MATERIALS AND METHODS Bacterial strains and culture conditions Two S. aureus strains were used in the present study; S. aureus 8325-4 (SigB-) and SH1000 representing a SigB+.strain. Overnight cultures were grown in Luria Broth (LB) at 37°Cwith shaking at 150 rpm. Exposure to antibiotics was carried out as detailed below. Antibiotics Ciprofloxacin were purchased from Sigma-Aldrich CO. 10 mg/ml stock solution of antibiotic were prepared freshly with 0.1N HCl and stored at -20°C. During the experiment we diluted with sterile water 1:10 and 1:100 depending on the different drug concentration.
Abstract- Biosurfactants are surfactants that are produced extracellular or as part of the cell membrane by bacteria, yeasts and fungi. Surfactants are compounds that reduce the surface tension of a liquid, the interfacial tension between two liquids, or that between a liquid and a solid. Surfactants are characteristically organic compounds containing both hydrophobic groups (their tails) and Hydrophilic groups (their heads). Therefore, a surfactant molecule contains both a water insoluble (and oil soluble component) and a water soluble component. Biosurfactants are the surfactants of microbial origin.
Short title: Functional Characterization of Biosurfactant Functional Characterization of Biomedical Potential of Biosurfactant Produced by Lactobacillus helveticus 1, 2Deepansh Sharma, 1*Baljeet Singh Saharan and 3Shailly Kapil 1Department of Microbiology, Kurukshetra University, Kurukshetra 136 119 INDIA 2Department of Biotechnology and Biosciences, Lovely Professional University, Jalandhar 3Dairy Microbiology Division, National Dairy Research Institute, Karnal132 001 INDIA *Baljeet.email@example.com Abstract The study aimed to investigating the functional properties of biosurfactant produced by Lactobacilli isolated from fermented foods. Biosurfactant waspartially purified by silica gel column chromatography and found as glycolipid. Biosurfactant
Gomaa (2013) further showed that biosurfactants can be used to treat many diseases and therapeutic agents, since biosurfactants are anti-adhesive to pathogens. An experiment on antimicrobial activity of biosurfactants by B. licheniformis strain M104 grown on whey as a carbon source was conducted. The results showed that biosurfactants produced from B. licheniformis strain M104 exhibited interesting antimicrobial activities against a variety of other tested
2.2. Growth conditions Escherichia coli strains were cultured and/or maintained on Luria-Bertani (LB) broth or agar media. B. bronchiseptica strains were cultured and maintained on Bordet-Gengou agar (BGA, Difco Laboratories, Detroit USA) medium supplemented with 15% v/v defibrinated sheep blood (Instituto Biológico, La Plata, Argentina). For planktonic and biofilm cultures, B. bronchiseptica was grown in Stainer-Scholte (SS) broth. When required, the growth medium was supplemented with the appropriate antibiotic at the following concentrations: streptomycin (Sm), 50 µg/ml; kanamycin (Kan),
A comprehensive description of the nature of the intermolecular interactions in binary liquid mixtures was studied combined experimental and computational approach including quantum chemistry tools and computational techniques. Many physicochemical properties of substances and many biochemicals, chemical and physical properties are regulated by hydrogen bond interaction. The study of intermolecular interactions in the liquid mixtures is of considerable importance in the explanation of the structural properties of the molecules as these interactions influences the structural arrangement of the molecules. Considerable interest has been shown in recent years in conforming and understanding the molecular hydrogen bonding interactions involved in
Biosorption of Chromium Using A. towneri and R. eutropha J. Aravind Asst. Prof. (SrG), Department of Biotechnology, Kumaraguru College of Technology, Coimbatore-641049 G. Ligi Elezabeth, T. Mariya ABSTRACT Chromium is one of the toxic heavy metals which are discharged into the environment from various industries which causes serious threat to human health as well as environment. Biosorption is one of the effective methods for removal of chromium which uses dead or inactive biological matter. In the present study, batch biosorption experiments were carried out using Acinetobacter towneri and Ralstonia eutropha. Various parameters affecting biosorption such as temperature, pH, adsorbent dosage, contact time and initial metal ion concentration
Optimization of cultural parameters for the production of antimicrobial compound from Lactobacillus fermentum (MTCC No. 1745) Sri Santhi Lanka1 and VSSL Prasad Talluri2 1. Department of Biotechnology, GITAM Institute of Science, GITAM University Visakhapatnam-530045,India. 2. Assistant Professor, Department of Biotechnology, College of Natural and Computational Science, University of Gondar, Gondar, Ethiopia *Corresponding author: Sri Santhi Lanka, E.mail firstname.lastname@example.org Abstract: To improve the productivity of antibacterial compounds of Lactobacillus fermentum by optimizing its nutrient and physical factors and screened for its antimicrobial activity by agar well diffusion method.
Results and discussion Plants are rich source of bioactive secondary metabolites which could possess antifungal potentials . B. abyssinica was tested against nine diabetic status opportunistic fungi. The results showed the plants extracts only inhibited the growth of 33% of the tested organisms. The essential oil, acetone and aqueous extracts were inactive against the growth of all organisms except M. canis, M. gypseum and T. rubrum. The zones of inhibition were varying from 19.3 to 33.3 mm (Table 1).
Solubilized drug concentration was quantified by UV spectroscopy and Insoluble drug was weighed to check the mass balance. [4,5,6] Selection of surfactant and co-surfactant: The criteria for the selection of surfactant are its HLB value, drug solubility and non-toxic nature. Several surfactants like Tween-20, Tween-80, Transcutol, PEG 400 and Cremophor EL were screened by above mentioned method. Cosurfactants were selected based on their ability to form stable and clear microemulsion at a minimum concentration. Based on this, several cosurfactants like Polyethylene Glycol 400 (PEG 400), Glycerol, Polyethylene Glycol 600 (PEG 600), Isopropyl alcohol and Isobutyl alcohol were screened.