Convolvulus Pluricaulis Lab Report

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The second objective of our study was to observed Phenolic activity and Flavanoids estimation from Convolvulus pluricaulis. Dry leaves of Convolvulus pluricaulis were used as sample. The chemical required for the estimation were methanol, petroleum ether, di-ethyl ether, ethyl acetate, NaNO2, alcl3, NaOH, and H2SO4.The glassware’s used for the estimation were test tubes, reagent bottles, volumetric flask, Eppendrofs, falcon tubes, micropipettes, tips, test tube stand, eppendrofs stand, foil, tissue roll, autoclaved paper, blotting paper, separating funnel and spectrophotometer, cooling centrifuged, vortex, weighing balance instruments used in the present study.

Extraction via Soxhlet of Convolvulus pluricaulis dry leaves: Weighed loading limit amount of 5g of powder of drug was packed in thimble flask and
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The extract filtered and filtrate evaporated or concentrated by heating at 55º C on water bath to get a paste. Then it was taken up in distilled water till it dissolved. Each of the extracts was successively extracted with equal amount of petroleum ether (40°-60°C) (fraction-I), ethyl ether (fraction-II) and ethyl acetate (fraction-III).
Each of the steps was repeated three times to ensure complete extraction in each case. Fraction I was discarded due to the presence of high fatty substances, whereas fraction II was analysed for the free flavonoids in each of the samples.
Fraction III of each of the test samples was dried and hydrolysed by refluxing with 7% H2SO4 (10 ml/gm residue) for 5 hours on water bath. The mixture was filtered and the filtrate extracted with ethyl acetate in a separating funnel. The ethyl acetate layer was washed with distilled water till neutrality and dried in vacuum. The residues were taken up in small volumes of ethanol separately and then subjected to various tests for

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