Five spots were labeled on the line and each amino acid standard was spotted on the plate using a capillary tube. The standards included leucine, alanine, phenylalanine, and lysine. The final spot was an unknown mixture of three amino acids. After allowing the spots to dry, the plate was placed in the developing jar and allowed to develop. The TLC setup is shown in Figure 2.
On a filter paper or cotton bud, pick the desired colonies from the agar plate. Then, using a dropper, take the oxidase reagent and drop it on to the filter paper and observe for colour changes. The blue colour appearance indicates positive reaction whereas if there are no changes, then it’s a negative reaction. 3.12.3 Genus Verification using TCBS agar The TCBS medium, known as Thiosulfate Citrate Bile Salts Sucrose Agar is a recommended selective medium which allows the growth of bacteria belonging to the genera Vibrio.
Leah Romero 10/30/2017 Conclusion Lab 3 Chem 102L In lab 3, fundamentals of chromatography, the purpose was to examine how components of mixtures can be separated by taking advantage of different in physical properties. A huge process in this lab was paper chromatography, which was used to isolate food dyes that are found in different drink mixes. The different chromatograms of FD&C dyes were compared to identify which dyes are present in each of the mixes.
Methylene chloride was added to the TLC chamber until it reaches 0.5 cm depth in order to cover the bottom of the jar; a piece of filter paper was added to the jar allowing the solvent to travel up the paper and the surface area of the solvent increased. Then the plate was placed in the jar containing 100% CH2Cl2 so that the top of the plate rested against the side of the jar opposite the filter paper. When the eluent was near to the top of TLC plate, the plate was removed and then
A small amount of n-hexane will add into the column. Silica gel weighted is mixed with n-hexane to form a mobile slurry solution and then slowly transferred into column. The side of the column will gently tape to prevent the formation of air bubbles in the column. Additional solvent will use to add the remaining silica gel into the column. Once all silica gel will add into column will gently tapped again to make the silica gel layer is flat.
7. Pour and mix the following amount of sucrose and water into 12 beakers using two different measuring cylinders, one for water and one for the sucrose solution. Look at table 1.1 as a reference to the amounts that need to be added. 8. Label each beaker with the concentration of sucrose.
You must first test the pH level of the amylase and starch solution using pH test strips, so that the experiment may be fair m. Then measure 3cm3 of amylase solution using the measuring cylinder, the pour it into the test tubes labeled A1-A5 n. Do the same for the starch solution but pour into the test tubes labeled S1-S5 o. Put test tubes A1 and S1 into the beaker labeled “cold water” p. Put test tubes A2 and S2 into the beaker labeled “normal water” q. Put test tubes A3 and S3 into the beaker labeled “warm water” r. Put test tubes A4 and S4 into the beaker labeled “very warm water” s. Put test tubes A5 and S5 into the beaker labeled “hot water” t. Mix the amylase solution with the starch solution when both are at the same temperature in each beakers (pour the amylase solution into the starch solution) u. Quickly add 3 drops of iodine solution into all 5 mixed amylase and starch solutions, while starting the stopwatch for each (should be 5 separate
Chromatography of Spinach Formal Discussion This lab involved the extraction of pigments from spinach leaves which were then analyzed using thin layer chromatography. The first step of this process was to grind up the leaves in order to extract the pigments. Hexanes facilitated this process and afterwards, the solution was dried over sodium sulfate to remove water.
After the completion of the micropipettes preparations, two silica TLC plates were prepared by drawing a line 1 cm from the edge of the silica TLC paper and divided into five parts with a ruler and a graphite pencil. One of the prepped silica TLC was used as the Reference Plate and the second as the Unknowns plate. The reference plate was spotted with Acetaminophen, Aspirin, Caffeine, Safinamide, and the standard reference mixture. The Unknowns plate was spotted with solutions identified as solution 1 through 4 and the last spot was spotted with the standard reference mixture. The micropipettes were used for spotting the two silica TLC plates after each was dipped in a separate solution.
After the four sections were placed, the lid was placed on the petri dish, and then the dish was with tape to keep close, and wrapped in aluminum foil and placed into the correct bin to sit. After two weeks the petri dishes were ready to be observed. We used a toothpick to scrap lightly the top of the agar in the petri dishes, ensuring we were collecting from one of the dividing lines. With the spores on our toothpicks, we then smeared them onto a slide that was provided to us. We had distilled water that was lightly put on top of the smear to ensure that the cover slip would stick.
Materials and Methods To start with, the unknown bacteria # 710 broth had to be successfully isolated on an EMB and MAC agar plate. Using aseptic technique by sterilizing the wire loop with Bunsen Burner between inoculations and flaming the opening of the test tubes before inserting in the loop with the bacteria. The streaking technique used was to isolate the colonies on the agar plates. In addition, the streak plates had to be incubated in a upturned position for 24 hours in a hot temperature incubator at 37 degree Celsius. Bacteria need a favorable condition to grow in.
After that, put an aluminum wire into the beaker, and after a certain period of time the solution gains color. To finish the reaction, 5 drops of 6M of Hydrochloric acid is added into the beaker to clean the solution, which means that acid dissolves all salts of aluminum that is on the solution. After finishing the chemical process, collect and use the Butcher funnel to wash the cooper because it is going to be used to a vacuum filtration. After finishing the filtration, measure the weight of the sample and dry it. Finally, Do again these two steps until notice that the subtractions of these masses are about 0.005 g, and then arrange all the chemical
The purpose of the “Titration of the Unknown Acid” lab is to determine how much of a given material known as concentration is in a substance or mixture. In this lab, the student also learns the technique of using titration. The concentration of the acid we used in class will be sampled with a standardize solution such as sodium hydroxide with an environmentally indicator to show the physical change of color that occurs to the solution by the acid. The equipment necessary for the titration experiment follows: 0.1M NaOH, Acid solution, Anthocyanin (which is found in red cabbage leaves) indicator, Burets, Ethanol 95% and DI water. First Professor Greenberg assign a labeled unknown acid solution, then we recorded the solution’s identity and bottle code.
The purpose of this laboratory experiment was to identify the molarities of dye present in green Powerade and then create a solution that possessed the same concentrations. This experiment consisted of two parts of experimentation, the first part focused on identifying the dyes present and at what concentration, and the second part focused on the recreation of the stock solution. To successfully complete this experiment, a small cuvette, full of 2 mL of green Powerade, was placed into a UV spectrometer in order to identify which wavelengths were being absorbed and reflected. With this information a complete series of dilutions using yellow #5 and blue #1 dye in ratios of 1:1, 1:2, 1:3, 1:4, and 1:5 were conducted to find the max peak absorbancy