Dexamethasone Essay

Materials and methods:

2.1. Chemicals and reagents Dexamethasone was a gift sample from mylon laboratories Limited,bangalor. Methanol (Qualigens,Mumbai.), Di-ethyl ether (fischer scientific,mumbai), tween 80 (Merck,Mumbai), 5% giemsa Staines (sigma aldrich), Phosphate buffer saline tablets (thermo fisher scientific) were purchased from national scientific products,Guntur,Andhra pradesh . Malboro cigarettes was purchased from local market,guntur,Andhra Pradesh.
2.2. Plant material
The dried leaves of Ocimum sanctum, rhizomes of Curcuma longa, flowering buds of Eugenia caryophyllus and fruits of Elettereria cardamom were collected locally. These were crushed in a grinder separately and the powders so obtained were passed through mesh sieve no to get the required coarse particles. Botanical identification was authenticated by Dr.A.Amala, Professor, department of botany, Acharya Nagarjuna University, Guntur, Andhra Pradesh, India.
2.3. Preparation of extract Two combinations were extracted, one combination contains O+C+E and second combination contains O+C+E+El. The coarse powdered mixtures were taken in equal ratios 1:1 and were packed together in soxhlet assembly and the extractions were carried out with methanol for about 72 hours. The extracts were subjected to distillation and were concentrated on a water bath. The obtained residues

Cigarette smoke extract was prepared in a specially made smoking apparatus within the college daily. A lighted cigarette was placed in the glass smoking chamber. The cigarette was burned by pumping air through the aerator tube externally. The cigarette smoke was withdrawn from the smoking chamber under negative pressure and passed through the saline within a plastic 50 ml eppendorf conical tube called collection chamber. The average burning time for cigarette was 2mintues. The resulting extract was named as 100%