SIM tube was used as well as the Triple Sugar Iron (TSI), MacConkey agar (MAC) and Citrate Slant. The SIM tube is used to identify hydrogen sulfide production, indole, which is a by-product of tryptophan which is broken down by tryptophanase and motility. The streaking technique used is a half stab. The TSI has an orange color and it used to identify carbohydrate fermentation specifically glucose, lactose, and sucrose fermentation. The TSI is used to observe the slant and butt of the tube as well as to identify if gas was present and if the organism produced hydrogen sulfide.
The agent used was called tetramethyl-p-phenylendiamine. When reduced the agent was clear but when oxidized it turned deep purple to a blackish color. This agent (Cytochrome C Oxidase) was the electron donor. This test did not distinguish whether the organism is oxidative or if it fermented. In the Oxidative fermentation tube the media was a differential media that helps determine whether specific bacteria can oxidize or ferment to metabolize glucose.
Remove excess water from the surface of the gram stained glass slide and observed under 1000X oil immersion microscope. Gram-staining have performed for Staphylococcus aureus (control); Enterococcus faecalis (control); Nostril microflora in NA, MSA, and PYCa. Gram-staining provides results of the bacteria morphology, type of gram-stain. Catalase test was also done prior to gram-staining. MicrobactTM Biochemical Identification Kit was used for identifying gram-negative aerobic and facultatively anaerobic bacteria.
What part do the three seeds experiment? A,Confounding variables B.Independent variables C.Control variables D.Dependent variables 40. Iodine directly helps which of the following glands to function properly? A.Pancreas B.Thyroid C.Adrenal D.Pituitary 41. Genes are made of which of the followings types of sequences that code for one or more proteins?
3.1. Materials and microorganisms Eugenol (99 %); Coniferyl Aldehyde (98 %); Ferulic Acid (99 %); Vanillin (99 %); Vanillic Acid (99 %) were obtained from Sigma Aldrich. Methanol used for high-performance liquid chromatography (HPLC) analysis was purchased from Merck and was HPLC grade. All the other chemicals used were of analytical grade and commercially available. The Pseudomonas fluorescens NCIM 2100 bacterial strain was obtained from National Collection of Industrial Microorganisms (NCIM), Pune and was provided by Department of Bio-Engineering, BIT Mesra.
The highly unsaturated ones are the omega-3 fatty acids, but another lipid found in the cell membrane is cholesterol, and all the good cholesterol found in all your cells form a hydrophobic bond. The term hydrophobic refers to the water solubility of the cell membrane, phobic meaning “afraid of” and “hydro” meaning water. So, cell membranes are secured by hydrophobic bonds, since lipids aggregate in solution without actually attaching to the atoms that constitute the solution. Shake a bottle of oil and water, and the oil (lipid) aggregates together into smaller and smaller droplets, but it will never form bonds with the water. Leave the bottle standing, and the oil always separates from the water and rises to the top, because it’s lighter and will never form bonds with water (hydrophobic).
butter •1 c. cheddar cheese Utensils: •oven (you'll need help from your adult assistant) •1½-quart casserole dish •mixing spoon Directions: 1.Heat oven to 450°F (232°C). 2.In ungreased casserole dish, stir potatoes and sauce mix, water, heavy cream, butter, and cheese. 3.Bake uncovered for about 20 minutes or until top is light golden brown and potatoes are tender. 4.Remove from
There is no correlation because there is no graph in this lab. The results look this way because the DNA made a reaction to the ethanol, and the strawberry is a living organism. This relates to the cell theory because all living things are made from cells, which have DNA, organelles, and the membranes that need to be broken down. The membranes were broken down by adding extraction buffer to the mashed up
This test distinguishes Enterobacteriaceae family. This test is done for testing bacteria ability to ferment glucose and gas production. Yellow color shows the positive result for an acid. For gas positive result bubbles are seen in the test tube. A negative result shows either reddish color or no color change and also no gas
Alyah al Mutairi Mr. Washington Biology class H 14.12.15 Deciding the behavior of amylase under the implement of excess pH levels Problem: To verify if amylase maintains to operate with the influence of hydrochloric acid Prelab: Independent variable: pH levels Dependent variable: The effect of pH enzyme action Control: Positive: Saliva and HCL+ saliva Negative: Water Constant: Iodine solution (indicator) Hypothesis: At least two out of the three procedures will be indicated as starch, due to the fact that amylase is the first enzyme that breaks down carbohydrates Materials: • Paper • Pen/pencil • Saliva • Water • Hydrochloric acid • Pipette • Iodine solution • Construction paper • Timer Procedure: 1. A sheet of paper was