Discussion Bacteria from the provided master stock plate were used for the gram stain test. Bacteria were then streak and grown from the master stock plate onto a working plate. After an incubation time of 24 hours at 37°C, the working plate bacteria were then used to perform the catalase and red blood cell hemolysis tests. After conducting the three tests, it was concluded that unknown #5 was Streptococcus agalactiae. Gram Stain Test The Gram Stain test was first performed to differentiate the bacteria based on the thickness of the peptidoglycan layer in its cell wall.
To determine the rate of reaction there are many method to be used for example, measuring the mass after the product has been added and measuring the difference in mass on the duration of a digital scale. Another method, which will be used in this experiment is using a gas syringe to measure the volume of the gas which has been produced. The cylinder inside, will be pushed out to show a quantitative presentation of the volume produced by the reaction. Hypothesis I predict that the more baking powder added to the vinegar, the more reaction will take place causing for more gas to be produced per 10 seconds. There will be a gradual decrease in difference between volume in gas per 10 seconds over the duration of time.
A thin-layer plate is prepared by coating a glass plate with a thin film of a granular material, usually silica gel or aluminium oxide. This granular material serves as the solid stationary phase and is usually held in place on the plate with a binding agent such as plaster of Paris. If the sample to be analysed is a solid, it must first be dissolved in a suitable solvent and a few microliters of the solution spotted with a capillary tube onto the granular surface near the lower edge of the plate. A liquid sample may be applied directly to the plate in the same manner. The plate is then placed upright into a closed chamber that contains a selected liquid, with care that the liquid does not touch the sample
A safety audit for the gas-solid fluidization experiment was conducted. The following safety concerns were found when performing the audit: operating beyond the operational limits, spillage of the beads, risk of head injury from bending over to change the beads in the apparatus or from the low bar, and congestion of the workspace. One of the biggest safety concerns with this lab involved the small glass beads that acted as the solid in the experiment. If the gas flow rate goes below 50 standard cubic feet per hour (SCFH), the small glass beads could enter the flow meters. This would result in damages to the flow meters, giving false readings which in return could result in the team, conducting the experiment, to adjust flow rates beyond the operational limits that the apparatus could handle.
This increased concentration and cross linking is responsible for increased durability and make it somewhat robust. Support materials must be able to withstand the back pressures encountered during normal separations without compressing. While most commercial packing materials meet this requirement, the build-up of particulate contaminants may restrict column flow and lead to high backpressures. Under these pressures, soft porous gel supports such as agarose beads will compress and increase the pressure even further causing collapse of the support structure. More mechanically stable supports (e.g.
The purpose of this investigation was to demonstrate a relationship between change in volume and pressure. It kept the room temperature, room humidity, and mix of particles constant so that only the volume influenced the pressure. 10 tests were conducted with the volume beginning at 60 mL and decreasing at equal intervals of 4 mL, ending at 20 mL and each test had 3 trials so that the average represented a more accurate result. The results of each trial were recorded in Table 1. Then, the averages for each test were calculated and recorded in Table 2.
• To analyze the temperature dependence of viscosity by using a vibro viscometer. METHOD AND MATERIAL Capillary Viscometer By using Cannon-Fenske (U tube) viscometer, the experiment was performed for pure water and cherry juice. During this part of experiment, spherical chamber is filled with liquid and then the liquid was pulled up to upper elliptical chamber by means of puar being removed from system. Time data were recorded as well as the viscometer size. Brookfield Viscometer This procedure was followed to measure the viscosity of baby food sample by observing the shear stress vs. shear rate relationship with Brookfield viscometer.
For the experiment, the Armfield UOP12 Filtration Unit (Figure 1) was used. It consists of a plate-and-frame filter to help demonstrate filtration and the application of Darcy’s law (Equation 1). Figure 2 is a representation of the basic piping and instrumentation of the filtration unit used for the experiment. Two experiments were completed and in each experiment three runs were performed. In experiment 1, the volumetric flow rate was kept constant, whilst in experiment 2, the pressure was kept constant.
Pressure is measure within the water column of the geyser by detecting the seismic phenomenon on the surface of the geyser. The periodic change in pressure demonstrates that impulsive events in the geyser results in tremor at the geyser. And the tremor intensity is determined by the appearing frequency of these impulsive events. To further model the pressure data, Kedar and Kanamori explain the impulsive events as the collapse of spherical bubbles in the geyser. By introducing a quasi-static geyser model, they explain the bubble collapses as cooling events when water column reaches certain