Introduction in 1861 thomas graham observed that crystalline substances can easily pass through semipermeable membrane while particles of glue gelatin and gum can not pass , depending upon size of pariticles solution is divided into three types true solution: solution which is completely homogenous and have (0.1 – 1 nm) particle size suspension: solution of suspended particles in liquid which settle down at the bottom e.g sand in water and particle size is bigger than 1000 nm
Colloids: colloids consist of dispersed particles distributed throughout system also called colloidal solution or colloidal dispersion .in colloids particles neither completely dissolve like solution nor settle down like suspension but intermediate between true
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. Depending upon dispersed phase and dispersion medium, there are eight types of colloidal solutions:
Classification of colloids
Depending upon type of interaction betwwen dispersed media and dispersed phase there are two classes of colloids
Hydrophilic colloids :
These are water loving colloids easily spread throughout medium and are easiy to prepare .these colloids are stable because there are strong forces of interaction between colloidal particles and liquid
These are also called Reversible colloids because on evaporating the liquid, the residue left will passes into colloidal state on addition of liquid.
Examples: agar is a reversible hydrocolloid of seaweed extract; it can exist in a gel or liquid state and can alternate between states with either heating or cooling. proteins -nucleic acids -micelles of soaps also form hydrophilic colloids
hydrophobic colloids:
These colloids, also known as emulsion are water repellent and lack the tendency to mix with water so are difficult to prepare. As there are weak forces of intearaction between water and particles so these are unstable
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Coagulation
In hydrophobic colloids the dispersed particles can be separated from dispersion medium as precipitate this is called coagulation. Normally coagulation is done by adding electrolytes which result in neutralizing the colloids particles which then start accumulating as clusters
However Coagulation can be carried out by adding concentrated salt solution e.g NH4)2SO4, Na2SO4 in colloidal solution , by heating with mineral acids which cause denaturation and by using ultrafilters.
Applications of colloids
Colloids have various applications in medicine, industries,
1. Most of our medicines are colloidal in nature. Colloidal particles are finely divided and can easily absorbed by our body so colloids are used as therapeutic agents in different areas
2. Larger number of our foods are colloidal in nature. Milk is an emulsion of butterfat in water protected by a protein. Icecream , mayonnaise, cheese, cream and butter are also colloidal.
3. Water contain colloidal charged particles, we can purify water by adding electrolyte like alum which remove the collidal impurities and purify the
Identification of bacteria within Unknown Culture #21 In this experiment, an unknown culture of two different types of bacteria was assigned to each person, a number of tests were performed to isolate and identify these bacterial cells. Based on knowledge from the previous experiments completed in lab, a basic understanding of each type of bacteria was used to create a flow chart that would aid the process of identifying the unknown bacteria within the culture. A gram stain that is performed initially will narrow down the types of tests certain bacteria will and will not respond to. In addition to the gram stain, some of the tests that were used include, a catalase test, an Eosin methylene blue (EMB) agar test, a bile esculin test, and a 6.5% sodium chloride (NaCl) test.
Our hypothesis was partially correct, the property changing substances did have the weakest coherency with the lowest drop counts of 23(carbonated), and 14(soap), and pure water did have the strongest bond. What we also found was the the salt also dampened the liquid’s ability to hold onto a penny in large volumes, as all the different salts had a drop average of 24(28x2 & 16), five less than Tap water’s drop count (30). This led us to conclude that pure water has the strongest bond and that all foreign materials weaken the coherency of water. This evidence has led me to believe that similar substances are attracted and are more coherent towards themselves, in this case the water pieces get separated and generally less connected on a really small level due to the obstruction of foreign objects, this is why the different salts perform better than the huge air bubbles or the slippery properties of soap since the latter is more obstructive and the former dissolves with the water and blocks less. When studying a few other groups’ conclusions and data we did indeed find differences: some groups had differing data where a solution had better coherence than water itself which led to differing conclusions.
Filtering, evaporating, centrifuging, and decanting something will only physically change it. Chromatography is used to separate different parts of a solution so that it can be identified. It can work because different substances have different attractions to things. Distillation can separate substances, such as salt water, as long as it has different boiling points. It can even be used to purify salt water but it is not cost efficient or energy efficient so it is not suitable for everyday use.
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
Determining the Identity of an Unknown White Compound Maxwell Yurs Group Member: E.B. Floersch, Alexander Medina, and Masih Horri College Science and Engineering University of Minnesota, Minneapolis October 12, 2014 The experiments that were completed were used to determine the identity of an unknown white compound to allow for proper disposal. These experiments included flame tests, pH tests, and conductivity tests. After each was completed, it was found that the identity of the white compound was Sodium Nitrate.
When using a sanitizer, frequent water testing is needed to ensure complete water sanitization. When testing ionization you are monitoring the copper
The Gastrocnemius Muscle of Rana pipiens is an Appropriate Model for Skeletal Muscle Contractile Kinetics When Compared to Peer-Reviewed Models Georgia Institute of Technology BMED 3110: Quantitative Engineering Physiology Laboratory I Section B: Team Baboons 16 November 2014 ABSTRACT The dynamics of skeletal muscle kinetics can be quantified using various experimental methods involving stimulated muscle contractions.
Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides.
Abstract: The purpose of this experiment was to identify given Unknown White Compound by conducting various test and learning how to use lab techniques. Tests that are used during this experiment were a flame test, ion test, pH test, and conductivity test. The results drawn from these tests confirmed the identity of the Unknown White Compound to be sodium acetate (NaC2H3O2) because there were no presence of ions and sodium has a strong persistent orange color. The compound then will be synthesized with the compounds Na2CO3 and HC2H3O2 to find percent yield.
The objective of the sludge lab was to determine how many different pure substances were in the sludge by using the methods and techniques we have learned throughout the year. We had to pick separation methods so we could separate our sludge and then test characteristic properties on our separated liquids and solids. This experiment made us use our knowledge on characteristic properties to pick the ones we should test to help us identify our pure substances. Characteristic properties are properties that help identify a solid or liquid. Each solid or liquid has a certain density, boiling point, solubility, flammability, so if you know what each one is then you can use that information to help you identify your solid or liquid.
Another variable of the experiment that was controlled was the time in which the agar cubes spent in the sulphuric acid. The time allowed calculation of the rate of diffusion. The size of the agar cubes was controlled by using a grid and scalpel to, as accurately as possible, cut the agar cubes into the appropriate sizes. The shape of the agar cubes was also controlled. In future, this could be experimented with to investigate how different shaped agar blocks affect surface area to volume ratio and hence the rate of
. SUPER HYDROPHOBICITY Soumya Ranjan Sahoo (711CH1025) NIT, Rourkela Abstract: Superhydrophobicity as a sensation has turned into an increasing focus of research and technological movement, where its key viewpoints span surface chemistry, chemical physics, and cellular biology. Hydrophobic particles have a tendency to be non-polar and, accordingly, incline toward other neutral molecule and non-polar solvents. Hydrophobic atoms in water frequently bunch together, shaping micelles.
In this lab we used two processes called Diffusion and Osmosis. Diffusion is the movement of molecules from areas of high concentration to areas of low concentration. Diffusion is a process that requires no energy and involves smaller non-polar molecules. In Figure 1 you can see the molecules spreading throughout the glass from the area of high concentration, so that the areas with low concentration are filled evenly as well. The other process was osmosis.
A small amount of sand was added after the layer of cotton. After that, a layer of silica filled almost 1/3 of the column. Finally, another small amount of sand was added just above the silica. The column was given a little tap with an aspirator to make the silica more compact. Figure 2.
Lab Report Title: – Osmosis Visking tube lab Research Question: Does increasing the level of sucrose increase the procedure of osmosis? Introduction: This experiment is called the osmosis visking tube.