. (REFERENCE) Chocolate Agar- Used for capsule demonstration.
We took 2 Petri plates containing L-agar and spread 50µl from dilutions 〖10〗^(-3) and 〖10〗^(-5) respectively on L-agar plates by using spreader and incubated them at 37°C for 24 hours. After 24 hours incubation, we selected isolated colony and streak it on L-agar containingPetri plates to purify it. After 24 hours incubation, we got our purified colony, now we checked either it is nitrogen fixing bacteria or not, for this purpose we performed “Nitrite Detection Test”. Nitrite Detection Test: We made mother culture of our strain. We took 3 test tubes and added 3 ml ammonium sulphate broth and autoclaved them.