Clostridial Toxins: A Case Study
Rapid tests for detection of main Clostridial toxins
Pérez-Etcheverry Diana* and Lorenzo-Ferreiro Carmen
Laboratorio de Biotecnología del Instituto Polo Tecnológico de Pando - Facultad de Química, Universidad de la República. Canelones, Uruguay. *perezetcheverrydiana@gmail.com
Abstract: The genus Clostridium is ubiquitous[1], because of this they find easily their way into wounds, foods and feeds, being the cause of serious illness on human and domestic animals. Manifestations and pathology can range from mild food poisoning to death [2-13]. Outstanding to their high toxicity, and the rapid evolution of infection with serious consequences [14-19], it is important to detect it rapidly. One approach is to have rapid detection tests to major
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Gynecological infections in human after childbirth and abortion[147-150], post-operative infections[4], and necrotizing soft tissue infections associated with heroin use[4, 151] can be attributable to infection with C. sordellii. Two toxins are a main responsible virulence factors of C. sordellii: hemorrhagic toxin (TcsH) and lethal toxin (TcsL)[152-155].
The mortality rate for patients with C. sordellii infection is 40-69%, and most patients die within days or even hours after the initial presentation[4]. For that reason, premature diagnosis of C. sordellii infection is of main importance; the low prevalence of the illness and the fact that the initial symptoms are nonspecific make a precise diagnosis very difficult. The time lapse between the beginning of symptoms and death is frequently so short that does not allow to initiate an empirical antimicrobial therapy.
Currently, the C. sordellii diagnostic is determined by means of anaerobic cultures of blood, which is not only time consuming but also is limited to certain laboratory facilities that not all hospital laboratories have, thus do not perform antimicrobial testing on
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