Chitosan Lab Report

Celecoxib loaded chitosan microspheres prepared by emulsion cross-linked method were brownish-yellow colored free flowing particles. The desirable characteristic of the processing variable parameters, i.e. polymer concentration, emulsifier concentration and rotational speed to formulate different batches of celecoxib loaded microspheres. The effect of the various variables on these characteristics is described below:

Particle size

The average particle size was found to be dependent mainly on the concentration of the drug-polymer ratio which was varied from 1:2 to 1:8; and the mean diameter of microspheres was in the range between 8.42 to 10.32µm. An increase in the particle size of microspheres with increase in the concentration of chitosan was clearly observed. This leads to the formation of bigger droplets of the internal phase in the emulsification step in the preparation of microspheres.

The particle size decreased from 13.63 to 6.52 µm with increase in the rotational speed from 1000 to 2500 rpm. This is due to the fact that higher stirring speed provides the required energy to the chitosan solution to be dispersed as fine droplets in the external oily phase and therefore microspheres with smaller particle size and narrower size distributions are formed. The study results are in concordance with previous published reports [26].

Span 80

FTIR spectrum of the celecoxib showed a characteristic S=0 symmetric and asymmetric stretching’s at 1159 and 1346 cm-1 respectively. Medium intensity peaks at 3333 and 3227 cm-1 were seen as doublets, which are attributed to the N–H stretching vibration of sulfonamide. The spectrum of Eudragit S-100 coated microspheres of celecoxib containing chitosan showed peaks at 3333 and 3227 cm-1 due to celecoxib, at 1738 cm-1 with respect to Eudragit S100 and at 1346 and 1159 cm-1 due to celecoxib and Eudragit