2) Usage of water in step (5):So that after Estrification is completed , any excess unreacted acetic anhydride is hydrolyzed. 3) The addition of half-saturated NaCl (in step 6): to help in separating the two layers. 4) The usage of a Base (step 7): to neutralize remaning acid . 5) Usage of concentrated sodium chloride in step (9):To dry the ester from any dissolved
A separatory funnel is attached to the clamp stand with a conical flask below as a receiving flask for the unwanted solution. The separatory funnel works by adding a solution to the mixture and blocking the top with a bung. Then turning the funnel upside down and right way around combines the mixture and the solution aiding in separating the ester from the aqueous layer. The aqueous layer is then removed/released by turning the head of the funnel. The bung must be removed at this stage as Carbon Dioxide is released.
Add the colony in to the prepared 0.85% NaCl solution containing bottle. V. Mix the contents without clumps of bacteria floating to form a homogenous suspension. VI. Flame the tip of the bottle and close it with a lid. • Inoculation of the strips I. Inoculate the bacterial suspension using sterile pipettes into the cupules (wells) by holding the strip to an angle up from the table II.
Place 8 clean non-metal bowls on your working area and label them with the following labels:, dH20 rinse 1, 2 and 3, Tap water, Powerade and Orange juice. Pour each liquid into the appropriately labeled bowl. Place the conductance sensor in the tap water and set the multimeter to measure current in the 200mA range. Make sure the conductance sensor is completely submerged. Record the current and make sure the unit is milliamps.
We then take the sample out of the water and immediately wipe it thoroughly, after which it is placed in the calorimeter with tap water. We then stir the water around the sample in order to quickly attain thermal equilibrium. We record the final temperature once thermal equilibrium of water and the sample together
Making sure that the beaker is dry before hand. Get the stopwatch ready to record the time of the reaction. Apply one of the 100mL Acetic acid into the beaker and start the stopwatch as soon as the two substances meet. Record the qualitative data observed in the
Later, the hydrolyzed samples were allowed to cool. The sample was filtered using Whatman filter paper; No.42 into a round bottom flask and evaporated till the acid content is completely removed. Finally, the volume was made upto 5 ml with 0.05N HCL and derivatized to phenyl thiocarbomyl amino acid before injected to HPLC. Fatty acid