Cocos Nucifera Linn: Monocotyledonous Family

Cocos nucifera Linn., is a member of the monocotyledonous family Aracaceae (Palmaceae) and it is grown as a plantation crop in more than 90 countries. It is native to coastal areas of Malaysia, Indone-sia, Philippines and Melanesia (Chan & Elevitch, 2006). In the tropics, the coconut plant has the vari-ous number of uses. Plant parts such as endosperms were extracted for coconut milk or oil (Adkins et al., 2005) which were used as cooking oil, hair oil and lamp oil and as an essential ingredient in soap making (Chidambaram et al., 2013) The water from immature nuts is drunk as a beverage, husk fibers are made into ropes or other construction material, and the whole husks and shells are used for fuel as a source of energy, (Chan & Elevitch,

Var. MATAG. Samples of Cocos Nucifera Linn. Var. MATAG inflorescence were collected from Department of Agriculture Terengganu’s farm. The samples were stored in ice-box to maintain its freshness prior to culture.

B. Optimization of Sterilization Procedure
The rachillae bearing inflorescence were cut off from the spadices and then were washed under run-ning tap water for 15 to 20 minutes to remove dirt and debris. The male inflorescence was isolated from rachillae and subjected to three different method of sterilization (as described in Table 1).

C. Media Compositions and Growth Conditions
The basal media used for this experiment were Y3 media (Eeuwens, 1976). For anther culture, the basal media were supplemented with the various concentrations of 2,4-Dichlorophenoxyacetic acid (2,4-D) (0, 10, 20, 30 and 50 mg/L) plus activated charcoal in different concentration (10, 20, 30, and 50 g/L). For ovule culture, only activated charcoal (4 g/L) with or without citric acid (50 mg/L, 100 mg/L) were added in the basal media. All the media contained 90g/L sucrose were adjusted to pH 5.8 and then solidified with 4 g/L gelrite. The medium was autoclaved at 121°C for 20 min. Cultures were incubated in a dark culture room at 25±2°C for eight weeks. Visual observation of culture was made every