Convolvulus Pluriculis

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The second objective of our study was to observed Phenolic activity and Flavanoids estimation from Convolvulus pluricaulis. Dry leaves of Convolvulus pluricaulis were used as sample. The chemical required for the estimation were methanol, petroleum ether, di-ethyl ether, ethyl acetate, NaNO2, alcl3, NaOH, and H2SO4.The glassware’s used for the estimation were test tubes, reagent bottles, volumetric flask, Eppendrofs, falcon tubes, micropipettes, tips, test tube stand, eppendrofs stand, foil, tissue roll, autoclaved paper, blotting paper, separating funnel and spectrophotometer, cooling centrifuged, vortex, weighing balance instruments used in the present study.

Extraction via Soxhlet of Convolvulus pluricaulis dry leaves: Weighed loading limit amount of 5g of powder of drug was packed in thimble flask and 150ml of methanol (80%) was added in 250 ml round bottom flask. Then the Soxhlet assembly was set up to complete cycles until the solvent in thimble is colorless. After that the extract was filtered and filtrate was concentrated up to 50 ml using water bath. From the concentrated 10ml of extract was taken in evaporating dish (Borosilicate glass) which is previously weighed. The total …show more content…

The reaction mixture consists of 1 ml of extract and 4 ml of distilled water was taken in a 10 ml volumetric flask. To the flask, 0.30 ml of 5 % sodium nitrite was treated and after 5 minutes, 0.3 ml of 10 % aluminium chloride was mixed. After 5 minutes, 2 ml of 1M Sodium hydroxide was treated and diluted to 10 ml with distilled water. A set of reference standard solutions of quercetin (20, 40, 60, 80 and 100 μg/ml) were prepared in the same manner as described earlier. The absorbance for test and standard solutions were determined against the reagent blank at 510 nm with an UV/Visible spectrophotometer. The total flavonoid content was expressed as mg of QE/g of

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