Protassium Iodine Solution Lab Report
CHEMICALS REQUIRED
• Iodine solution
• Potassium iodine
• Sodium hydroxide
• Fehling’s A & B solution
• Concentrated HNO3
• Benedict solution
• Ammonium molybdate Figure 1 – soft drinks of three different brands
Physical analysis
APPARATUS USED
Test tubes
PH paper
DETECTION OF PH
1-2 drops of the sample of cold drink of each brand was taken and was tested by pH paper. The change in the color of pH paper was noticed and was compared with the standard pH scale.
COLOR DETECTION
Color detection is done by manually. Through naked eyes.
ODOUR DETECTION
It is detected by smelling or the smell or here we can say the gas evolved after opening the cane or bottle.
Chemical analysis
APPARATUS USED
Test tubes
Water bath
Conical flask
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The incubator shaker can be used for growth of just about any kind of cell including bacterial cultures, tissue cultures, and yeast.
Procedure Microbial analysis was done by serial dilution and then inoculation of sample into YPD Media. Preparation of media
• Firstly media was prepared by weighing and mixing the ingredient.
• Media constituents are – casein 0.5 mg Yeast extract 0.25 mg Dextrose 0.1 mg Agar 1.5 mg
• Mix the constituent into distilled water to make it 100ml media.
• After that keep the mixture on hot plate until it becomes little transparent.
• Autoclave the media.
• Pour the media into petri plates inside laminar air flow.
• Allow it to dry at room temp.
Serial dilution and inoculation of sample
• Serial dilution was done up to 10^3 inside laminar air flow.
• Before one hour of your work, start the laminar air flow 's main switch or
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