Thus, there may be low amount of amylase in saliva which cannot break down the starch completely which give dark-purple colour . In test tube C , the iodine solution change from brown to dark blue which is different from the expected . It is because the amylase is denatured at 75℃ that the the activity of amylase is low or even stop. Therefore, the starch is not broken down into maltose by amylase. In the test D, a dark-brown solution is seen in the test tube after adding the iodine as the pH of the 1ml 0.5M HCl is not an optimum pH for the activity of amylase that the starch is broken down into maltose .
Then an estimated (by trial and error) 1-3 grams of hydrated copper sulfate was added to a crucible with the lid on top. The total mass of the hydrated copper sulfate was recorded by subtracting the total mass of the crucible, lid, and sample from the mass of the crucible and lid (described in table 1.3). By attaching the wire triangle to the ring, the crucible was able to sit securely while having the bunsen burner underneath. Lighting the burner once again, each substance was heated for several minutes until estimated that the compound had changed color. Once a prevalent color change had been observed at approximately 4 minutes (blue green color) the crucible was set on the counter using the tongs to cool for approximately 5 minutes.
Gloves must be woven while preparing this solution Plasmid DNA preparation. To 20 μl of plasmid DNA preparation add 10 μl of gel loading solution and mix properly Standrad DNA marker: Take 20 μl of the Hind III DNA digest, add 10 μl of gel loading solution and mix them well. Method 1. Take a clean dry gel casting plate and make a gel mould using an adhesive tape along the sides of the plate to prevent running off of the material to be poured on the plate 2. Pour 50 ml of 1% agarose solution kept at 50ºC onto the casting plate.
V. Results and Discussion One of the objectives of this exercise is to synthesize acetylsalicylic acid (aspirin) from salicylic acid. The mechanism for this synthesis is through nucleophilic acyl substitution. Acetic anhydride was the acetylation reagent used with the salicylic acid. The mechanisms and the reaction involved in the synthesis are seen in the following figure. 1.00 gram of fine white salicylic acid powder was weighed in a clean, dry 125mL Erlenmeyer flask.
To start an experiment of adsorption isotherm, Cu(II) aqueous solution of 100 ml with the predetermined varying initial concentration of Cu(II) in the range of 6.5-370.5 mg/l and the best activator composition of NaOH was put into the erlenmeyer flask and stirred using a magnetic stirrer at 75 rpm, room temperature of 298.15 K (± 2 K), 1 atm and normal pH. The experiment was stopped at 119 mins contact time for sampling. The samples of 1 ml were placed in a 20-ml vial and diluted with 10 ml distilled water, and filtered using a syringe filter. The filtrate was placed in 10-ml vial for the AAS analysis. To determine the concentration Cu(II) in the samples from the AAS reading, dilution factor was taken into
De-ionized water was used to prepare the solutions. Photoreactor A 100mL beaker was used as the reactor having inside diameter 4.3cm and height 5.5cm. The reactor was placed on a magnetically stirred plate and a UV lamp emitting radiation of 254nm was positioned over the reactor with 15cm distance from the surface of solution. The total system was enclosed in a wooden box. This box is called the lamp house, whose inside surface was covered by aluminum foil to increase the intensity of
The resulting molten material is called the blister and contains about 99% copper by mass. (Cavette, 2007) Refining The copper blister is 99% copper but it still have a higher level of sulfure, oxygen some other impurities and by the reason it has to be further refined in order to purifies the cupper, this done by fist firing refined before it is sent to the final electro fining process • The blister copper is heated in in the refining furnace that is similar to the converter ,air is blown into the molten blister to oxides the impurities and a sodium carbonate is added to remove traces of arsenic and the antimony, • The blister copper is heated in a refining furnace, which is similar to a converter described above. Air is blown into the molten blister to oxidize some impurities. A sodium carbonate flux may be added to remove traces • Then the purified copper in then poured into the molds to form large electric
Methylene chloride was added to the TLC chamber until it reaches 0.5 cm depth in order to cover the bottom of the jar; a piece of filter paper was added to the jar allowing the solvent to travel up the paper and the surface area of the solvent increased. Then the plate was placed in the jar containing 100% CH2Cl2 so that the top of the plate rested against the side of the jar opposite the filter paper. When the eluent was near to the top of TLC plate, the plate was removed and then
Celery started with a pH of 6.05 and dropped down to a pH of 5.03 after 30 drops that is not nearly as drastic as alka seltzer. But, it shows how celery does not have a buffer because of the drop in pH and is not able to create more hydroxide ions when acid is added. Liver started with a pH of 6.50 and after 30 drops the pH dropped down to 6.03 which means the drop in pH is only .47 and looks similar to the data of the positive control of alka seltzer. The data in this lab follows the hypothesis of testing the HCI of liver and celery, then liver will contain a buffer and celery will not. This conclusion can be drawn because of celery’s large drop in pH and the data’s resemblance to the water data meaning celery cannot hydrolyze ions and keep a constant pH.