Development Of SSR Markers In Satureja Bachtiarica
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Development of SSR Markers in Satureja bachtiarica , based on 454 Sequencing
Premise of the study: Microsatellite primers were developed for Satureja bachtiarica (Lamiaceae) an endemic medicinal plant of Iran. to reveal genetic characteristics in wild populations.
Methods and Results: Using next-generation sequencing, 454-GS-FLX, 15 microsatellite markers were identified, of which 11 were identified as polymorphic. These markers were study on 60 individuals from five populations of S.bachtiarica. The number of alleles per locus ranged from two to 9, with the observed heterozygosity ranging from 0.159 to 0.882.
Conclusions: :This study shows the effectiveness of SSR markers in used to detect polymorphism between S.bachtiarica populations which this markers can help to discover alleles, protection and development of germplasm for breeding programs in future.
Key words: Satureja bachtiarica; Lamiaceae; microsatellite; medicinal plant.
The genius Satureja L. (savory) belongs to the Lamiaceae family with 200 which include 30 species, is primarily distributed in Europe, West Asia, North Africa and Mediterranean basin extended to Irano-Turanian geographical regions ( Momtaz and Ablollahi, 2010. Sefidkon and Jamzad 2005; Rechinger 1982). As literature reveals, 16 species are endemic of Iran that, naturally occur in mountainous of western and northern parts in Iran. Satureja bachtiarica Bunge is a highly valued medicinal plant that among the endemic species (Rechinger, 1982,