Conductivity increased from 22mS cm-1 to 36mS cm-1 due to the dissolution of salts into the leachate. The conductivity reached its lowest level (9.5 mS cm-1) at day 700 and seemed to increase towards the end of the experiment (day 700). The dissolved oxygen was depleted during the first two weeks of operation reaching values that fluctuated between 0 and 0.1 mgO2 L-1 3.1.2 Biological parameters Production of biogas started slowly during the first 180 days of operation, where approximately 3 m3 of biogas were produced, mainly composed of CO2. However from day 180, the biogas production started to increase dramatically until day 500, in which period the biogas composition changed to 65%CH4 -
The aim of the investigation was to determine the effect of pH on the rate at which catalase decomposes hydrogen peroxide and consequently answer the researchable question “How does pH influence cells and consequently an organism”. The hypothesis, “As the pH deviates from 7 the initial rate of oxygen production will decrease” is supported by the results. The trend displayed in Figure 3 is, as the pH deviates from 7 the initial rate of reaction decreases. Figure 3 shows that the rate of reaction (%O2/s) decreases substantially as the pH increases and decreases to 8 and 6 respectively from a pH of 7. This is indicated as the graph shows that the initial reaction rate for pH 7 was 0.143 %O2/s compared to 0.047 and 0.053 for pH 6 and 8 respectively.
In table 1. the pH of 7 has the highest rate of O2 production being 4.41mL/min while the pH with the smallest rate of O2 production being 0.21mL/min is 4. In either direction from the pH7 the average rate is decreasing similar of that to table 2s trend. In table 2. there is a pattern from the temperature of 0.C to 100.C the average change is 2.69mL/min at 0.C then at 23.C it is at its peak being 5.5mL/min but it then decreases again at 4.4mL/min at 37.C. the trend is that in either direction of 23.C the average rate of O2 produced is decreasing. As per table 3. there is a pattern with the enzyme concentration going from 100% to 0%, at 100% the rate of O2 production is at its highest being 7.79mL/min while at 0% the rate of O2 production is at its lowest being 0mL/min.
The fastest pH was 6 (total:34.5), and it seems that there wasn’t a large change which resulted in a stable structure. The temperature in our experiment was not very high which didn’t result in denaturation of peroxidase. The temperature seemed to be a constant that didn’t affect the experiment. If the temperature was higher in pH 3 and low in pH 10, then it would cause pH 3 to denature even more which would make the pH 3 total about 4.0. Substrate concentration basically means the amount used for the substrate.
At this point, the change in pH with respect to volume was minimal since these values were far from the equivalence point, which occurred experimentally at 27.41 mL. This can also be seen on the graph as the plateau before the inflection point occured. To calculate the Ka of the acid, the following formula is
20 20 5 10 10 1.00 15.81 0.06 Graph Discussion: From the table and graph above it was observed that decrease in the concentration of hydrogen peroxide increased the time for the blue black colouration to appear, which gave rise to decrease in the rate of the reaction. The volume of distilled water added as shown in the table was to reduce the concentration of the hydrogen peroxide. The same trend was observed in the dilution of potassium Iodide. In the graph the volume of hydrogen peroxide was used as the concentration since concentration is proportional to volume. Conclusion It was clearly observed that the decrease in the concentration of hydrogen peroxide lead to increase in the reaction time and also decrease in the rate of the reaction.
Yeast undergo fermentation and produce ethanol and carbon dioxide. Carbon dioxide can be used to measure the rate of fermentation. In this study the rate of fermentation was measured through the production of carbon dioxide into a test collection tube over a 25 minute period. Fermentation increases steadily
The reaction rates were measured by Kpa/min and were written to 4 figures for precise results. Time was measured by stop watch. Table 4 shows a summary of all the groups which participated in the lab session. The similar feature is that there is increase in pressure rates with increase of drops
Microbiological water quality determinants (total bacterial count, total and fecal coliforms) were analyzed after seven days. Physical, chemical (inorganic and organic determinants such as pH, total dissolved solids, alkalinity, chlorides, Total Hardness (T.H), Iron, Manganese, Calcium, Magnesium and total trihalomethanes) were analyzed at zero time and after seven days according to (Nieuwenhuijsen., et al
An epidemiological survey showed pregnancy loss and infant mortality to be high in gas-exposed women. In a sample of 865 women who lived within 1 km of the plant and who were pregnant at the time of the gas leak, 43% of the pregnancies did not result in a live birth. Of the 486 live births, 14% of babies died in the first 30 days as compared to a death rate of 2.6 to 3% for previous deliveries in the 2 years preceding the accident in the same group of women. Gene toxicity and