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Control Colony Sample Lab Report

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Our results from the PCR process were very unexpected, even to the point the control colony had some rather odd outcomes. The goal of this experiment was to choose three colonies from the petri dish that has been exposed to +Amp, and look for any signs of the +Amp resistant gene, blaTEM, within the colonies and decide if this gene does have an impact on bacterial resistance towards the antibiotic. My partner and I decided to utilize a bacterial colony sample that does have blaTEM genes as our control group for us to indicate what a blaTEM gel strand would appear in the agarose gel results. When observing the product of the gel product after gel electrophoresis, we were surprised to find out none of our three colonies had any strands that indicated the presence of blaTEM despite each of them surviving through the exposure to this antibiotic. …show more content…

Colonies B and C both grew larger in comparison after the +Amp exposure, which made me come to the conclusion that it must have relatively high amount of a type of +Amp resistant gene, such as blaTEM gene. Yet, the only strand that appears close to the 828 bp strand marker (displayed by the DNA ladder) is Colony D, the control group with the known blaTEM gene. Because of this outcome, I believe that while there is no presence of the blaTEM gene, there is another type of gene that is also resistant to +Amp because the phenotype of the bacterial colonies indicated no signs of total breakdown; colonies B and C indicated signs of growth instead. In the future, I would utilize other primers during PCR analysis to test for other +Amp resistant

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