On a glass slide, 1 drop of distilled water was placed. Then, a loop-full of culture was transferred on the slide and it must be spread. It is then allowed to dry. Then, the smear must be heat fixed by exposing it to flame for few times until it got fixed. It is to prevent the cell from washing away during the staining and washing process.
Each of the analyte will have its own Rf value under certain circumstances. The separation of the phospholipid classes can be improved by two-dimensional chromatography. This technique requires developing the TLC plate in a direction, then dried, and developed in a solvent mixture at a 90 ° the first development (Singh and Jiang,
Later on the salt process was used, along with many others, to reproduce images which also opened doors to the instant developing photograph. The first step in the salt process is to coat a blank paper in silver chloride. Silver chloride is sensitive to light so when the picture is taken, and the water rinses off the chemicals, the image is captured by fine particles of silver that anchor to the paper. The salt process was used mainly in the 1840s and 50s to recreate pictures before they used the instant camera (Miller). In 1851 Fredrick Scoff Archer created the wet plate negative using a viscous solution of collodion, he covered a sheet of glass with light sensitive silver salts and the glass and salts created a more detailed photograph.
The purpose of this experiment was to identify the organism as gram-positive or gram-negative. The first step in the gram staining procedure was to heat fix the smear to the slide to ensure that the organism did not rinse off during the procedure. The second step was to apply crystal violet to the slide for twenty seconds. During this step both gram-negative and gram-positive organisms stained purple in color. After this step
For a few days after the treatment, you may feel like you have a sunburn in the treated areas. You can apply cool compresses or use a moisturizer to reduce the heat. You should use sunscreen if you plan to expose the treated area to direct sunlight. If your face was treated, you can use makeup the next day unless you have developed blisters. This is rare and they will heal in a week or two.
3. Remove slides, rinse by dipping a few times into plain buffer, then stand on end to dry. Some workers prefer to run a thin stream of tap water over the slide to remove all the remaining stain; we have not found this necessary. Be sure to wash out the coplin jars after each use. If not properly washed, stain builds up inside the jar and reduces the quality of
Develop a hypothesis on the ability of your filtration technique to remove contaminants. Hypothesis = For this dirty water can be treated it will then be clean and drinkable water 2. Based on the results of your experiment, would you reject or accept the hypothesis that you produced in question 1? Explain how you determined this. Accept/Reject = If this dirty water can be treated it will then be clean and drinkable water.
Testable Question To observe and compare single and ouble displacement reactions Hypothesis Single Displacement: If the reaction that occurs is single displacement then an increase in different reactants will result in creating a single compound/reactant. Double Displacement: If the reaction that occurs is double displacement then an increase in reactants will reesult in creating two or more compounds/reactants. Experimental Design Part A: A large well plate was placed on the lab station, each well was filled with either copper (II) nitrate, magnesium nitrate, or zinc nitrate. Added to each of the wells was three strips of copper wire, magnesium ribbon, and zinc metal (powder form). Using a toothpick, you submerged the metals in the liquid and ensured they did not touch.
In this experiment, extraction was used as a separation technique to separate the acid, base, and neutral compounds. In extraction, two immiscible solvents with different polarities are used to dissolve and separate different solute components, so they form two distinct liquid layers. In this experiment, ethyl acetate, an organic solvent, and an aqueous solution, were used as the two immiscible solvents. The extraction solvent must be capable of dissolving one of the mixture components, without irreversibly reacting with it. While initially it seems as though the organic acid, base, and neutral compounds would never pass into the aqueous layer, the conjugate acid and base of the organic base and acid respectively are soluble in water.
The daguerrotype was the first publicly announced process of photography. To make a daguerrotype, the daguerrotypist would have to polish a sheet of silver plated copper to a mirror finish and then treat it with fumes to make the surface light sensitive. To take a daguerrotype, the sheet would need to be exposed in a camera for as long as was guessed to be necessary (from just a few seconds to minutes depending on how well lit the subject was). For the sheet to be processed, it had to be treated with mercury vapour, then treat it with liquid chemical treatment to remove the light sensitivity, rinse, dry and seal the easily ruined result behind glass. What are the strengths of a
This procedure occurred in the presence a Bunsen burner. The inoculation needle were placed within the open flame 15-20 second in order to sterilize the needle and prevent contamination. The needle was allowed to cool 5-10 second before inoculation. Using aseptic transfer technique the needle was used to gather up some of the colonies on the plate, making sure not to touch anything else with the needle. The test tube was uncapped and the lips of the test tube was passed through the open flame three times.
Before starting the heating process, measure the weight of the crucible with its cover first and then tare the balance, and after that adding about 1 gram of the sample to the crucible with its cover, and then weigh it. Moreover, it is possible liberating harmful gases during the process of heating; therefore, being careful is important. The heating process ends when this sample changes the color to brown because water of hydration is removed to the sample. Additionally, give time to the small cool down and measure its weight. Next, transfer the sample to a 50 mL beaker and mixes with distilled water, which gets by rinsing the crucible with its cover in 8mL, so the solution is generated.
Then carefully measure 25ml of methanol and 25ml of ethyl acetate using a measuring beaker 12. Pour each solvent into its respective labelled mortar 13. Stir each sample for 10 minutes using a stirring rod 14. Leave solutions in the sun for 12 hours, this is to allow for the active ingredients bond with the solvent and form a solution and to allow some of the Methanol and Ethyl acetate to evaporate. 15.