Cyclodextrin Glycosyltransferase

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Paulo Roberto Martins Bonilha (2006) et. al... Cyclodextrin glycosyltransferase (EC
2.4.1.19) is an enzyme that produces cyclodextrins from starch via an intramolecular transglycosylation reaction. An alkalophilic Bacillus strain, isolated from cassava peels, was identified as Bacillus licheniformis. CGTase production by this strain was better when potato starch was used as carbon source, followed by cassava starch and amylopectin. Glucose and amylose, on the other hand, acted as synthesis repressors. When the cultivation was supplemented with sodium ions and had the pH adjusted between 6.0 and 9.0, the microorganism maintained the growth and enzyme production capacity. This data is interesting because it contradicts the concept that alkalophilic microorganisms do not grow in
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TPR71H. From this study we found that the optimized parameters for the production of
CGTase by Bacillus sp. TPR71H were Soluble Starch 3%, Yeast Extract 0.5%, K2HPO4 0.1%,
Inoculum Level 3.5%, Inoculum Age 24h, Incubation Period 36h, rpm 220, Incubation
Temperature 32°C and the pH of 7.5.
M. Manoj Saravana Guru (2012) et. al.. Cyclodextrin glucosyltransferase (CGTase) producing alkalophilic bacteria were isolated from the water samples collected from Marneri pond of Tirunelveli, Tamil Nadu by serial dilution and plating method. Totally 22 bacteria were isolated from the collected water samples and screened for CGTase activity by Horikoshis medium II agar plate method. Among 22 bacterial isolates, 15 isolates showed CGTase activity and better zone producing strain was selected for further studies. The potential strain was identified as Bacillus circulans by 16S rDNA gene sequencing experiment. The best enzyme activity was observed at pH 10.5, 32°C, supplemented with cassava as carbon source an d beef extract as nitrogen source. CGTase was purified to 20.21 fold with a yield of 55.14% recording

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