Coagulase Test
Coagulase test Coagulase is an enzyme that causes plasma to clot by activating prothrombin to form thrombin which then catalyzes the activation of fibrinogen to form fibrin. Coagulase test was performed one drop of citrated human plasma was added on a slide by using a plastic loop or wooden stick,. Mix well and clumping was observed within 10-15 seconds indicate the coagulase positive test. Citrate test- Fresh (16- to 18-hour) pure culture was used as inoculation source. A single isolated colony was slightly streak on the surface of the simmon citrate agar slant. Incubate at 35ºC for 18 to 48 hours. Growth was observed at the slant surface and the color of medium was changed from intense green to a deep Prussian blue. Determination …show more content…
The silver colloidal particles obtain a negative charge because of to the adsorbed citrate ions; The absorption peak was observed at about 420nm which is the typical characteristic absorption peak for Ag nanoparticles. UV absorption peak of chitosan-Ag nanoparticles was observed by other researchers in the range 410–450 …show more content…
The 1 µm size of chitosan conjugated silver nanoparticles confirmed their nano size structure.The profile of FTIR spectrum the main absorption of ch-agnps as 3345, 2123.71, 1639.43, 1045.09cm-1. The FTIR spectrum of the ch-agnps show OH stretch at 3345.78 and N-H bending at 1639 cm-1. In the FTIR spectrum of ch-agnps, the shift of chitosan peak is observed which shows amplification in the intensity of c-o stretchIn the present study all the clinical isolates were identified by observed their colony Morphology,s and biochemical characterization. as e.coli, p.aeruginosa, s.typhii and s. aureus. The results of this antibacterial activity of ch-ag np suggested that the presence of a small percentage of Ag nanoparticles in the composite was enough to enhance antibacterial activity significantly. Chitosan conjugated silver nanoparticles exhibit strong bacteriocidal activity at different concentration as compared to gentamycin. According to the result we can use chitosan conjugated silver nanoparticles to treat a various bacterial infection caused by pathogenic bacteria. Ch-AgNps can also help to reduce the problem of toxicity and to avoid the problem of multi drug resistance The broad spectrum of bioactivity of AgNPs makes them promising agents not only to fight. Both chitosan and silver nanoparticles are antibacterial agents
Ronald Bates Case Study
1748 Words | 7 PagesIV bolus of unfractionated Heparin or Subcutaneous injection of low molecular weight heparin (LMWH) may be used to prevent the formation of new blood clots. Nursing consideration: Require regular monitoring of activated partial thromboplasitn time (aPTT) and needed frequent heparin dose changes (Brunner and Suddarth’s, et al, 2010: 765). Fibrinolytic therapy: This therapy is given to dissolve the thrombus in the artery and restore the blood flow. There are two fibrinolytic drugs which are streptokinase and Recombinant tissue plasminogen activators (r-TPA) which includes Alteplase, reteplase and tenecteplase (Brunner and Suddarth’s, et al, 2010: 772).
Pglo Transformation Lab Report
664 Words | 3 PagesOn the first negative control plate, there should have been no growth on the plate with agar and ampicillin and regular E. coli cells. On the negative control plate with just agar and E. coli cells there should have been growth but the colonies would not
Pglo Lab
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Lb-Pglo Lab Report
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Gonorrhoea Case Study
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Essay On Haemostasis
1705 Words | 7 PagesDegradation of fibrin is termed fibrinolysis. The fibrinolytic pathway is a complex physiological pathway controlled by action of a series of cofactors, inhibitors, receptors. Dysregulation of this pathway is associated with different pathologies (e.g. coagulopathies, disseminated intravascular coagulation (DIC) or congenital bleeding disorders). Degradation of fibrin is performed by serine protease plasmin, which is present in blood as a proenzyme, plasminogen, and needs to be activated by tissue plasminogen activator (tPA) and urokinase. Disturbance in haemostasis with abnormal bleeding may be caused by thromocytopenia, platelet function disorder, or defects in blood coagulation.
Clotting Cascade
765 Words | 4 PagesIllustrate the clotting cascade and compare the mechanisms of action of the anticoagulants and their clinical indications. The Clotting Cascade occurs when several circulating proteins interact in a cascading series of limited proteolytic actions and is the activated when intravascular conditions cause thrombi to develop. “At each step, a precursor protein is converted to an active protease that activates the next clotting factor, and finally, a solid clot is formed”(Woo & Wynne, 2011, p. 445). The process of clot formation and dissolution of the clot following repair of the injured tissue is termed hemostasis and is comprised of four major events: 1. Initial Phase: Vascular constriction in which the flow of blood to the area of injury is limited and Factor VII is activated.
Theories Of Threart
945 Words | 4 PagesTHROMBOS COMPOSITION Analyses were performed on 44 of the 45 thrombi collected. One thrombus was not analyzed because the SEM preparation had failed. Fibrin fibers were the major thrombus component, representing more than 60% of their composition.
Summary: Pathophysiology Of Hematuria
490 Words | 2 PagesPathophysiology of Hematuria Throughout the process of filtration by the kidneys, along with the help of the ureters, the bladder and urethral to expel urine from the body, there are many possibilities that abnormalities can develop that could compromise the healthy characteristics of the filtration process. Hematuria, for example, is the irregular presence of red blood cells in the urine due to dissected variations of trauma in the urinary tract. There are two types of causes of hematuria, the glomerular and nonglomerular cause. The glomerular cause is trauma within the nephrons of the kidneys, where red blood cells enter the urine stream from the glomerulus.
Kirby Bauer Diffusion Test Lab Report
624 Words | 3 PagesThe sterile cotton swab was inserted in the S. epidermidis culture and twirled around to obtain a specimen. The entire plate was inoculate with the swab from top to bottom, to achieve a lawn of growth. The dry forceps was used to remove the antibiotic disk into the appropriate spot on the plate. This process was repeated for the all antibiotics with aseptic technique being used. The plate was incubate with lid up on the bookshelf at room temperature for 48 hours.
Kinetic Report
759 Words | 4 PagesINTRODUCTION Gentamicin, which is a broad spectrum aminoglycoside antibiotic (Fig. 1), belongs to the class of medicinal compounds capable of inhibiting the growth of Gram-positive and Gram-negative bacteria [1]. Gentamicin is one of the most effective drugs used in the treatment of serious suppurative and septic processes, especially those that are caused by Gram-negative microorganisms. The advantages of gentamicin over other aminoglycosides (kanamycin, neomycin) are its activity towards Pseudomonas aeruginosa and microorganisms of the Serratia–Klebsiella–Enterobacter group, a faster bactericidal effect, and the rare development of gentamicin resistant strains. Several methods used for determination of GT including colorimetric [2,3], amperometric enzyme-immunosensors [4], flow injection chemiluminescence [5], spectrofluorometry [6,7], capillary electrophoresis with UV detection [8], liquid chromatography (LC) [9–14] and high performance liquid chromatography (HPLC) [15–23]. Kinetic methods have many advantages over other spectrophotometric methods due to their high selectivity and elimination of
Chloramphenicol Ophthalmic Hydrogel Lab Report
735 Words | 3 PagesWith the method of scratch, the results of the saucer MIC subculture incubated at 37 °C for 18 h. The results form bacterial colonies scratches subculture. If there were scratches (+) then showed the presence of growth, when no scratches (-) then the growth does not occur. The data obtained was made in the form of a
Tacrolimus Experiment
4228 Words | 17 PagesIn each experiment the 250 ml flask contained 50 ml production medium and three factors namely ammonium sulphate, yeast extract and propylene glycol, were varied in their levels according to the design of the software. All other factors were kept constant (glucose 1 %, glycine 0.2 %, temperature 28 °C, pH 7.0, agitation rate 200 rpm). All the flasks were incubated at 28 °C in Kuhner incubator shaker. Samples were taken at 9
Bacterial Species
1566 Words | 7 PagesThe agar will remain a green color such as in E.coli, which is the negative control for the citrate test. No growth has occurred. When unknown 4 was tested, it presented a strong blue color, resembling that of the positive control E.
History Of Nano Robotics
1705 Words | 7 PagesNano scale materials are proving attractive to a new generation of devices due to their unique properties. They are used to create fast responding sensors with good sensitivity and selectivity for the detection of chemical species and biological agents. Nanosensors for Chemical and Biological Applications provides an overview of developments brought about by the application of nanotechnology for both chemical and biological sensor development. Artificial intelligence(AI) is the intelligence which is exhibited by the machines. AI research is highly specialized, in the field of the communication.
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